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The map label for this gene is metK

Identifier: 88608304

GI number: 88608304

Start: 402908

End: 404056

Strand: Direct

Name: metK

Synonym: NSE_0488

Alternate gene names: 88608304

Gene position: 402908-404056 (Clockwise)

Preceding gene: 88608062

Following gene: 88608574

Centisome position: 46.9

GC content: 42.3

Gene sequence:

>1149_bases
ATGCATTTTGGGTTCAGGTATTTTAGCAGTGAAGCGGTTTTACCTGGTCATCCTGATAAGATAGCTGATCAGATCTCAGA
TTTGGTTTTGGACACTTATCTTCAAAAAGATCCTTCATCCAGAGTAGCAGCTGAGACAATGGTGACTCCCGATAGGGTTA
TTGTAGCTGGTGAGATCTATGGTCCAGAGGTTACCAATGCGGAACTGGAAGAAAAAATCCGCTTGTTTTTAAAAGGTATA
GGATATGAAAAACACTTATTAGACTGGGAATCTTTGGAGATAGTTAATCTTTTACATGAACAGTCCCCTGAAATTTCTGC
GGGTCTCCGGCGTTCAGCTAGTCTAGGCGCCGGTGATCAAGGCGTCATGTTTGGATATGCGACTGATGAAACACCTGTTG
GTATGCCAGCCCCGATGTATTATGCAAGGGAAATTATTCGTGTCGTGAGAGAAAGTCCTCTCTTTGGACCAGATGGGAAA
AGTCAAATTACAATTGAATACGATTTTTCTGGGAGACCAGTTGGAGTTACTAATGTGGTTGCGTCAGTTCAGCATCCCGC
AGGGATGTCTACGCGGGATGTCAGAAATACGTTGTTGGAAAAGATAGTTTCTGTTTTGCCTAAGGATTGGTCATTTTCAG
CGGATAGTATTATCATTAATCCAGCTGGTGCGTTTACAATAGGCGGTCCTAAAGGGGACTGTGGTCTGACTGGAAGAAAG
ATAGTTGCAGATACTTATGGAGGTGTAATTCCAAACGGTGGTGGTGCATTTTCCGGCAAAGACGCAACTAAAGTTGATCG
TTCTGCGGCTTATCTTGCGCGGTATATTGCAAAAAATATAGTTTTTTCAGGTGCGGCAAGCAAATGTGTTGTACAGTTAT
GTTACGCAATTGGGATGGATGCCCCACTAAATGTGCAAATCTCTCTACCCGGAATCACCGCCTGTGATGAGGAGCAGATT
TTTTCTTTAATTGTAGATAAAATTGATTTTTCTCCTTTAGGTATATCCACGCTTCTTCATCTGGATAGGCCGATATATCT
AAAGACATCGACAATAGGTCACTTTGGCCATTCATATAATCCTACGGATATGTCTTTCAAGTGGGAAGATCTAGACTTAG
TTCCTGCAATACAAGAGAGAGTAGGTTGA

Upstream 100 bases:

>100_bases
ACTAAATTCCCCTGACGCATCACCAGAGGATCATGATGGAAAAGCACAGGGCGGTATTGTATAATGCCTCCCTGAAATAT
AAAAGATTTTCCTATATTTT

Downstream 100 bases:

>100_bases
TCTCACTTTTCGCTGGCTGGCTTGCGTATAGTGACACAAATTAGTTCCTCCGTAATGTGTGAGAGTATGACCTCCGTCTC
ACTACCTAAGTCAGCACATA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MHFGFRYFSSEAVLPGHPDKIADQISDLVLDTYLQKDPSSRVAAETMVTPDRVIVAGEIYGPEVTNAELEEKIRLFLKGI
GYEKHLLDWESLEIVNLLHEQSPEISAGLRRSASLGAGDQGVMFGYATDETPVGMPAPMYYAREIIRVVRESPLFGPDGK
SQITIEYDFSGRPVGVTNVVASVQHPAGMSTRDVRNTLLEKIVSVLPKDWSFSADSIIINPAGAFTIGGPKGDCGLTGRK
IVADTYGGVIPNGGGAFSGKDATKVDRSAAYLARYIAKNIVFSGAASKCVVQLCYAIGMDAPLNVQISLPGITACDEEQI
FSLIVDKIDFSPLGISTLLHLDRPIYLKTSTIGHFGHSYNPTDMSFKWEDLDLVPAIQERVG

Sequences:

>Translated_382_residues
MHFGFRYFSSEAVLPGHPDKIADQISDLVLDTYLQKDPSSRVAAETMVTPDRVIVAGEIYGPEVTNAELEEKIRLFLKGI
GYEKHLLDWESLEIVNLLHEQSPEISAGLRRSASLGAGDQGVMFGYATDETPVGMPAPMYYAREIIRVVRESPLFGPDGK
SQITIEYDFSGRPVGVTNVVASVQHPAGMSTRDVRNTLLEKIVSVLPKDWSFSADSIIINPAGAFTIGGPKGDCGLTGRK
IVADTYGGVIPNGGGAFSGKDATKVDRSAAYLARYIAKNIVFSGAASKCVVQLCYAIGMDAPLNVQISLPGITACDEEQI
FSLIVDKIDFSPLGISTLLHLDRPIYLKTSTIGHFGHSYNPTDMSFKWEDLDLVPAIQERVG
>Mature_382_residues
MHFGFRYFSSEAVLPGHPDKIADQISDLVLDTYLQKDPSSRVAAETMVTPDRVIVAGEIYGPEVTNAELEEKIRLFLKGI
GYEKHLLDWESLEIVNLLHEQSPEISAGLRRSASLGAGDQGVMFGYATDETPVGMPAPMYYAREIIRVVRESPLFGPDGK
SQITIEYDFSGRPVGVTNVVASVQHPAGMSTRDVRNTLLEKIVSVLPKDWSFSADSIIINPAGAFTIGGPKGDCGLTGRK
IVADTYGGVIPNGGGAFSGKDATKVDRSAAYLARYIAKNIVFSGAASKCVVQLCYAIGMDAPLNVQISLPGITACDEEQI
FSLIVDKIDFSPLGISTLLHLDRPIYLKTSTIGHFGHSYNPTDMSFKWEDLDLVPAIQERVG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=379, Percent_Identity=41.688654353562, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI4557737, Length=381, Percent_Identity=40.4199475065617, Blast_Score=255, Evalue=4e-68,
Organism=Escherichia coli, GI1789311, Length=388, Percent_Identity=45.6185567010309, Blast_Score=318, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17538498, Length=377, Percent_Identity=44.0318302387268, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=43.7665782493369, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI25145633, Length=396, Percent_Identity=42.1717171717172, Blast_Score=277, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI17551082, Length=391, Percent_Identity=41.6879795396419, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI32565905, Length=375, Percent_Identity=41.0666666666667, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI32565911, Length=332, Percent_Identity=42.7710843373494, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI25145635, Length=351, Percent_Identity=40.7407407407407, Blast_Score=227, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI32565903, Length=296, Percent_Identity=44.2567567567568, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI32565909, Length=76, Percent_Identity=43.421052631579, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=379, Percent_Identity=43.0079155672823, Blast_Score=270, Evalue=4e-73,
Organism=Saccharomyces cerevisiae, GI6323209, Length=379, Percent_Identity=41.9525065963061, Blast_Score=262, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24580527, Length=378, Percent_Identity=42.8571428571429, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24580525, Length=378, Percent_Identity=42.8571428571429, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI45552159, Length=378, Percent_Identity=43.1216931216931, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24580529, Length=378, Percent_Identity=43.1216931216931, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24580537, Length=378, Percent_Identity=43.1216931216931, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24580533, Length=378, Percent_Identity=43.1216931216931, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24580535, Length=378, Percent_Identity=43.1216931216931, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24580531, Length=378, Percent_Identity=43.1216931216931, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24580541, Length=376, Percent_Identity=41.7553191489362, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=38.135593220339, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41418; Mature: 41418

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFGFRYFSSEAVLPGHPDKIADQISDLVLDTYLQKDPSSRVAAETMVTPDRVIVAGEIY
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEEC
GPEVTNAELEEKIRLFLKGIGYEKHLLDWESLEIVNLLHEQSPEISAGLRRSASLGAGDQ
CCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
GVMFGYATDETPVGMPAPMYYAREIIRVVRESPLFGPDGKSQITIEYDFSGRPVGVTNVV
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHH
ASVQHPAGMSTRDVRNTLLEKIVSVLPKDWSFSADSIIINPAGAFTIGGPKGDCGLTGRK
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCE
IVADTYGGVIPNGGGAFSGKDATKVDRSAAYLARYIAKNIVFSGAASKCVVQLCYAIGMD
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
APLNVQISLPGITACDEEQIFSLIVDKIDFSPLGISTLLHLDRPIYLKTSTIGHFGHSYN
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCHHCCCCCCC
PTDMSFKWEDLDLVPAIQERVG
CCCCEEEECCCCCCHHHHHHCC
>Mature Secondary Structure
MHFGFRYFSSEAVLPGHPDKIADQISDLVLDTYLQKDPSSRVAAETMVTPDRVIVAGEIY
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEEC
GPEVTNAELEEKIRLFLKGIGYEKHLLDWESLEIVNLLHEQSPEISAGLRRSASLGAGDQ
CCCCCCHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
GVMFGYATDETPVGMPAPMYYAREIIRVVRESPLFGPDGKSQITIEYDFSGRPVGVTNVV
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHH
ASVQHPAGMSTRDVRNTLLEKIVSVLPKDWSFSADSIIINPAGAFTIGGPKGDCGLTGRK
HHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCE
IVADTYGGVIPNGGGAFSGKDATKVDRSAAYLARYIAKNIVFSGAASKCVVQLCYAIGMD
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
APLNVQISLPGITACDEEQIFSLIVDKIDFSPLGISTLLHLDRPIYLKTSTIGHFGHSYN
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCHHCCCCCCC
PTDMSFKWEDLDLVPAIQERVG
CCCCEEEECCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA