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The map label for this gene is pyrD

Identifier: 88608290

GI number: 88608290

Start: 148110

End: 149156

Strand: Direct

Name: pyrD

Synonym: NSE_0179

Alternate gene names: 88608290

Gene position: 148110-149156 (Clockwise)

Preceding gene: 88608617

Following gene: 88608490

Centisome position: 17.24

GC content: 42.31

Gene sequence:

>1047_bases
ATGCATGAGAAGCTTTTAGTGGCACTTCTGCAGCGTTTGAACCCGTCGTTTGCGCATGATGTGACTCGCGTGGTACTCAA
GCATTTTCCGGGATTTGTAAGCCAAAATTCTTTTGAGGACCCGATTCTGGAAAGTAGGGTGTGTGGTTTGCATTTTAAAA
ACCCAATTGGTATTGCAGCTGGATTTGATAAGAATGCTGAATGTGTTACTGCGCTCAGGAGAGTAGGATTTGGCTTCATT
GAGCTTGGAACCGTCACACTTAAACCGCAAAAGGGGAACCCCAGTCCTCGATTGTTTAGATTGCCGCAGGACGGTGCCAT
CATTAATCGACTTGGATTTAATAATAAAGGGATAGCCTATTTCCTCAAATCGCTTAAGAAAGCTATGACACACATGGATT
TGCCACCTTTAGGAGTTAATATCGGAAAAAATGCTGTTTCAAGTAATGCCGTTTCTGATTATGCACGCTTGGCTGCCTAT
GTCTCCTCGGTTGCCGACTATATCACGCTAAATATCTCTTCTCCGAATACTGTTAGTCTCAGGGATATGCAAAAAGTGGA
GATCCTTGAGGAACTTCTTATTGCTGTAAAGGCAGCAGTTGGAAGTAATGTACGGATTTTTATCAAGGTGGCTCCTGATC
TTGCTGAGGGAGCAGAGGTCGGTATTATTTCGCTTGCTTTGAAGCACAAAGTAGCCGGGATCATAGTAAGTAATACTACT
ATTGGATGTAGGGAAAGCCTTCATAGTAAGCATAAAGTCGAATACGGTGGTTTAAGTGGTACACCACTGTTTAAACTTTC
TACAGGTTTGTTGAGAAAAATGTATAGGTGCACAAACGGGGAATTGGTTTTCATAGGTTGTGGTGGCATTTCAGATGCAG
AAACGGCTTATGCGAAAATTAGAAATGGTGCTGCTTTAATTCAAGCTTATACGAGTTTCACCTATCATGGTTTTGGCCTT
CTGAACCAGATCAAAAAAGATTTGGTTCAGAGACTTAAGGCTGATGGTTTTTCTTCTGTAAGTGAGGCTGTAGGTGTGGA
TGCGTAA

Upstream 100 bases:

>100_bases
GCTTGTACTTTTCGCTTCTTGCGTTTTATTTATCTATTCCATCTTGAAAATTTTCAGAAAGTGACGGAAAATTCGCAGGT
CTCTCGCTTTTCTGAGTAAA

Downstream 100 bases:

>100_bases
TTTTAGATTCCGGTTTTAATCCTCACATATAAATTTTGCAACGGGTAGGGAGTTTTTATGTGTAAAACTTAGTATGTACT
TCCAATACAGCTATTGTTTT

Product: dihydroorotate dehydrogenase

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MHEKLLVALLQRLNPSFAHDVTRVVLKHFPGFVSQNSFEDPILESRVCGLHFKNPIGIAAGFDKNAECVTALRRVGFGFI
ELGTVTLKPQKGNPSPRLFRLPQDGAIINRLGFNNKGIAYFLKSLKKAMTHMDLPPLGVNIGKNAVSSNAVSDYARLAAY
VSSVADYITLNISSPNTVSLRDMQKVEILEELLIAVKAAVGSNVRIFIKVAPDLAEGAEVGIISLALKHKVAGIIVSNTT
IGCRESLHSKHKVEYGGLSGTPLFKLSTGLLRKMYRCTNGELVFIGCGGISDAETAYAKIRNGAALIQAYTSFTYHGFGL
LNQIKKDLVQRLKADGFSSVSEAVGVDA

Sequences:

>Translated_348_residues
MHEKLLVALLQRLNPSFAHDVTRVVLKHFPGFVSQNSFEDPILESRVCGLHFKNPIGIAAGFDKNAECVTALRRVGFGFI
ELGTVTLKPQKGNPSPRLFRLPQDGAIINRLGFNNKGIAYFLKSLKKAMTHMDLPPLGVNIGKNAVSSNAVSDYARLAAY
VSSVADYITLNISSPNTVSLRDMQKVEILEELLIAVKAAVGSNVRIFIKVAPDLAEGAEVGIISLALKHKVAGIIVSNTT
IGCRESLHSKHKVEYGGLSGTPLFKLSTGLLRKMYRCTNGELVFIGCGGISDAETAYAKIRNGAALIQAYTSFTYHGFGL
LNQIKKDLVQRLKADGFSSVSEAVGVDA
>Mature_348_residues
MHEKLLVALLQRLNPSFAHDVTRVVLKHFPGFVSQNSFEDPILESRVCGLHFKNPIGIAAGFDKNAECVTALRRVGFGFI
ELGTVTLKPQKGNPSPRLFRLPQDGAIINRLGFNNKGIAYFLKSLKKAMTHMDLPPLGVNIGKNAVSSNAVSDYARLAAY
VSSVADYITLNISSPNTVSLRDMQKVEILEELLIAVKAAVGSNVRIFIKVAPDLAEGAEVGIISLALKHKVAGIIVSNTT
IGCRESLHSKHKVEYGGLSGTPLFKLSTGLLRKMYRCTNGELVFIGCGGISDAETAYAKIRNGAALIQAYTSFTYHGFGL
LNQIKKDLVQRLKADGFSSVSEAVGVDA

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI45006951, Length=357, Percent_Identity=42.0168067226891, Blast_Score=269, Evalue=3e-72,
Organism=Escherichia coli, GI1787177, Length=338, Percent_Identity=39.6449704142012, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17509475, Length=323, Percent_Identity=45.2012383900929, Blast_Score=270, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322633, Length=325, Percent_Identity=25.5384615384615, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI281361352, Length=346, Percent_Identity=41.0404624277457, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17137316, Length=346, Percent_Identity=41.0404624277457, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24640763, Length=319, Percent_Identity=26.0188087774295, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18858217, Length=319, Percent_Identity=26.0188087774295, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295 [H]

Pfam domain/function: PF01180 DHO_dh [H]

EC number: =1.3.5.2 [H]

Molecular weight: Translated: 37572; Mature: 37572

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00911 DHODEHASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEKLLVALLQRLNPSFAHDVTRVVLKHFPGFVSQNSFEDPILESRVCGLHFKNPIGIAA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCEECCCCCEEE
GFDKNAECVTALRRVGFGFIELGTVTLKPQKGNPSPRLFRLPQDGAIINRLGFNNKGIAY
CCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEECCCCCCEEHHCCCCCCCHHH
FLKSLKKAMTHMDLPPLGVNIGKNAVSSNAVSDYARLAAYVSSVADYITLNISSPNTVSL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEH
RDMQKVEILEELLIAVKAAVGSNVRIFIKVAPDLAEGAEVGIISLALKHKVAGIIVSNTT
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEECCC
IGCRESLHSKHKVEYGGLSGTPLFKLSTGLLRKMYRCTNGELVFIGCGGISDAETAYAKI
HHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
RNGAALIQAYTSFTYHGFGLLNQIKKDLVQRLKADGFSSVSEAVGVDA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MHEKLLVALLQRLNPSFAHDVTRVVLKHFPGFVSQNSFEDPILESRVCGLHFKNPIGIAA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCEECCCCCEEE
GFDKNAECVTALRRVGFGFIELGTVTLKPQKGNPSPRLFRLPQDGAIINRLGFNNKGIAY
CCCCCHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCEEECCCCCCEEHHCCCCCCCHHH
FLKSLKKAMTHMDLPPLGVNIGKNAVSSNAVSDYARLAAYVSSVADYITLNISSPNTVSL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEH
RDMQKVEILEELLIAVKAAVGSNVRIFIKVAPDLAEGAEVGIISLALKHKVAGIIVSNTT
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEECCC
IGCRESLHSKHKVEYGGLSGTPLFKLSTGLLRKMYRCTNGELVFIGCGGISDAETAYAKI
HHHHHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
RNGAALIQAYTSFTYHGFGLLNQIKKDLVQRLKADGFSSVSEAVGVDA
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA