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The map label for this gene is birA [C]

Identifier: 88608177

GI number: 88608177

Start: 614856

End: 615536

Strand: Direct

Name: birA [C]

Synonym: NSE_0693

Alternate gene names: 88608177

Gene position: 614856-615536 (Clockwise)

Preceding gene: 88608865

Following gene: 88607980

Centisome position: 71.58

GC content: 36.86

Gene sequence:

>681_bases
ATGTTTTCGCGCCCTCTTAACCTAATACGCCTCAAGTCTCCAGAAAATACGAATGATGCTGTAAAAGACTTCATCTCGAA
AGACAATTGTACACTCCTCATTGGAGAAAAACCGCTTTTTGGAAAAACAAGAAATGGGAAAAGAGATTGGAAAATCGTGG
AAGGTAATCTTTTCATGAGTATCGGTCTATTCAACGAAGATGTAAAAACTGGAGAAATCGTTCTTAAGGTGGTTGTTGCA
GTAGGTGAGCTACTCTCAAACTGGATAAAGGATATTCAATATAAGTGGCCTAATGATATCCTAGTCAATGGAAAGAAGAT
TTGCGGAATATTACTGGAATATAATTCTGGCAAATTAGTTATAGGGATAGGGATGAATGTATTACATTCACCATATGAAT
GGACTACCTGTTTAGATAAATACCACCAGATTTCACTCGATGAACTTTCACTGCAGATCCTAGCCTCACTCAGAGAACAC
GAGAGCAAACCAATTGAACAAATCATTAAAATATTGAGAAGCAAGGCATTCTTACTGGGAAAAGAAATCGACTTTAATAC
AAACGGAAAATTAGAAAGAGGTCTTTTCATTGATGTAGCACCAGATGGAGCTCTCGTGCTTGAAGATCTCTACACTAAAG
AGCAAAAAAAATTTTATTCGGGGTCTATATGTACTACGTAA

Upstream 100 bases:

>100_bases
TCGTGACTGGTCTGTTCGTTGCAGCAGCATCGGTTCGATATTAAGCTATTTTAAGGACACTCCTTAAGTATGTTCTATTC
TACTAATTTGCTCTCCTCTA

Downstream 100 bases:

>100_bases
TTACCGCATACCTTGTATACATTGGATTATTAGGCTGGTGTAGCTGGAGAATCATACAGCGATACATTGATTCTCAACGA
AAGCTGCGTGATCTTGATCA

Product: biotin--acetyl-CoA-carboxylase ligase

Products: AMP; diphosphate; [acetyl-CoA:carbon-dioxideligase (ADP-forming)] [C]

Alternate protein names: Biotin/Acetyl-CoA-Carboxylase Ligase; Biotin-(Acetyl-CoA Carboxylase) Ligase; Biotin-Protein Ligase; Biotin-[Acetyl-Coa-Carboxylase] Ligase; Biotin-(Acetyl-CoA-Carboxylase) Ligase; Biotin-[Acetyl-CoA-Carboxylase] Ligase BirA; Biotin Protein Ligase; Biotin-(Acetyl-COA-Carboxylase) Synthetase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MFSRPLNLIRLKSPENTNDAVKDFISKDNCTLLIGEKPLFGKTRNGKRDWKIVEGNLFMSIGLFNEDVKTGEIVLKVVVA
VGELLSNWIKDIQYKWPNDILVNGKKICGILLEYNSGKLVIGIGMNVLHSPYEWTTCLDKYHQISLDELSLQILASLREH
ESKPIEQIIKILRSKAFLLGKEIDFNTNGKLERGLFIDVAPDGALVLEDLYTKEQKKFYSGSICTT

Sequences:

>Translated_226_residues
MFSRPLNLIRLKSPENTNDAVKDFISKDNCTLLIGEKPLFGKTRNGKRDWKIVEGNLFMSIGLFNEDVKTGEIVLKVVVA
VGELLSNWIKDIQYKWPNDILVNGKKICGILLEYNSGKLVIGIGMNVLHSPYEWTTCLDKYHQISLDELSLQILASLREH
ESKPIEQIIKILRSKAFLLGKEIDFNTNGKLERGLFIDVAPDGALVLEDLYTKEQKKFYSGSICTT
>Mature_226_residues
MFSRPLNLIRLKSPENTNDAVKDFISKDNCTLLIGEKPLFGKTRNGKRDWKIVEGNLFMSIGLFNEDVKTGEIVLKVVVA
VGELLSNWIKDIQYKWPNDILVNGKKICGILLEYNSGKLVIGIGMNVLHSPYEWTTCLDKYHQISLDELSLQILASLREH
ESKPIEQIIKILRSKAFLLGKEIDFNTNGKLERGLFIDVAPDGALVLEDLYTKEQKKFYSGSICTT

Specific function: Bira Acts Both As A Biotin-Operon Repressor And As The Enzyme That Synthesizes The Corepressor, Acetyl-CoA:Carbon- Dioxide Ligase. This Protein Also Activates Biotin To Form Biotinyl-5'-Adenylate And Transfers The Biotin Moiety To Biotin-Accepting Protei

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.4.15 [C]

Molecular weight: Translated: 25605; Mature: 25605

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRPLNLIRLKSPENTNDAVKDFISKDNCTLLIGEKPLFGKTRNGKRDWKIVEGNLFMS
CCCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEECCEEEE
IGLFNEDVKTGEIVLKVVVAVGELLSNWIKDIQYKWPNDILVNGKKICGILLEYNSGKLV
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEEEEECCCEEE
IGIGMNVLHSPYEWTTCLDKYHQISLDELSLQILASLREHESKPIEQIIKILRSKAFLLG
EEECHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
KEIDFNTNGKLERGLFIDVAPDGALVLEDLYTKEQKKFYSGSICTT
CCCCCCCCCCEECCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFSRPLNLIRLKSPENTNDAVKDFISKDNCTLLIGEKPLFGKTRNGKRDWKIVEGNLFMS
CCCCCCCEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCEEEEECCEEEE
IGLFNEDVKTGEIVLKVVVAVGELLSNWIKDIQYKWPNDILVNGKKICGILLEYNSGKLV
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEEEEECCCEEE
IGIGMNVLHSPYEWTTCLDKYHQISLDELSLQILASLREHESKPIEQIIKILRSKAFLLG
EEECHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
KEIDFNTNGKLERGLFIDVAPDGALVLEDLYTKEQKKFYSGSICTT
CCCCCCCCCCEECCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; biotin; apo-[acetyl-CoA:carbon-dioxide ligase(ADP-forming)] [C]

Specific reaction: ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase(ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxideligase (ADP-forming)] [C]

General reaction: Amination; Carboxylic acid formation (carboxamide formation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA