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The map label for this gene is priA

Identifier: 88608231

GI number: 88608231

Start: 174366

End: 176495

Strand: Direct

Name: priA

Synonym: NSE_0206

Alternate gene names: 88608231

Gene position: 174366-176495 (Clockwise)

Preceding gene: 88608861

Following gene: 88608562

Centisome position: 20.3

GC content: 40.09

Gene sequence:

>2130_bases
ATGTACCTGAATGTCGTGCTGCCGCTCCCATTGGATAGTGGGTTTCTGTACTCTTCTGGACATTTTGATGTGTATGTGGG
GAGTCTTGTTAAAGTTGATTTCCGTGGGAGAAAAATAACAGGGCTCGTAACATCAGTGGTACCAGAGAGTGAACATGAAT
ACATTATAAAAGAAATCCTGGCTGTAGAGCAGGTAAGGCCTTTTTCGTCTGAGTATGTGCAATTTCTCTCTTGGATTGCA
TATTACAATTTCACCAAGCTTGGTTTGGTGCTGCGTTCCGCACTGGTTAAGGATATTCCTGATAGTGCTGCATATATTTT
GACTAACAATAATATTGAAAGATCTTGTTTAACCCCAGCAGTGTGCCGTGTGCTCGCGTTATTTAGGGGAAGTCAGTATA
TCACCTCGGATACGCTGGATGCGGAAAAGATCCAAAGGAAAACAGTGCAGAAACTTATCGATATTGGTGCAATAGAGCGT
ATTCATGCATCAGTTAGTGAGATAAAAGCTCTACCGGAGGAGCTAAAAAAACTCAAGTTCGAGCTTTCAGATGAGCAAAG
TACTGCTAGAGAAAAAATCTGTATGGAGGGGTTTACTGTATACCTTTTGGATGGTGTTACAGGTTCGGGTAAGACATTAG
TGTATCTTTCGGCTGCGCTTGAGATTCTACAGCGGGATGCAGCTGCGCAAGTTATAATGCTTTTTTCCGAGATAGCTCTT
GCTTCTTACATTTTTGAGCGAATCAAGAAAAGTTTTCTCGGGATGGTTGAAATTGTAGAATGGCATTCAGATCTCTCTCC
TGCACGTAGGAGAAAGAACTGGCACAAAGTCGTGACAAATAAAGCCAGACTGATTATTGGAGCAAGGTCAGCAATCTTAC
TTCCAATACCTAATTTACAAATGATTGTTGTAGACGAGGAACACGATCAATCCTTCAAACAGGATCAAGGAGATTTTACA
TATAATGCCAGGGATCTGGCCATAATGAGGGGTAAGTTATTCAATATTCCAGTGATTCTATCATCAGCAACACCATCTTT
GGAAAGCTATTACAATGTGCAAAAAAAAGGGTTTGTCCACCTTACATTGGAGCGCAGATTTGGCGGAGCTCAGCTTCCAG
ATGTGAGGGTGGTTGATATGCGTAAAGCTCAAAAAGTGAGTCAGCATATTTCTCATGAGTTATATGAAGAATTGAACTGT
ACTTTTGATTCTGGTATGCAGAGCCTTTTGTTTCTCAACAAACGAGGCTACGCATCGATAATAATATGTTCGAGTTGTGG
TCATAGAATGAAGTGTCCCGACTGTATTGCATGGCTAGTTGAACATAAACAGAACAACACACTTTTATGTCACTTGTGTG
GTTTCAAACGCCCCATGATAACAAAGTGTGAGGAATGTTCGTCGGATAAAATAATATCATTCGGTCCCGGGGTGGAGAAA
ATTGCTGAAGAAATTGGTGAACTCTTTCCAAGTAAGGAAGTGCTTATCATGAGTAGTGATACAAAGATTACTGAAAACAT
CCAAAAAATAGAAAAGGGTGATGTTGATATAGTGATCGGTACGCAGATCATTGCTAAGGGTTATGATTTTCCTAGACTTA
CTCTTTTGGCGATCATTGACAGCGATGTTTCCATGCATACAGGTGATTTGCGTAACTCGGAAAAGAGTTTTCAGATATTG
CAGCAAGCTATTGGACGTGTCGGTAGGAGAAAGGTGTATCCCGGTAGGGTGGTGCTACAGAGTTATTCTACAGATAGTGT
CGTAGTAGAGGCTTTAAAAAACAATGACAGAGAAACTTTCTACAAAGAAGAGCTTAAAATGAGATTAGCTGAGGAAATGC
CTCCTTTTAGTAGGCTTATTAGCATTATTATTTCTGGGCTGATAGAAAGGGAAGTCATCGCATACGCAAGAAAAATAGCA
AAAACTATTATTCCGATAAGGGAGATAAACGTTTTCGGACCTTGCCCTGCTGCAATAGGGATGAGAAAAAAACGATTTAG
ATATAGAATTCTTATTGTTGTCCCTAAAAATTTTTTCTCTCGTAATATGTTAACAGATTTGCTTACCCAATTTACCGCGG
AGAAAAAAGTAGAAGTGCAAGTTGATACAGATCCCCTGTGCTTTTTGTAA

Upstream 100 bases:

>100_bases
TTCTGGGGAAGAAATTTTGTTCGTACCTTAGTAAATGTATGCGGCTATCTAAACGGATTTCTCGAATGTTACTATTGTTT
GCACTAGTTTCTGTATCGAG

Downstream 100 bases:

>100_bases
GTTCTATATGGTAAAATGTTTACATGGATTGCAAAATTAATTATCCTAAAAGGGGCCTGGTTTTTGGTGCTAGCGTTTTT
TACTTGTGGGCCAGGTGGTG

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MYLNVVLPLPLDSGFLYSSGHFDVYVGSLVKVDFRGRKITGLVTSVVPESEHEYIIKEILAVEQVRPFSSEYVQFLSWIA
YYNFTKLGLVLRSALVKDIPDSAAYILTNNNIERSCLTPAVCRVLALFRGSQYITSDTLDAEKIQRKTVQKLIDIGAIER
IHASVSEIKALPEELKKLKFELSDEQSTAREKICMEGFTVYLLDGVTGSGKTLVYLSAALEILQRDAAAQVIMLFSEIAL
ASYIFERIKKSFLGMVEIVEWHSDLSPARRRKNWHKVVTNKARLIIGARSAILLPIPNLQMIVVDEEHDQSFKQDQGDFT
YNARDLAIMRGKLFNIPVILSSATPSLESYYNVQKKGFVHLTLERRFGGAQLPDVRVVDMRKAQKVSQHISHELYEELNC
TFDSGMQSLLFLNKRGYASIIICSSCGHRMKCPDCIAWLVEHKQNNTLLCHLCGFKRPMITKCEECSSDKIISFGPGVEK
IAEEIGELFPSKEVLIMSSDTKITENIQKIEKGDVDIVIGTQIIAKGYDFPRLTLLAIIDSDVSMHTGDLRNSEKSFQIL
QQAIGRVGRRKVYPGRVVLQSYSTDSVVVEALKNNDRETFYKEELKMRLAEEMPPFSRLISIIISGLIEREVIAYARKIA
KTIIPIREINVFGPCPAAIGMRKKRFRYRILIVVPKNFFSRNMLTDLLTQFTAEKKVEVQVDTDPLCFL

Sequences:

>Translated_709_residues
MYLNVVLPLPLDSGFLYSSGHFDVYVGSLVKVDFRGRKITGLVTSVVPESEHEYIIKEILAVEQVRPFSSEYVQFLSWIA
YYNFTKLGLVLRSALVKDIPDSAAYILTNNNIERSCLTPAVCRVLALFRGSQYITSDTLDAEKIQRKTVQKLIDIGAIER
IHASVSEIKALPEELKKLKFELSDEQSTAREKICMEGFTVYLLDGVTGSGKTLVYLSAALEILQRDAAAQVIMLFSEIAL
ASYIFERIKKSFLGMVEIVEWHSDLSPARRRKNWHKVVTNKARLIIGARSAILLPIPNLQMIVVDEEHDQSFKQDQGDFT
YNARDLAIMRGKLFNIPVILSSATPSLESYYNVQKKGFVHLTLERRFGGAQLPDVRVVDMRKAQKVSQHISHELYEELNC
TFDSGMQSLLFLNKRGYASIIICSSCGHRMKCPDCIAWLVEHKQNNTLLCHLCGFKRPMITKCEECSSDKIISFGPGVEK
IAEEIGELFPSKEVLIMSSDTKITENIQKIEKGDVDIVIGTQIIAKGYDFPRLTLLAIIDSDVSMHTGDLRNSEKSFQIL
QQAIGRVGRRKVYPGRVVLQSYSTDSVVVEALKNNDRETFYKEELKMRLAEEMPPFSRLISIIISGLIEREVIAYARKIA
KTIIPIREINVFGPCPAAIGMRKKRFRYRILIVVPKNFFSRNMLTDLLTQFTAEKKVEVQVDTDPLCFL
>Mature_709_residues
MYLNVVLPLPLDSGFLYSSGHFDVYVGSLVKVDFRGRKITGLVTSVVPESEHEYIIKEILAVEQVRPFSSEYVQFLSWIA
YYNFTKLGLVLRSALVKDIPDSAAYILTNNNIERSCLTPAVCRVLALFRGSQYITSDTLDAEKIQRKTVQKLIDIGAIER
IHASVSEIKALPEELKKLKFELSDEQSTAREKICMEGFTVYLLDGVTGSGKTLVYLSAALEILQRDAAAQVIMLFSEIAL
ASYIFERIKKSFLGMVEIVEWHSDLSPARRRKNWHKVVTNKARLIIGARSAILLPIPNLQMIVVDEEHDQSFKQDQGDFT
YNARDLAIMRGKLFNIPVILSSATPSLESYYNVQKKGFVHLTLERRFGGAQLPDVRVVDMRKAQKVSQHISHELYEELNC
TFDSGMQSLLFLNKRGYASIIICSSCGHRMKCPDCIAWLVEHKQNNTLLCHLCGFKRPMITKCEECSSDKIISFGPGVEK
IAEEIGELFPSKEVLIMSSDTKITENIQKIEKGDVDIVIGTQIIAKGYDFPRLTLLAIIDSDVSMHTGDLRNSEKSFQIL
QQAIGRVGRRKVYPGRVVLQSYSTDSVVVEALKNNDRETFYKEELKMRLAEEMPPFSRLISIIISGLIEREVIAYARKIA
KTIIPIREINVFGPCPAAIGMRKKRFRYRILIVVPKNFFSRNMLTDLLTQFTAEKKVEVQVDTDPLCFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=742, Percent_Identity=30.4582210242588, Blast_Score=316, Evalue=4e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 80316; Mature: 80316

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLNVVLPLPLDSGFLYSSGHFDVYVGSLVKVDFRGRKITGLVTSVVPESEHEYIIKEIL
CEEEEEEECCCCCCCEEECCCCEEEECCEEEEEECCCEEHHHHHHHCCCCCHHHHHHHHH
AVEQVRPFSSEYVQFLSWIAYYNFTKLGLVLRSALVKDIPDSAAYILTNNNIERSCLTPA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCHH
VCRVLALFRGSQYITSDTLDAEKIQRKTVQKLIDIGAIERIHASVSEIKALPEELKKLKF
HHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELSDEQSTAREKICMEGFTVYLLDGVTGSGKTLVYLSAALEILQRDAAAQVIMLFSEIAL
HCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ASYIFERIKKSFLGMVEIVEWHSDLSPARRRKNWHKVVTNKARLIIGARSAILLPIPNLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHCCCCEEEEEECCEEEEECCCEE
MIVVDEEHDQSFKQDQGDFTYNARDLAIMRGKLFNIPVILSSATPSLESYYNVQKKGFVH
EEEEECCCCHHHHCCCCCEEECCHHHHEECCCEECCEEEEECCCCCHHHHHHHCCCCEEE
LTLERRFGGAQLPDVRVVDMRKAQKVSQHISHELYEELNCTFDSGMQSLLFLNKRGYASI
EEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEE
IICSSCGHRMKCPDCIAWLVEHKQNNTLLCHLCGFKRPMITKCEECSSDKIISFGPGVEK
EEECCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEECCCCHHH
IAEEIGELFPSKEVLIMSSDTKITENIQKIEKGDVDIVIGTQIIAKGYDFPRLTLLAIID
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCEEEEEEEC
SDVSMHTGDLRNSEKSFQILQQAIGRVGRRKVYPGRVVLQSYSTDSVVVEALKNNDRETF
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHH
YKEELKMRLAEEMPPFSRLISIIISGLIEREVIAYARKIAKTIIPIREINVFGPCPAAIG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHC
MRKKRFRYRILIVVPKNFFSRNMLTDLLTQFTAEKKVEVQVDTDPLCFL
CHHHCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MYLNVVLPLPLDSGFLYSSGHFDVYVGSLVKVDFRGRKITGLVTSVVPESEHEYIIKEIL
CEEEEEEECCCCCCCEEECCCCEEEECCEEEEEECCCEEHHHHHHHCCCCCHHHHHHHHH
AVEQVRPFSSEYVQFLSWIAYYNFTKLGLVLRSALVKDIPDSAAYILTNNNIERSCLTPA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCCHH
VCRVLALFRGSQYITSDTLDAEKIQRKTVQKLIDIGAIERIHASVSEIKALPEELKKLKF
HHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELSDEQSTAREKICMEGFTVYLLDGVTGSGKTLVYLSAALEILQRDAAAQVIMLFSEIAL
HCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ASYIFERIKKSFLGMVEIVEWHSDLSPARRRKNWHKVVTNKARLIIGARSAILLPIPNLQ
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHCCCCEEEEEECCEEEEECCCEE
MIVVDEEHDQSFKQDQGDFTYNARDLAIMRGKLFNIPVILSSATPSLESYYNVQKKGFVH
EEEEECCCCHHHHCCCCCEEECCHHHHEECCCEECCEEEEECCCCCHHHHHHHCCCCEEE
LTLERRFGGAQLPDVRVVDMRKAQKVSQHISHELYEELNCTFDSGMQSLLFLNKRGYASI
EEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEE
IICSSCGHRMKCPDCIAWLVEHKQNNTLLCHLCGFKRPMITKCEECSSDKIISFGPGVEK
EEECCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEECCCCHHH
IAEEIGELFPSKEVLIMSSDTKITENIQKIEKGDVDIVIGTQIIAKGYDFPRLTLLAIID
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCHHHHCCCCCCCEEEEEEEC
SDVSMHTGDLRNSEKSFQILQQAIGRVGRRKVYPGRVVLQSYSTDSVVVEALKNNDRETF
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHH
YKEELKMRLAEEMPPFSRLISIIISGLIEREVIAYARKIAKTIIPIREINVFGPCPAAIG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCCHHHHC
MRKKRFRYRILIVVPKNFFSRNMLTDLLTQFTAEKKVEVQVDTDPLCFL
CHHHCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]