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The map label for this gene is groEL

Identifier: 88608250

GI number: 88608250

Start: 561722

End: 563368

Strand: Reverse

Name: groEL

Synonym: NSE_0642

Alternate gene names: 88608250

Gene position: 563368-561722 (Counterclockwise)

Preceding gene: 88608649

Following gene: 88608734

Centisome position: 65.58

GC content: 44.14

Gene sequence:

>1647_bases
ATGGCGAACGAAGTAATAAGTGGTGAACAGTTGCAGAGAGTTATCCGTGATGCAGCTGACTTGGTTGTATCAAGTGCTGG
TGTAACACTTGGGCCTGAGGGTAGACCAGTCATAATGAGTAAGTCTTATGGTGGCCCGGAAGTAACCAAGGATGGTTATA
AGGTGATGAATCAGCTTAAACCGGAAGATGAGAAGGTTGCAAAGATAGTCGAGCTATTAAACCAGGCTACCTCACAGGCG
AATGAAAAAGCTGGAGATGGGACTACTACTGCTACTATCTTGGTGGGCAACATGATCAAAAATGCTCACAAACACATTGC
TGCTCAAAGGTCCCGGATGAAGCTTAAATCCGGTATGAAACGTGCACGCGACGAGGTCGTGAAGTATATTCAATCAGTTG
CCAAGAAGATAAATTCGGAAGAAGAGATAGCACAAGTTGGTTCAATTTCTGCTAACGGCAATAGTGAGATTGGTGGCAAG
ATTGCCGAAGCTATGAATAAAGTTGGTAAAGAGGGTGTTATTACTGTCGAAGAAGGTAAAGGATTAGATGAGTTTTCTGT
ATCCGTTGTGCAAGGTATGGTCTTCGACAGAGGATACGTTTCTCCTTATTTTATAACAAACCCGGAGAAAATGATTGTTG
AGTTTGACAATCCTTATGTCCTTCTCGCGAATAAAAAACTCTCTAGCATACAACCAATGGTTCCTCTTTTGGAAACGATT
GTTCGTTCGAACAGAGCAGTCGTCATAATCGCTGAGGATGTTGAGGGTGAAGCTCTTACTTCTCTTGTTCTTAGCAAAAT
GAGGGGTAGCTTGAAAGCTTGCGCTGTTAAAGCTCCAGGCTTCGGTGATCGCAGATCTGAGATGCTCGAAGACATTAGGA
TACTTACCGGTGCTAAAACACTAGTAAGTGACGATTTGGGCGTGACAGTTGAGAGCCTTACTGTTGAAGATCTTGGTACT
GCAAAGAGCATCATTATATCTAAGGATTCCACGACTATAGTAGATGGTGGTGGCGAAAAAACAGCAATTGAAGCGAGGGT
CAAGCAGATTAAGACACAAATAGACAAGACTACTTCTGATTACGACAAGGAGAAACTTCAGGAGAGACTTGCTAAACTTG
CTGGTGGTGTTGCTGTCCTCAAGGTTGGTGGTGCTACAGAAGTTGAGGTTAAAGAACGCAAAGATCGTGTAGAAGATGCT
CTACATGCTACACGTGCCGCAGTTGAGGAAGGTATAGTTCCTGGTGGTGGTGCTACATTGCTTAGTGCAATCGCTGTTTT
GGAAAAGCTCTCTTCTGATGATGATGATGAGCAGGCTGGTATTAACATCGTAAAGACGGCACTCAAGGCGCCTATTTCTC
AGATTGTTGAAAATGCCGGTGAAGATGCTTCTGTGATCACATACAATCTTCTGGAATCAAAGGATCCTAACAGGATCTTC
GATGCACGCGAGCTTAAGTATGTAGATGCCTTCAAGGCAGGCATTATAGATCCAGCGAAGGTAGTGCGTGTCGCACTGGA
AAGTGCTGTTTCTGTTGCTAGTGTTCTAGTGACTACCGAAGCCCTAATAGTTGATTTGCCGACTAAAGATAACGGTTCTT
CTTCAATGATGCCTGGTGGTGGCATGGGTGGAATGGGCGGTTTCTAA

Upstream 100 bases:

>100_bases
TTCTTTACAGAAAATGGGCAGGCAGTGAAGTGGAGCATGACGGTGTTGAGTACGTCGTGATGAAGGAGACTGATATTATT
GCTATTAAGGAGGGTAAATA

Downstream 100 bases:

>100_bases
TTGCTTGTTGGGAGGGGTACTCTGGGCCCCTCCTGCTTATCCCTTGTGTTATTTGATCCAGGTAGTCTTCGTTCTGGATG
GGGTTGGTTCAGGTGTCTCA

Product: 60 kDa chaperonin

Products: NA

Alternate protein names: 55 kDa major antigen; GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MANEVISGEQLQRVIRDAADLVVSSAGVTLGPEGRPVIMSKSYGGPEVTKDGYKVMNQLKPEDEKVAKIVELLNQATSQA
NEKAGDGTTTATILVGNMIKNAHKHIAAQRSRMKLKSGMKRARDEVVKYIQSVAKKINSEEEIAQVGSISANGNSEIGGK
IAEAMNKVGKEGVITVEEGKGLDEFSVSVVQGMVFDRGYVSPYFITNPEKMIVEFDNPYVLLANKKLSSIQPMVPLLETI
VRSNRAVVIIAEDVEGEALTSLVLSKMRGSLKACAVKAPGFGDRRSEMLEDIRILTGAKTLVSDDLGVTVESLTVEDLGT
AKSIIISKDSTTIVDGGGEKTAIEARVKQIKTQIDKTTSDYDKEKLQERLAKLAGGVAVLKVGGATEVEVKERKDRVEDA
LHATRAAVEEGIVPGGGATLLSAIAVLEKLSSDDDDEQAGINIVKTALKAPISQIVENAGEDASVITYNLLESKDPNRIF
DARELKYVDAFKAGIIDPAKVVRVALESAVSVASVLVTTEALIVDLPTKDNGSSSMMPGGGMGGMGGF

Sequences:

>Translated_548_residues
MANEVISGEQLQRVIRDAADLVVSSAGVTLGPEGRPVIMSKSYGGPEVTKDGYKVMNQLKPEDEKVAKIVELLNQATSQA
NEKAGDGTTTATILVGNMIKNAHKHIAAQRSRMKLKSGMKRARDEVVKYIQSVAKKINSEEEIAQVGSISANGNSEIGGK
IAEAMNKVGKEGVITVEEGKGLDEFSVSVVQGMVFDRGYVSPYFITNPEKMIVEFDNPYVLLANKKLSSIQPMVPLLETI
VRSNRAVVIIAEDVEGEALTSLVLSKMRGSLKACAVKAPGFGDRRSEMLEDIRILTGAKTLVSDDLGVTVESLTVEDLGT
AKSIIISKDSTTIVDGGGEKTAIEARVKQIKTQIDKTTSDYDKEKLQERLAKLAGGVAVLKVGGATEVEVKERKDRVEDA
LHATRAAVEEGIVPGGGATLLSAIAVLEKLSSDDDDEQAGINIVKTALKAPISQIVENAGEDASVITYNLLESKDPNRIF
DARELKYVDAFKAGIIDPAKVVRVALESAVSVASVLVTTEALIVDLPTKDNGSSSMMPGGGMGGMGGF
>Mature_547_residues
ANEVISGEQLQRVIRDAADLVVSSAGVTLGPEGRPVIMSKSYGGPEVTKDGYKVMNQLKPEDEKVAKIVELLNQATSQAN
EKAGDGTTTATILVGNMIKNAHKHIAAQRSRMKLKSGMKRARDEVVKYIQSVAKKINSEEEIAQVGSISANGNSEIGGKI
AEAMNKVGKEGVITVEEGKGLDEFSVSVVQGMVFDRGYVSPYFITNPEKMIVEFDNPYVLLANKKLSSIQPMVPLLETIV
RSNRAVVIIAEDVEGEALTSLVLSKMRGSLKACAVKAPGFGDRRSEMLEDIRILTGAKTLVSDDLGVTVESLTVEDLGTA
KSIIISKDSTTIVDGGGEKTAIEARVKQIKTQIDKTTSDYDKEKLQERLAKLAGGVAVLKVGGATEVEVKERKDRVEDAL
HATRAAVEEGIVPGGGATLLSAIAVLEKLSSDDDDEQAGINIVKTALKAPISQIVENAGEDASVITYNLLESKDPNRIFD
ARELKYVDAFKAGIIDPAKVVRVALESAVSVASVLVTTEALIVDLPTKDNGSSSMMPGGGMGGMGGF

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=529, Percent_Identity=44.6124763705104, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI31542947, Length=529, Percent_Identity=44.6124763705104, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1790586, Length=503, Percent_Identity=51.2922465208747, Blast_Score=502, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17555558, Length=526, Percent_Identity=45.8174904942966, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI193210679, Length=221, Percent_Identity=51.131221719457, Blast_Score=214, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6323288, Length=500, Percent_Identity=49.4, Blast_Score=466, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24641193, Length=529, Percent_Identity=43.4782608695652, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24641191, Length=529, Percent_Identity=43.4782608695652, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45550936, Length=530, Percent_Identity=42.0754716981132, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45550132, Length=530, Percent_Identity=42.0754716981132, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45550935, Length=530, Percent_Identity=42.0754716981132, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI17864606, Length=525, Percent_Identity=40.7619047619048, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24584129, Length=533, Percent_Identity=33.0206378986867, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI19921262, Length=533, Percent_Identity=33.0206378986867, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 58229; Mature: 58098

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEVISGEQLQRVIRDAADLVVSSAGVTLGPEGRPVIMSKSYGGPEVTKDGYKVMNQLK
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCHHHHHHHHHCC
PEDEKVAKIVELLNQATSQANEKAGDGTTTATILVGNMIKNAHKHIAAQRSRMKLKSGMK
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RARDEVVKYIQSVAKKINSEEEIAQVGSISANGNSEIGGKIAEAMNKVGKEGVITVEEGK
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
GLDEFSVSVVQGMVFDRGYVSPYFITNPEKMIVEFDNPYVLLANKKLSSIQPMVPLLETI
CCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCEEEEECCCHHHCCHHHHHHHHH
VRSNRAVVIIAEDVEGEALTSLVLSKMRGSLKACAVKAPGFGDRRSEMLEDIRILTGAKT
HCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHCCHH
LVSDDLGVTVESLTVEDLGTAKSIIISKDSTTIVDGGGEKTAIEARVKQIKTQIDKTTSD
HHCCCCCCEEEEEEHHHCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCH
YDKEKLQERLAKLAGGVAVLKVGGATEVEVKERKDRVEDALHATRAAVEEGIVPGGGATL
HHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LSAIAVLEKLSSDDDDEQAGINIVKTALKAPISQIVENAGEDASVITYNLLESKDPNRIF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCHHC
DARELKYVDAFKAGIIDPAKVVRVALESAVSVASVLVTTEALIVDLPTKDNGSSSMMPGG
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCC
GMGGMGGF
CCCCCCCC
>Mature Secondary Structure 
ANEVISGEQLQRVIRDAADLVVSSAGVTLGPEGRPVIMSKSYGGPEVTKDGYKVMNQLK
CCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCCCCCCHHHHHHHHHCC
PEDEKVAKIVELLNQATSQANEKAGDGTTTATILVGNMIKNAHKHIAAQRSRMKLKSGMK
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RARDEVVKYIQSVAKKINSEEEIAQVGSISANGNSEIGGKIAEAMNKVGKEGVITVEEGK
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
GLDEFSVSVVQGMVFDRGYVSPYFITNPEKMIVEFDNPYVLLANKKLSSIQPMVPLLETI
CCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCEEEEECCCHHHCCHHHHHHHHH
VRSNRAVVIIAEDVEGEALTSLVLSKMRGSLKACAVKAPGFGDRRSEMLEDIRILTGAKT
HCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHCCHH
LVSDDLGVTVESLTVEDLGTAKSIIISKDSTTIVDGGGEKTAIEARVKQIKTQIDKTTSD
HHCCCCCCEEEEEEHHHCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCH
YDKEKLQERLAKLAGGVAVLKVGGATEVEVKERKDRVEDALHATRAAVEEGIVPGGGATL
HHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LSAIAVLEKLSSDDDDEQAGINIVKTALKAPISQIVENAGEDASVITYNLLESKDPNRIF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCHHC
DARELKYVDAFKAGIIDPAKVVRVALESAVSVASVLVTTEALIVDLPTKDNGSSSMMPGG
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCC
GMGGMGGF
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9230387; 9687446; 9620368 [H]