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The map label for this gene is lipA

Identifier: 88608341

GI number: 88608341

Start: 265307

End: 266197

Strand: Direct

Name: lipA

Synonym: NSE_0322

Alternate gene names: 88608341

Gene position: 265307-266197 (Clockwise)

Preceding gene: 88608656

Following gene: 88608485

Centisome position: 30.89

GC content: 45.79

Gene sequence:

>891_bases
ATGGAACGAACGATAGAAAAGCCTAGGATTCGTGTTTCTGCGGATAATCGTGCTTTTCTCGAGACTTCCAGGCTTGTAAG
TGCATGTGGGTTAAACACTGTTTGCCAAGAGGCTGCCTGTCCGAACATTAGTGAGTGCTGGAGCAGTAAGCATGTCACCG
TGATGATTTTGGGTTCTGTCTGCACGAGGGCTTGTAGATTTTGTAATGTTACGACCGGCAAGCCTGAGTTACTCGACCCT
CATGAACCGGAAAAACTAGCTTCGGCAGTAGGTAAGCTTGGCTTGAGGCACGTTGTTATAACTTCTGTTGATAGGGATGA
TCTTGACGACGGGGGTGCAGAACATTTTGCAAGCTGCGTAAGGAGAATTAGAGAAACTTCGCCTGGTACGAGTATAGAAG
TTCTCACTCCGGACTTTCTTGGTAAGGTAGGTGCAAGGGACATCATAATAGCTGCGGCGCCGGATGTTTTCAATCATAAT
GTGGAGACTGTGCCACGTCTGCATCCTAAAATAAGGATTAAGGCAAGATACTTTAATAGTCTTTCCTTATTGGAGGAAGT
CAAAAGAAAGGATCCACGGATTTTTACGAAGTCTGGTCTAATGCTGGGACTTGGTGAGGAAAGAAGTGAGGTTTTGCAGG
TTATGGACGATATGCGTGTGGCAGGGATCGACTTTTTAACTATTGGTCAATATCTGCGGCCAAGCAAGAAACATATGGAG
GTGCAGCGCTATGCAACAGATGAGGAATTTCAATATTACAAGGAAGCTGCGTACGCACGGGGGTTTCTCATGGTTGCGTC
AAGTGCTTTGACACGTTCTTCTTATCATGCGGACGAAGATTTTTTGCATCTAAAAAATGCTAGAGCTGGGGCTCTGGCGA
AGTTGGTCTAA

Upstream 100 bases:

>100_bases
AAGGGTGGCTTGGATGATTTTCTTAGGAACACTAAAAATTCAAAACTGAGTAAGTCGGCACTAGTTTTGAAGAAGCGTAT
AATGAAGAAGGTGTCAGGTG

Downstream 100 bases:

>100_bases
ATTGTATGGTAAGCTAAGGTTTTGCAATATGCATAACTTCGTGTTTTAAGTGATCTTTGCAATGCATTGTTTAGAATTGT
CTTATTTGAACGTTTTTATT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MERTIEKPRIRVSADNRAFLETSRLVSACGLNTVCQEAACPNISECWSSKHVTVMILGSVCTRACRFCNVTTGKPELLDP
HEPEKLASAVGKLGLRHVVITSVDRDDLDDGGAEHFASCVRRIRETSPGTSIEVLTPDFLGKVGARDIIIAAAPDVFNHN
VETVPRLHPKIRIKARYFNSLSLLEEVKRKDPRIFTKSGLMLGLGEERSEVLQVMDDMRVAGIDFLTIGQYLRPSKKHME
VQRYATDEEFQYYKEAAYARGFLMVASSALTRSSYHADEDFLHLKNARAGALAKLV

Sequences:

>Translated_296_residues
MERTIEKPRIRVSADNRAFLETSRLVSACGLNTVCQEAACPNISECWSSKHVTVMILGSVCTRACRFCNVTTGKPELLDP
HEPEKLASAVGKLGLRHVVITSVDRDDLDDGGAEHFASCVRRIRETSPGTSIEVLTPDFLGKVGARDIIIAAAPDVFNHN
VETVPRLHPKIRIKARYFNSLSLLEEVKRKDPRIFTKSGLMLGLGEERSEVLQVMDDMRVAGIDFLTIGQYLRPSKKHME
VQRYATDEEFQYYKEAAYARGFLMVASSALTRSSYHADEDFLHLKNARAGALAKLV
>Mature_296_residues
MERTIEKPRIRVSADNRAFLETSRLVSACGLNTVCQEAACPNISECWSSKHVTVMILGSVCTRACRFCNVTTGKPELLDP
HEPEKLASAVGKLGLRHVVITSVDRDDLDDGGAEHFASCVRRIRETSPGTSIEVLTPDFLGKVGARDIIIAAAPDVFNHN
VETVPRLHPKIRIKARYFNSLSLLEEVKRKDPRIFTKSGLMLGLGEERSEVLQVMDDMRVAGIDFLTIGQYLRPSKKHME
VQRYATDEEFQYYKEAAYARGFLMVASSALTRSSYHADEDFLHLKNARAGALAKLV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=259, Percent_Identity=45.1737451737452, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI37577164, Length=219, Percent_Identity=45.2054794520548, Blast_Score=205, Evalue=3e-53,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=50.1805054151625, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI32564533, Length=261, Percent_Identity=44.4444444444444, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=265, Percent_Identity=47.9245283018868, Blast_Score=256, Evalue=3e-69,
Organism=Drosophila melanogaster, GI221513272, Length=259, Percent_Identity=45.945945945946, Blast_Score=244, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_NEOSM (Q2GE84)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506212.1
- ProteinModelPortal:   Q2GE84
- STRING:   Q2GE84
- GeneID:   3931843
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0322
- TIGR:   NSE_0322
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q2GE84
- ProtClustDB:   PRK05481
- BioCyc:   NSEN222891:NSE_0322-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32995; Mature: 32995

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTIEKPRIRVSADNRAFLETSRLVSACGLNTVCQEAACPNISECWSSKHVTVMILGSV
CCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCCEEEEEHHHH
CTRACRFCNVTTGKPELLDPHEPEKLASAVGKLGLRHVVITSVDRDDLDDGGAEHFASCV
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHH
RRIRETSPGTSIEVLTPDFLGKVGARDIIIAAAPDVFNHNVETVPRLHPKIRIKARYFNS
HHHHHCCCCCEEEEECHHHHHCCCCCEEEEEECCHHHCCCCHHCCCCCCCEEEEEHHHHH
LSLLEEVKRKDPRIFTKSGLMLGLGEERSEVLQVMDDMRVAGIDFLTIGQYLRPSKKHME
HHHHHHHHHCCCCEEECCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
VQRYATDEEFQYYKEAAYARGFLMVASSALTRSSYHADEDFLHLKNARAGALAKLV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MERTIEKPRIRVSADNRAFLETSRLVSACGLNTVCQEAACPNISECWSSKHVTVMILGSV
CCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHCCCCEEEEEHHHH
CTRACRFCNVTTGKPELLDPHEPEKLASAVGKLGLRHVVITSVDRDDLDDGGAEHFASCV
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHH
RRIRETSPGTSIEVLTPDFLGKVGARDIIIAAAPDVFNHNVETVPRLHPKIRIKARYFNS
HHHHHCCCCCEEEEECHHHHHCCCCCEEEEEECCHHHCCCCHHCCCCCCCEEEEEHHHHH
LSLLEEVKRKDPRIFTKSGLMLGLGEERSEVLQVMDDMRVAGIDFLTIGQYLRPSKKHME
HHHHHHHHHCCCCEEECCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHH
VQRYATDEEFQYYKEAAYARGFLMVASSALTRSSYHADEDFLHLKNARAGALAKLV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA