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The map label for this gene is gyrB

Identifier: 88608222

GI number: 88608222

Start: 642578

End: 644929

Strand: Reverse

Name: gyrB

Synonym: NSE_0721

Alternate gene names: 88608222

Gene position: 644929-642578 (Counterclockwise)

Preceding gene: 88608272

Following gene: 88608108

Centisome position: 75.08

GC content: 43.15

Gene sequence:

>2352_bases
TTGAGTAACACGGATTATACAGCAGATTCGATAAAGGTATTGAAGGGTCTTGAAGCTGTTCGGAAAAGACCCGGAATGTA
CATCGGAGACACAGATGATGGGTCAGGGTTGCATCAAATGGTGTATGAAGTTGTTGATAATTCCGTAGATGAATCCCTTG
CGGGCTATTGTACTGCTATAGAGGTTGTCATTGATGTTGATGGGTCGATCTCTGTTGAGGACAACGGGCGTGGTATGCCC
ACAGATATGCATGGAGAAGGTGTGTCTGCAGCCGAGGTGATTATGACGCAACTGCATGCTGGCGGGAAATTCGATCAGAG
CAGCTATAAAGTATCTGGTGGTCTTCACGGTGTTGGTGTGTCGGTAGTAAATGCCCTTTCTGATTGGCTTAAGTTGACGA
TTTACAGAGATGGGAAAGTTCACTTCGCGGAATTTGAAAATGGTGAAACAGTACGCTCTTTGTCTGTTACCGGGGAGTGT
GGTGATAAAACTGGGACATTAGTTCATTTTCTTCCATCGAGAAGTACATTCAAAAACACGACAGAGGTTAGCTTTAGCAC
TCTTGAGAATAGATTTAGAGAGCTGTCGTTCCTAAATCCTGGACTAAGGATTTCTCTACTTGATATGAGGAAAGGTAGTA
GTACAGGAAAGGTAGTTTTTTTCTCTTCGGGTGGTACTGAAGAATTTGTTGCTTATTTGGACCGAAGCAAGCAACCTATT
CACTCGACTCCAATCAGAGTTGTGGGTGGAGTTGATTCCATAGCGATAGACGTATCAATGCAGTGGAATGATTCTTATTA
TGAAAATGTCCTCTGTTTCACAAACAATATAAAGCAAAAAGATGGTGGCACACACCTTGCTGCGCTTAGGAGCGCGATGA
CAAGAGTTATGCATAACTATATTGTCAAGGAGAACCTGATTAAGAAGGACAAAGTTGTTGTGTCCGGCGAGGATGTAAGG
GAGGGTCTGAGCGCAATTCTCTCTGTTAAGCTTCCGGACCCAAAATTTTCCTCTCAAACTAAGGAGAAACTCATTAGTTC
TGAGGTCAAGCCTGTAATAGAAAAAATAGTCAGTGATGCGCTGGCAAGTTGGCTTGAGGAGAATCCCAGTTCTGCAAGGG
TGATCGCAAGGAAGGTAGTCGAGTCCGCTCTAGCAAGAGAGGCAGCAAAAAAAGCGCGGGATCTCACTCGTAGAAAAGGC
GGGCTGGAAATAACAACTCTTCCGGGAAAGCTTGCCGATTGTCAAGAAAAATCTCCTGAGCTTTCAGAATTGTTGATTGT
TGAGGGTGATTCAGCAGGAGGCTCAGCAAAACAAGGTAGAGATAGAAAAACTCAAGCTGTTTTACCATTAAGAGGAAAAA
TTCTTAATGTTGAGAAAACGCGGTTTCATAAGGTTATAGGTTCAGCGGAGATAGGAAATTTGATAGCTGCTTTGGGCACT
AGCATAGGAGAAGAGGAGTTTAATATTGATAAAATCAGATACCACAAGATCATCATCATGACAGATGCTGATGTTGATGG
TCTGCATATCAGAACGCTTTTGCTGACGTTTTTTTTTCGTTATGCGCGTCCGATTGTTGAGCGTGGGTATTTGTATATTG
CTCAAACGCCGTTGTATAAGGTGATGAGGAAGGGCGCAGATGTTTATCTCCGTGATGATGCAGCTCTTGAGGAATACCTG
CTGGACCGTATTGTGAAAGGTAAAGAATTAGAAGGTGCTAATGGTAATGTTTATACTGGTCAATCATTGAAGCGCGTTGT
GCAGCAATGTTTAGTTCTCTCGAAAATTCTTGAAAAGTTTGACTATAGGATACCTTTGGAGATTTTGGAGGTGGTTTTTC
TGTGTGGTGAAGAGGCCGACCCTCATCTTCTTGCTGAAAGGGTGGCTCTTATCTCTTTTGGCGCATGGACTGTTGTTCGT
GAAGATAGTAGCTTGTTTTTTGAGCGGACTTTCCAGGGGTTGAGAAACCGATATGAGTACTCTCCACTTTTTGTTCCTGA
AGATCTTGAGAAGATGGCTAAAATCCGTGAGGCTATTAGTGATATTTTTTACCCTGGTTTTGCAAGATTCAATAATGGAG
AGTACCACTCTATTCTTGCTTTCATTAGGGATGCGGATTCAGTGGCCAGAGCTGGCATGGTCTTGCAAAGGTTTAAAGGT
CTAGGTGAAATGAATCCAGAACAGTTATGGTCTACTACCCTCGATCCTTCCAACAGAACAATTTTTAAAGTTACTATAGA
AGAGGCTGCCGTAGCCGATCAAATGTGCTCCATGCTAATGGGGGACGCCGTTGGCCCCCGTAGGGACTTTATTGTCAATA
ATGCCCTAAATGCAAGTAATATAGATGTGTGA

Upstream 100 bases:

>100_bases
CTATTGAAAGTGGCTGTGTGGATTTCTTATGGCCTAAAATGTTTCACTATAACGTTCTTTCTGATACAATCCCTGTGGCT
TTTACACTATTTCGACCACA

Downstream 100 bases:

>100_bases
TCTATCTCTCGTTATTTTTGGGTTACTATGTTAACCCTAAGAGCGCCGGCAAAGATTAATCTTTTTCTGCTTATCACAGG
AAGGAATGAATCCGGGTTTC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 783; Mature: 782

Protein sequence:

>783_residues
MSNTDYTADSIKVLKGLEAVRKRPGMYIGDTDDGSGLHQMVYEVVDNSVDESLAGYCTAIEVVIDVDGSISVEDNGRGMP
TDMHGEGVSAAEVIMTQLHAGGKFDQSSYKVSGGLHGVGVSVVNALSDWLKLTIYRDGKVHFAEFENGETVRSLSVTGEC
GDKTGTLVHFLPSRSTFKNTTEVSFSTLENRFRELSFLNPGLRISLLDMRKGSSTGKVVFFSSGGTEEFVAYLDRSKQPI
HSTPIRVVGGVDSIAIDVSMQWNDSYYENVLCFTNNIKQKDGGTHLAALRSAMTRVMHNYIVKENLIKKDKVVVSGEDVR
EGLSAILSVKLPDPKFSSQTKEKLISSEVKPVIEKIVSDALASWLEENPSSARVIARKVVESALAREAAKKARDLTRRKG
GLEITTLPGKLADCQEKSPELSELLIVEGDSAGGSAKQGRDRKTQAVLPLRGKILNVEKTRFHKVIGSAEIGNLIAALGT
SIGEEEFNIDKIRYHKIIIMTDADVDGLHIRTLLLTFFFRYARPIVERGYLYIAQTPLYKVMRKGADVYLRDDAALEEYL
LDRIVKGKELEGANGNVYTGQSLKRVVQQCLVLSKILEKFDYRIPLEILEVVFLCGEEADPHLLAERVALISFGAWTVVR
EDSSLFFERTFQGLRNRYEYSPLFVPEDLEKMAKIREAISDIFYPGFARFNNGEYHSILAFIRDADSVARAGMVLQRFKG
LGEMNPEQLWSTTLDPSNRTIFKVTIEEAAVADQMCSMLMGDAVGPRRDFIVNNALNASNIDV

Sequences:

>Translated_783_residues
MSNTDYTADSIKVLKGLEAVRKRPGMYIGDTDDGSGLHQMVYEVVDNSVDESLAGYCTAIEVVIDVDGSISVEDNGRGMP
TDMHGEGVSAAEVIMTQLHAGGKFDQSSYKVSGGLHGVGVSVVNALSDWLKLTIYRDGKVHFAEFENGETVRSLSVTGEC
GDKTGTLVHFLPSRSTFKNTTEVSFSTLENRFRELSFLNPGLRISLLDMRKGSSTGKVVFFSSGGTEEFVAYLDRSKQPI
HSTPIRVVGGVDSIAIDVSMQWNDSYYENVLCFTNNIKQKDGGTHLAALRSAMTRVMHNYIVKENLIKKDKVVVSGEDVR
EGLSAILSVKLPDPKFSSQTKEKLISSEVKPVIEKIVSDALASWLEENPSSARVIARKVVESALAREAAKKARDLTRRKG
GLEITTLPGKLADCQEKSPELSELLIVEGDSAGGSAKQGRDRKTQAVLPLRGKILNVEKTRFHKVIGSAEIGNLIAALGT
SIGEEEFNIDKIRYHKIIIMTDADVDGLHIRTLLLTFFFRYARPIVERGYLYIAQTPLYKVMRKGADVYLRDDAALEEYL
LDRIVKGKELEGANGNVYTGQSLKRVVQQCLVLSKILEKFDYRIPLEILEVVFLCGEEADPHLLAERVALISFGAWTVVR
EDSSLFFERTFQGLRNRYEYSPLFVPEDLEKMAKIREAISDIFYPGFARFNNGEYHSILAFIRDADSVARAGMVLQRFKG
LGEMNPEQLWSTTLDPSNRTIFKVTIEEAAVADQMCSMLMGDAVGPRRDFIVNNALNASNIDV
>Mature_782_residues
SNTDYTADSIKVLKGLEAVRKRPGMYIGDTDDGSGLHQMVYEVVDNSVDESLAGYCTAIEVVIDVDGSISVEDNGRGMPT
DMHGEGVSAAEVIMTQLHAGGKFDQSSYKVSGGLHGVGVSVVNALSDWLKLTIYRDGKVHFAEFENGETVRSLSVTGECG
DKTGTLVHFLPSRSTFKNTTEVSFSTLENRFRELSFLNPGLRISLLDMRKGSSTGKVVFFSSGGTEEFVAYLDRSKQPIH
STPIRVVGGVDSIAIDVSMQWNDSYYENVLCFTNNIKQKDGGTHLAALRSAMTRVMHNYIVKENLIKKDKVVVSGEDVRE
GLSAILSVKLPDPKFSSQTKEKLISSEVKPVIEKIVSDALASWLEENPSSARVIARKVVESALAREAAKKARDLTRRKGG
LEITTLPGKLADCQEKSPELSELLIVEGDSAGGSAKQGRDRKTQAVLPLRGKILNVEKTRFHKVIGSAEIGNLIAALGTS
IGEEEFNIDKIRYHKIIIMTDADVDGLHIRTLLLTFFFRYARPIVERGYLYIAQTPLYKVMRKGADVYLRDDAALEEYLL
DRIVKGKELEGANGNVYTGQSLKRVVQQCLVLSKILEKFDYRIPLEILEVVFLCGEEADPHLLAERVALISFGAWTVVRE
DSSLFFERTFQGLRNRYEYSPLFVPEDLEKMAKIREAISDIFYPGFARFNNGEYHSILAFIRDADSVARAGMVLQRFKGL
GEMNPEQLWSTTLDPSNRTIFKVTIEEAAVADQMCSMLMGDAVGPRRDFIVNNALNASNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=595, Percent_Identity=24.8739495798319, Blast_Score=120, Evalue=7e-27,
Organism=Homo sapiens, GI19913406, Length=592, Percent_Identity=25.5067567567568, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI48994957, Length=818, Percent_Identity=50.7334963325183, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=575, Percent_Identity=40.3478260869565, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535065, Length=539, Percent_Identity=23.747680890538, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI212645845, Length=523, Percent_Identity=25.4302103250478, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=165, Percent_Identity=32.1212121212121, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=588, Percent_Identity=24.1496598639456, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17136538, Length=552, Percent_Identity=25.7246376811594, Blast_Score=129, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 86719; Mature: 86588

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTDYTADSIKVLKGLEAVRKRPGMYIGDTDDGSGLHQMVYEVVDNSVDESLAGYCTAI
CCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHEEE
EVVIDVDGSISVEDNGRGMPTDMHGEGVSAAEVIMTQLHAGGKFDQSSYKVSGGLHGVGV
EEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSDWLKLTIYRDGKVHFAEFENGETVRSLSVTGECGDKTGTLVHFLPSRSTFKNT
HHHHHHHHHHEEEEEECCCEEEEECCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCC
TEVSFSTLENRFRELSFLNPGLRISLLDMRKGSSTGKVVFFSSGGTEEFVAYLDRSKQPI
CHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCC
HSTPIRVVGGVDSIAIDVSMQWNDSYYENVLCFTNNIKQKDGGTHLAALRSAMTRVMHNY
CCCCEEEECCCCEEEEEEEEEECCHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
IVKENLIKKDKVVVSGEDVREGLSAILSVKLPDPKFSSQTKEKLISSEVKPVIEKIVSDA
HHHHHHHCCCCEEECCHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASWLEENPSSARVIARKVVESALAREAAKKARDLTRRKGGLEITTLPGKLADCQEKSPE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCC
LSELLIVEGDSAGGSAKQGRDRKTQAVLPLRGKILNVEKTRFHKVIGSAEIGNLIAALGT
CEEEEEEECCCCCCCCCCCCCCCCCEECCCCCEEECCHHHHHHHHHCCHHHHHHHHHHHC
SIGEEEFNIDKIRYHKIIIMTDADVDGLHIRTLLLTFFFRYARPIVERGYLYIAQTPLYK
CCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHH
VMRKGADVYLRDDAALEEYLLDRIVKGKELEGANGNVYTGQSLKRVVQQCLVLSKILEKF
HHHCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHC
DYRIPLEILEVVFLCGEEADPHLLAERVALISFGAWTVVREDSSLFFERTFQGLRNRYEY
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHHCCCC
SPLFVPEDLEKMAKIREAISDIFYPGFARFNNGEYHSILAFIRDADSVARAGMVLQRFKG
CCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
LGEMNPEQLWSTTLDPSNRTIFKVTIEEAAVADQMCSMLMGDAVGPRRDFIVNNALNASN
CCCCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCC
IDV
CCC
>Mature Secondary Structure 
SNTDYTADSIKVLKGLEAVRKRPGMYIGDTDDGSGLHQMVYEVVDNSVDESLAGYCTAI
CCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHCHHHHHHHHHHEEE
EVVIDVDGSISVEDNGRGMPTDMHGEGVSAAEVIMTQLHAGGKFDQSSYKVSGGLHGVGV
EEEEECCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSDWLKLTIYRDGKVHFAEFENGETVRSLSVTGECGDKTGTLVHFLPSRSTFKNT
HHHHHHHHHHEEEEEECCCEEEEECCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCC
TEVSFSTLENRFRELSFLNPGLRISLLDMRKGSSTGKVVFFSSGGTEEFVAYLDRSKQPI
CHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCCC
HSTPIRVVGGVDSIAIDVSMQWNDSYYENVLCFTNNIKQKDGGTHLAALRSAMTRVMHNY
CCCCEEEECCCCEEEEEEEEEECCHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
IVKENLIKKDKVVVSGEDVREGLSAILSVKLPDPKFSSQTKEKLISSEVKPVIEKIVSDA
HHHHHHHCCCCEEECCHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASWLEENPSSARVIARKVVESALAREAAKKARDLTRRKGGLEITTLPGKLADCQEKSPE
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCCCCC
LSELLIVEGDSAGGSAKQGRDRKTQAVLPLRGKILNVEKTRFHKVIGSAEIGNLIAALGT
CEEEEEEECCCCCCCCCCCCCCCCCEECCCCCEEECCHHHHHHHHHCCHHHHHHHHHHHC
SIGEEEFNIDKIRYHKIIIMTDADVDGLHIRTLLLTFFFRYARPIVERGYLYIAQTPLYK
CCCCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHH
VMRKGADVYLRDDAALEEYLLDRIVKGKELEGANGNVYTGQSLKRVVQQCLVLSKILEKF
HHHCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHC
DYRIPLEILEVVFLCGEEADPHLLAERVALISFGAWTVVREDSSLFFERTFQGLRNRYEY
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHHCCCC
SPLFVPEDLEKMAKIREAISDIFYPGFARFNNGEYHSILAFIRDADSVARAGMVLQRFKG
CCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
LGEMNPEQLWSTTLDPSNRTIFKVTIEEAAVADQMCSMLMGDAVGPRRDFIVNNALNASN
CCCCCHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCC
IDV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]