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The map label for this gene is gshB

Identifier: 88608203

GI number: 88608203

Start: 156485

End: 157459

Strand: Reverse

Name: gshB

Synonym: NSE_0186

Alternate gene names: 88608203

Gene position: 157459-156485 (Counterclockwise)

Preceding gene: 88608364

Following gene: 88608740

Centisome position: 18.33

GC content: 41.23

Gene sequence:

>975_bases
TTGTTCAGTCTAGAAATTGAGATAGACTACAGAAGATACCCGGACATTACAATGAAAGTAGGATTACAGGTGCTTGAGCT
TGACGCACCTGGAAAAAGCAGCATCTATCTACTCGAAGAAGCTCTAGAAAGAGAGTGCAGCGTCTTTATCTATCCTGTAA
AAACTCTGTGTATTTGCAGTGGTCGCGTTTTCGCTCGTGGGAAATATGTGGAGAGCTGTCATAAAGGCGCAATCACTTTA
TCTAAGGAAGAAATAGAAGTGAATTTAGATACATTAGATCTCCTCCTAATGAGAAACGACCCCCCGTTCAACATGGACTA
CATAACTGCGACATACATCTTGGAAAGAAGTGGCGCTTTGGTCGTAAACAATCCAGTAGCAGTGCGCAATTTTCCTGAAA
AATTCGTGGATTACTATGAGGATACGTTTCCAACTCTCATATCACGCGATATATGGAATATCAAACTCTTCATCGAACAA
TATAGAGAAGTGGTAGTGAAGCCGCTTTACGCTTTTGCTGGAATCGATGTAACAAAAATATCGAATGCAAACGAGAGTAC
TCTTGCCCGTGTTCAGAGTTTAATTGAAAAAACTGGAACTCCGGTAGTGATACAAAAATACGATGAATCTGTGATTGAGC
AAGGTGATAAACGAGTTGTAGTACTCAATGGAGGCTTGCTGGGCTGTCTAAAGCGACATAATAAAAACAGTTTCATCACA
AATTTACGCCAGGGAGGTGAATTCTTCTCATGTACCCTTACTCCAATAGAAGAAGAAAGATGCTACAAGATTGCCTCAGA
CCTAAAAGAATCAGGTATCATACTTGCAGGAATAGATCTCGTTGCAGGGAGGATAACCGAAATCAATGTCACTTCAACAG
CCTGCATAGCGGAACTGAATGAACTCTACCACATAAACACTGCCGCTAGGTGCTTTGATGTCTTTGAGGAAATGGTGTGG
TCCCATAAGCGTTAA

Upstream 100 bases:

>100_bases
TAAGCCAGGAGATGTATCATTAAGACCTGTCGAGTTAAAAAATTACATGTGTAAAATCTCACTATCATATACACATTCGC
AATGGAAATACACAAGCTCT

Downstream 100 bases:

>100_bases
GCAAGCAGACCTCCTCTGCATAAAATCAGCAGAAAATCAATTTCTTTTTCGAATTCTAGACAAAAAACAGTAAAAGTGCT
AACATTCGGCTATGATCTAT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MFSLEIEIDYRRYPDITMKVGLQVLELDAPGKSSIYLLEEALERECSVFIYPVKTLCICSGRVFARGKYVESCHKGAITL
SKEEIEVNLDTLDLLLMRNDPPFNMDYITATYILERSGALVVNNPVAVRNFPEKFVDYYEDTFPTLISRDIWNIKLFIEQ
YREVVVKPLYAFAGIDVTKISNANESTLARVQSLIEKTGTPVVIQKYDESVIEQGDKRVVVLNGGLLGCLKRHNKNSFIT
NLRQGGEFFSCTLTPIEEERCYKIASDLKESGIILAGIDLVAGRITEINVTSTACIAELNELYHINTAARCFDVFEEMVW
SHKR

Sequences:

>Translated_324_residues
MFSLEIEIDYRRYPDITMKVGLQVLELDAPGKSSIYLLEEALERECSVFIYPVKTLCICSGRVFARGKYVESCHKGAITL
SKEEIEVNLDTLDLLLMRNDPPFNMDYITATYILERSGALVVNNPVAVRNFPEKFVDYYEDTFPTLISRDIWNIKLFIEQ
YREVVVKPLYAFAGIDVTKISNANESTLARVQSLIEKTGTPVVIQKYDESVIEQGDKRVVVLNGGLLGCLKRHNKNSFIT
NLRQGGEFFSCTLTPIEEERCYKIASDLKESGIILAGIDLVAGRITEINVTSTACIAELNELYHINTAARCFDVFEEMVW
SHKR
>Mature_324_residues
MFSLEIEIDYRRYPDITMKVGLQVLELDAPGKSSIYLLEEALERECSVFIYPVKTLCICSGRVFARGKYVESCHKGAITL
SKEEIEVNLDTLDLLLMRNDPPFNMDYITATYILERSGALVVNNPVAVRNFPEKFVDYYEDTFPTLISRDIWNIKLFIEQ
YREVVVKPLYAFAGIDVTKISNANESTLARVQSLIEKTGTPVVIQKYDESVIEQGDKRVVVLNGGLLGCLKRHNKNSFIT
NLRQGGEFFSCTLTPIEEERCYKIASDLKESGIILAGIDLVAGRITEINVTSTACIAELNELYHINTAARCFDVFEEMVW
SHKR

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=293, Percent_Identity=31.740614334471, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 36769; Mature: 36769

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSLEIEIDYRRYPDITMKVGLQVLELDAPGKSSIYLLEEALERECSVFIYPVKTLCICS
CEEEEEEECCCCCCCEEEECCCEEEEECCCCCCCEEHHHHHHHCCCCEEEEEHHHHHHCC
GRVFARGKYVESCHKGAITLSKEEIEVNLDTLDLLLMRNDPPFNMDYITATYILERSGAL
CCEEECCHHHHHHCCCCEEEEHHHEEEEECEEEEEEEECCCCCCCHHEEEEEEEECCCEE
VVNNPVAVRNFPEKFVDYYEDTFPTLISRDIWNIKLFIEQYREVVVKPLYAFAGIDVTKI
EEECCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
SNANESTLARVQSLIEKTGTPVVIQKYDESVIEQGDKRVVVLNGGLLGCLKRHNKNSFIT
CCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCEEEEEECCCEEHHHHCCCCHHHH
NLRQGGEFFSCTLTPIEEERCYKIASDLKESGIILAGIDLVAGRITEINVTSTACIAELN
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEEHHHHHCCEEEEECCHHHHHHHHH
ELYHINTAARCFDVFEEMVWSHKR
HHHCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFSLEIEIDYRRYPDITMKVGLQVLELDAPGKSSIYLLEEALERECSVFIYPVKTLCICS
CEEEEEEECCCCCCCEEEECCCEEEEECCCCCCCEEHHHHHHHCCCCEEEEEHHHHHHCC
GRVFARGKYVESCHKGAITLSKEEIEVNLDTLDLLLMRNDPPFNMDYITATYILERSGAL
CCEEECCHHHHHHCCCCEEEEHHHEEEEECEEEEEEEECCCCCCCHHEEEEEEEECCCEE
VVNNPVAVRNFPEKFVDYYEDTFPTLISRDIWNIKLFIEQYREVVVKPLYAFAGIDVTKI
EEECCEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
SNANESTLARVQSLIEKTGTPVVIQKYDESVIEQGDKRVVVLNGGLLGCLKRHNKNSFIT
CCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCEEEEEECCCEEHHHHCCCCHHHH
NLRQGGEFFSCTLTPIEEERCYKIASDLKESGIILAGIDLVAGRITEINVTSTACIAELN
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEEHHHHHCCEEEEECCHHHHHHHHH
ELYHINTAARCFDVFEEMVWSHKR
HHHCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]