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The map label for this gene is recF [H]

Identifier: 88608256

GI number: 88608256

Start: 169119

End: 170168

Strand: Direct

Name: recF [H]

Synonym: NSE_0199

Alternate gene names: 88608256

Gene position: 169119-170168 (Clockwise)

Preceding gene: 88608156

Following gene: 88608057

Centisome position: 19.69

GC content: 40.95

Gene sequence:

>1050_bases
ATGAGGCCTTATATTACGGGGGTCTTGGTTAAGGATTTCCGTAATCATGCTTTTTGGACGGGGTCATTTAAATGCAGGCA
TGTCTTGCTTTGCGGTAAAAACGGTGCCGGCAAAACAAGTATACTCGAAGCTATATCAAAGTTATCTCCTGGGTTGGGTC
TCAGGTCTGCGAGTAATATCGAGATGGTTAGATCTGGGTCTCTTTCTTGGGAGGTAAGTCTGAAATTTGCCGGTAGCACT
GACTTAAGGGAAGTCGGAATGGGCTATTATGAGGATAAACGAGTTACTAAATTAAATGGGAAGTCCATTCAGTGTTTCAA
GAAGGTAATTGACCTTGTCAAGGTTATGTGGCTCACCCCGCAGATGTCTAATTTGTTTACCACTGATAAGTCTGTTAGGA
GAAAGTTTTTTGACCGTATGGTTGCACTTTCTGAACCTCAGCACCTTGAGAATCTTGTGATGTACGAACGTTTTAAGTCA
GAACGTCTGAAGATTCTTAACGCAGGTGCGTCCAAAATGTGGTTAGATGTTAACGAAAAAAAACTAGCAGAGTTATGCAT
AGCTATTACTGATGCCAGAGTGAGTTTCATCGGACAACTGATGAGTAATTTCCCTTCTAAAGGTTTTGGTTCTCCAGAAA
TTAAATTGTTCTGTCCTGTCGCAAGTGCAATTGGTAGGGTAGGTTCTTCTCAGCAGATGCAGTGTATTCAGTCCGCTCTT
GAAAGAAGTAGAGCTATCGATACTGTCACTGGGAAGATGCAATTTGGTGTACACCGAACAGACTTTCTTGCTACAGTACG
ACAAGGAGACAATACCGCTAGATGCTATTCTACTGGGGAGCAAAAGTTACTTATTTTAGGGATAATACTTGCTGCCGGTG
AACTAATAGACATAATACTTCTAGATGATATTTTTGCTCACCTAGATCTGCATAACTCTTCAGCCTTTCTTTTGGAGGCA
ACTAAAAAAAATTGTCAGTTTTTTTTTAGTGATCTTGACAACAGCAAGTTCGCGCAGTTTGCAAATGTGATACATACTAT
TCCGGTTTAG

Upstream 100 bases:

>100_bases
TTGATCCTTTCCAGACGGTGCTTAACATTTTTGGTAATGGTGCTTTCGCGATTTTTTTCAGTAAGCTGTACAAGGGTAAA
AGGACAGCTACAGTTAGCAA

Downstream 100 bases:

>100_bases
TAGTTAGACTGATCTCGGGACGTGTATCGTGATGCTGAATGTACAGTTTGGGTCTGATACGTCGTGCTAGTAAGTACTGA
GAAGGGTAGTAGACTGTGAT

Product: putative DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRPYITGVLVKDFRNHAFWTGSFKCRHVLLCGKNGAGKTSILEAISKLSPGLGLRSASNIEMVRSGSLSWEVSLKFAGST
DLREVGMGYYEDKRVTKLNGKSIQCFKKVIDLVKVMWLTPQMSNLFTTDKSVRRKFFDRMVALSEPQHLENLVMYERFKS
ERLKILNAGASKMWLDVNEKKLAELCIAITDARVSFIGQLMSNFPSKGFGSPEIKLFCPVASAIGRVGSSQQMQCIQSAL
ERSRAIDTVTGKMQFGVHRTDFLATVRQGDNTARCYSTGEQKLLILGIILAAGELIDIILLDDIFAHLDLHNSSAFLLEA
TKKNCQFFFSDLDNSKFAQFANVIHTIPV

Sequences:

>Translated_349_residues
MRPYITGVLVKDFRNHAFWTGSFKCRHVLLCGKNGAGKTSILEAISKLSPGLGLRSASNIEMVRSGSLSWEVSLKFAGST
DLREVGMGYYEDKRVTKLNGKSIQCFKKVIDLVKVMWLTPQMSNLFTTDKSVRRKFFDRMVALSEPQHLENLVMYERFKS
ERLKILNAGASKMWLDVNEKKLAELCIAITDARVSFIGQLMSNFPSKGFGSPEIKLFCPVASAIGRVGSSQQMQCIQSAL
ERSRAIDTVTGKMQFGVHRTDFLATVRQGDNTARCYSTGEQKLLILGIILAAGELIDIILLDDIFAHLDLHNSSAFLLEA
TKKNCQFFFSDLDNSKFAQFANVIHTIPV
>Mature_349_residues
MRPYITGVLVKDFRNHAFWTGSFKCRHVLLCGKNGAGKTSILEAISKLSPGLGLRSASNIEMVRSGSLSWEVSLKFAGST
DLREVGMGYYEDKRVTKLNGKSIQCFKKVIDLVKVMWLTPQMSNLFTTDKSVRRKFFDRMVALSEPQHLENLVMYERFKS
ERLKILNAGASKMWLDVNEKKLAELCIAITDARVSFIGQLMSNFPSKGFGSPEIKLFCPVASAIGRVGSSQQMQCIQSAL
ERSRAIDTVTGKMQFGVHRTDFLATVRQGDNTARCYSTGEQKLLILGIILAAGELIDIILLDDIFAHLDLHNSSAFLLEA
TKKNCQFFFSDLDNSKFAQFANVIHTIPV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=320, Percent_Identity=24.375, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 38985; Mature: 38985

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPYITGVLVKDFRNHAFWTGSFKCRHVLLCGKNGAGKTSILEAISKLSPGLGLRSASNI
CCCHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCC
EMVRSGSLSWEVSLKFAGSTDLREVGMGYYEDKRVTKLNGKSIQCFKKVIDLVKVMWLTP
HHEECCCCEEEEEEEECCCCCHHHHCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHCCC
QMSNLFTTDKSVRRKFFDRMVALSEPQHLENLVMYERFKSERLKILNAGASKMWLDVNEK
CHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHH
KLAELCIAITDARVSFIGQLMSNFPSKGFGSPEIKLFCPVASAIGRVGSSQQMQCIQSAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHH
ERSRAIDTVTGKMQFGVHRTDFLATVRQGDNTARCYSTGEQKLLILGIILAAGELIDIIL
HHHHHHHHHCCCCEECCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
LDDIFAHLDLHNSSAFLLEATKKNCQFFFSDLDNSKFAQFANVIHTIPV
HHHHHHHHCCCCCCEEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPYITGVLVKDFRNHAFWTGSFKCRHVLLCGKNGAGKTSILEAISKLSPGLGLRSASNI
CCCHHHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCC
EMVRSGSLSWEVSLKFAGSTDLREVGMGYYEDKRVTKLNGKSIQCFKKVIDLVKVMWLTP
HHEECCCCEEEEEEEECCCCCHHHHCCCEECCCEEEEECCCHHHHHHHHHHHHHHHHCCC
QMSNLFTTDKSVRRKFFDRMVALSEPQHLENLVMYERFKSERLKILNAGASKMWLDVNEK
CHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCHH
KLAELCIAITDARVSFIGQLMSNFPSKGFGSPEIKLFCPVASAIGRVGSSQQMQCIQSAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHH
ERSRAIDTVTGKMQFGVHRTDFLATVRQGDNTARCYSTGEQKLLILGIILAAGELIDIIL
HHHHHHHHHCCCCEECCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHH
LDDIFAHLDLHNSSAFLLEATKKNCQFFFSDLDNSKFAQFANVIHTIPV
HHHHHHHHCCCCCCEEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]