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The map label for this gene is ubiH [H]

Identifier: 88608325

GI number: 88608325

Start: 314001

End: 315134

Strand: Direct

Name: ubiH [H]

Synonym: NSE_0384

Alternate gene names: 88608325

Gene position: 314001-315134 (Clockwise)

Preceding gene: 88608591

Following gene: 88608800

Centisome position: 36.55

GC content: 41.27

Gene sequence:

>1134_bases
ATGACTAAGTTTTACGATTTTATAATTTCTGGGGGTGGTCTATCCGGAGTTCTAGCAGCCCATTTATTGAAGGATCTCGG
TTTGTCCTGTTACATCTTTGATAAAAGTGGTGAAGTCTCTGTTGACTTTAGAACTAGCGCGATTAGTTATGAGTCCGTGC
GTTTCTTTGAAAAGCTTGGACTGTGGCAAGTGATGGAGCCATTTGCTGTACCTATAAACGACATATACTCTTTCCAGGAG
AAAAGTGGGTCCTTTCTCCACTTTGAGGGTGCACAGGCCAATCTGGATTTTTCAATGAGCTATGTGGTGCCAAATCTGGT
GCTCCAGGATATTTTATACAAAGGAATTGATATTAATTACGGTTGTACATACAAGAATGTGTGTTATTCCCACGATAGGG
TGCGTGTGGACTTCGCAGATAAAGTGATTGAGGGAAGATATATGCTTGTTGCAGAAGGTAGGTACTCTAATACTGCAGGA
CTGCTGGGCTTCAAGATGATACGCTCAAGCTACAGACAGAGTTGTCTCATATGTAATTTGAGGAGCACTGGTAGAGAACA
CTCCAATGTTGCGGTAGAGATGTTTTTAGAAAGTGGTCCGTTTGCATTGTTACCATTACGTAAAAGCACTGAATTTTCCC
TTATCTGGACAGCTAAGTTTCCCTTTGCTAATACCCTTGCGACAATTGATGAGAAGGAGTTTCTTTCAGAGGTGCAAAAA
ATCTCGGGTATTGAGTTTGAGAAGATTCTTACACCGCGAGTAGTTTATCCTATCGTGTTAAACTTTTGCATGAATCCACG
TAAGGGGCCAGTTATGTTAATAGGCGATTCTGCACATGGGATACACCCAGTGGCCGGTCAAGGATTTAATTTGGCTGTAT
ATGATCTAAAAGATCTCCATGACGTGCTGAATAAGTATGGACTGGACAATTTTGATGAGTTGATTGACGCATATTTGAAG
AGAAGAAAACGAAGTGCTCTTTCTATGGTTGCTTTCACTCATTTCCTTGTTAAATCTTTTTCTTGTGCTTCGCCGTCTTT
ACGCGTTGCTAGAGGATTTGTATTGGATGTAATAGAATCGAGTAGTTGGTTGAAGCGTTTTTTTATGGAGCGTGCTGCCG
GAAAAGGTCTGTAG

Upstream 100 bases:

>100_bases
AAGTAGCTGAAGCTTATGCTAAAAAAAATGGTATTGACTATACTGTTGTCATGCCGCAACAAGCAAAGGTAAAGCCAAAG
TCCTACGCGGATAACTTTCA

Downstream 100 bases:

>100_bases
TGATCAATGGGATATGTTGTCTCTAAAGGTTGGGACGCGTTGTTCGCCATTAGCACTGATTCAGGCACGTCTCGTAGAGA
GAGCTCTTGCTCCCTATTGT

Product: UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTKFYDFIISGGGLSGVLAAHLLKDLGLSCYIFDKSGEVSVDFRTSAISYESVRFFEKLGLWQVMEPFAVPINDIYSFQE
KSGSFLHFEGAQANLDFSMSYVVPNLVLQDILYKGIDINYGCTYKNVCYSHDRVRVDFADKVIEGRYMLVAEGRYSNTAG
LLGFKMIRSSYRQSCLICNLRSTGREHSNVAVEMFLESGPFALLPLRKSTEFSLIWTAKFPFANTLATIDEKEFLSEVQK
ISGIEFEKILTPRVVYPIVLNFCMNPRKGPVMLIGDSAHGIHPVAGQGFNLAVYDLKDLHDVLNKYGLDNFDELIDAYLK
RRKRSALSMVAFTHFLVKSFSCASPSLRVARGFVLDVIESSSWLKRFFMERAAGKGL

Sequences:

>Translated_377_residues
MTKFYDFIISGGGLSGVLAAHLLKDLGLSCYIFDKSGEVSVDFRTSAISYESVRFFEKLGLWQVMEPFAVPINDIYSFQE
KSGSFLHFEGAQANLDFSMSYVVPNLVLQDILYKGIDINYGCTYKNVCYSHDRVRVDFADKVIEGRYMLVAEGRYSNTAG
LLGFKMIRSSYRQSCLICNLRSTGREHSNVAVEMFLESGPFALLPLRKSTEFSLIWTAKFPFANTLATIDEKEFLSEVQK
ISGIEFEKILTPRVVYPIVLNFCMNPRKGPVMLIGDSAHGIHPVAGQGFNLAVYDLKDLHDVLNKYGLDNFDELIDAYLK
RRKRSALSMVAFTHFLVKSFSCASPSLRVARGFVLDVIESSSWLKRFFMERAAGKGL
>Mature_376_residues
TKFYDFIISGGGLSGVLAAHLLKDLGLSCYIFDKSGEVSVDFRTSAISYESVRFFEKLGLWQVMEPFAVPINDIYSFQEK
SGSFLHFEGAQANLDFSMSYVVPNLVLQDILYKGIDINYGCTYKNVCYSHDRVRVDFADKVIEGRYMLVAEGRYSNTAGL
LGFKMIRSSYRQSCLICNLRSTGREHSNVAVEMFLESGPFALLPLRKSTEFSLIWTAKFPFANTLATIDEKEFLSEVQKI
SGIEFEKILTPRVVYPIVLNFCMNPRKGPVMLIGDSAHGIHPVAGQGFNLAVYDLKDLHDVLNKYGLDNFDELIDAYLKR
RKRSALSMVAFTHFLVKSFSCASPSLRVARGFVLDVIESSSWLKRFFMERAAGKGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=383, Percent_Identity=25.065274151436, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789274, Length=391, Percent_Identity=24.5524296675192, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786883, Length=392, Percent_Identity=25.2551020408163, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789273, Length=398, Percent_Identity=24.3718592964824, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 42485; Mature: 42353

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFYDFIISGGGLSGVLAAHLLKDLGLSCYIFDKSGEVSVDFRTSAISYESVRFFEKLG
CCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCC
LWQVMEPFAVPINDIYSFQEKSGSFLHFEGAQANLDFSMSYVVPNLVLQDILYKGIDINY
HHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCC
GCTYKNVCYSHDRVRVDFADKVIEGRYMLVAEGRYSNTAGLLGFKMIRSSYRQSCLICNL
CCCCCCHHCCCCEEEEEHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEC
RSTGREHSNVAVEMFLESGPFALLPLRKSTEFSLIWTAKFPFANTLATIDEKEFLSEVQK
HHCCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHH
ISGIEFEKILTPRVVYPIVLNFCMNPRKGPVMLIGDSAHGIHPVAGQGFNLAVYDLKDLH
HCCCCHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
DVLNKYGLDNFDELIDAYLKRRKRSALSMVAFTHFLVKSFSCASPSLRVARGFVLDVIES
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
SSWLKRFFMERAAGKGL
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKFYDFIISGGGLSGVLAAHLLKDLGLSCYIFDKSGEVSVDFRTSAISYESVRFFEKLG
CCEEEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHCC
LWQVMEPFAVPINDIYSFQEKSGSFLHFEGAQANLDFSMSYVVPNLVLQDILYKGIDINY
HHHHHHCCCCCHHHHHHHHHCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCC
GCTYKNVCYSHDRVRVDFADKVIEGRYMLVAEGRYSNTAGLLGFKMIRSSYRQSCLICNL
CCCCCCHHCCCCEEEEEHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEC
RSTGREHSNVAVEMFLESGPFALLPLRKSTEFSLIWTAKFPFANTLATIDEKEFLSEVQK
HHCCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHH
ISGIEFEKILTPRVVYPIVLNFCMNPRKGPVMLIGDSAHGIHPVAGQGFNLAVYDLKDLH
HCCCCHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHHH
DVLNKYGLDNFDELIDAYLKRRKRSALSMVAFTHFLVKSFSCASPSLRVARGFVLDVIES
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
SSWLKRFFMERAAGKGL
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]