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The map label for this gene is dnaN

Identifier: 88608357

GI number: 88608357

Start: 139761

End: 140879

Strand: Direct

Name: dnaN

Synonym: NSE_0170

Alternate gene names: 88608357

Gene position: 139761-140879 (Clockwise)

Preceding gene: 88608539

Following gene: 88608532

Centisome position: 16.27

GC content: 40.04

Gene sequence:

>1119_bases
ATGAAAGAAAACGCATACTCTTCCGTTACTGTGCAGCAAGATGCGTTGACAAGAATTCTTGCGAATCTTGTCAGTGTGTC
GACAAGGAAAAGCACTATAGCTTATTTGTCGCATGTTAGGGTGCGTTTCACTGGTACCTCAGTAGAGTTAACCACAACTG
ATAATGAGCTTTCACTTACGGATTCGTTGCTGGGAATTGAAGGTGTAATTGATGCAGTAATCTCAGTAGATGCGTTCAGA
TTGTATGAGATAGTCAAGCGTCTTCCAACTGATGTTGGAGTTGTTCTCTCTATTGAAAAGAATAATTTAGTTGTTCGTAG
CAGAAGAATAGAGTTCTCCTTACCATACTTAGATGCAAAACATTTTCCATTGATAAGTGAAGAGGAAAAAATTGGGCAAA
TTAACTTTGCGGTGGATGAAATAAGGAGTCTCTTCAATAAGGTGCGGTTTGCAACTTCAACTGAATCGACGAGGCAACAT
CTCAATGGTGTATATTTTGAGTCGGATGGTGAAAAAATAACCGTCGCTGCAACTGATGTACACAGGCTTGCAGTGGCCAC
ACTTATGAAAAAAACGGGTCTTAAGTTTTCTTGCATAATTCCAAAGAAGACCGTATTAGAGATGATCAAGCTTTCTTCGG
GCAAAGAAGTTGAACTCTTTCTTCATGGGAGGGAGTATGTCAGTAGGATAAAGATGGTGTTCGGGGGTGTTCGTTTAAAT
TCGAAGTTGATAGCCGGTACATTCCCAAATTTTGCTGCGGTCATTCCACACAGCTTCAAGTCTTCTCTAACATTTGATAG
AAAGGCTTTAGCGACCAGTATAGAAAGAGTTGGCCTTGTTTCACCCGATCAGAGTCGTGCAATTAGTATTGGCATTAAGG
GCAAGCAGCTTTATCTCGATGCTAGCAATTCTGATGCGAGTTTTGGGCGAGAATCATTGGAATTAAATGAAGACATCGGT
GAGGAGATTAGCATCTATTTTAATCATATTTACCTCTTGGATATACTCTTGGCTCTGGATAATAAAGAGGTGACAATGAA
GTTTAACAATGCAACTTCCCAAGTATTATTTCAGGGTGATGAAAGTGCTTTGTATGTTGTTATGCCAATGGCTTTCTAG

Upstream 100 bases:

>100_bases
TAACATACTAGTATAATCCCACACAACCCGTGCATGTGAATCTTTAGGTTCACACTATACAGATAAATTGGTATAATCCC
CGCTGTTTCTCTCCGCTAAA

Downstream 100 bases:

>100_bases
ATCTCTATGATTTTGCACCTCTCCTTCCTCCTGTGAAGAAGCATGTTTACAAAGCTAACCCTAAAGATTAGAATTTCTCT
GCTGGCCCGGTAGCTCAGTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKENAYSSVTVQQDALTRILANLVSVSTRKSTIAYLSHVRVRFTGTSVELTTTDNELSLTDSLLGIEGVIDAVISVDAFR
LYEIVKRLPTDVGVVLSIEKNNLVVRSRRIEFSLPYLDAKHFPLISEEEKIGQINFAVDEIRSLFNKVRFATSTESTRQH
LNGVYFESDGEKITVAATDVHRLAVATLMKKTGLKFSCIIPKKTVLEMIKLSSGKEVELFLHGREYVSRIKMVFGGVRLN
SKLIAGTFPNFAAVIPHSFKSSLTFDRKALATSIERVGLVSPDQSRAISIGIKGKQLYLDASNSDASFGRESLELNEDIG
EEISIYFNHIYLLDILLALDNKEVTMKFNNATSQVLFQGDESALYVVMPMAF

Sequences:

>Translated_372_residues
MKENAYSSVTVQQDALTRILANLVSVSTRKSTIAYLSHVRVRFTGTSVELTTTDNELSLTDSLLGIEGVIDAVISVDAFR
LYEIVKRLPTDVGVVLSIEKNNLVVRSRRIEFSLPYLDAKHFPLISEEEKIGQINFAVDEIRSLFNKVRFATSTESTRQH
LNGVYFESDGEKITVAATDVHRLAVATLMKKTGLKFSCIIPKKTVLEMIKLSSGKEVELFLHGREYVSRIKMVFGGVRLN
SKLIAGTFPNFAAVIPHSFKSSLTFDRKALATSIERVGLVSPDQSRAISIGIKGKQLYLDASNSDASFGRESLELNEDIG
EEISIYFNHIYLLDILLALDNKEVTMKFNNATSQVLFQGDESALYVVMPMAF
>Mature_372_residues
MKENAYSSVTVQQDALTRILANLVSVSTRKSTIAYLSHVRVRFTGTSVELTTTDNELSLTDSLLGIEGVIDAVISVDAFR
LYEIVKRLPTDVGVVLSIEKNNLVVRSRRIEFSLPYLDAKHFPLISEEEKIGQINFAVDEIRSLFNKVRFATSTESTRQH
LNGVYFESDGEKITVAATDVHRLAVATLMKKTGLKFSCIIPKKTVLEMIKLSSGKEVELFLHGREYVSRIKMVFGGVRLN
SKLIAGTFPNFAAVIPHSFKSSLTFDRKALATSIERVGLVSPDQSRAISIGIKGKQLYLDASNSDASFGRESLELNEDIG
EEISIYFNHIYLLDILLALDNKEVTMKFNNATSQVLFQGDESALYVVMPMAF

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=365, Percent_Identity=27.3972602739726, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41391; Mature: 41391

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENAYSSVTVQQDALTRILANLVSVSTRKSTIAYLSHVRVRFTGTSVELTTTDNELSLT
CCCCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCEEEEEECCCCEEHH
DSLLGIEGVIDAVISVDAFRLYEIVKRLPTDVGVVLSIEKNNLVVRSRRIEFSLPYLDAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEEEECCCCCCC
HFPLISEEEKIGQINFAVDEIRSLFNKVRFATSTESTRQHLNGVYFESDGEKITVAATDV
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCEEEEEECHH
HRLAVATLMKKTGLKFSCIIPKKTVLEMIKLSSGKEVELFLHGREYVSRIKMVFGGVRLN
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHCCEEEC
SKLIAGTFPNFAAVIPHSFKSSLTFDRKALATSIERVGLVSPDQSRAISIGIKGKQLYLD
CEEEEECCCCHHHHCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEE
ASNSDASFGRESLELNEDIGEEISIYFNHIYLLDILLALDNKEVTMKFNNATSQVLFQGD
CCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECC
ESALYVVMPMAF
CCEEEEEEEECC
>Mature Secondary Structure
MKENAYSSVTVQQDALTRILANLVSVSTRKSTIAYLSHVRVRFTGTSVELTTTDNELSLT
CCCCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHEEEEEEECCEEEEEECCCCEEHH
DSLLGIEGVIDAVISVDAFRLYEIVKRLPTDVGVVLSIEKNNLVVRSRRIEFSLPYLDAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEEEECCCCCCC
HFPLISEEEKIGQINFAVDEIRSLFNKVRFATSTESTRQHLNGVYFESDGEKITVAATDV
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEECCCCEEEEEECHH
HRLAVATLMKKTGLKFSCIIPKKTVLEMIKLSSGKEVELFLHGREYVSRIKMVFGGVRLN
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHCCEEEC
SKLIAGTFPNFAAVIPHSFKSSLTFDRKALATSIERVGLVSPDQSRAISIGIKGKQLYLD
CEEEEECCCCHHHHCCCCHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEEE
ASNSDASFGRESLELNEDIGEEISIYFNHIYLLDILLALDNKEVTMKFNNATSQVLFQGD
CCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECC
ESALYVVMPMAF
CCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]