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The map label for this gene is ispE

Identifier: 88608108

GI number: 88608108

Start: 641711

End: 642550

Strand: Reverse

Name: ispE

Synonym: NSE_0720

Alternate gene names: 88608108

Gene position: 642550-641711 (Counterclockwise)

Preceding gene: 88608222

Following gene: 88607986

Centisome position: 74.8

GC content: 41.9

Gene sequence:

>840_bases
ATGTTAACCCTAAGAGCGCCGGCAAAGATTAATCTTTTTCTGCTTATCACAGGAAGGAATGAATCCGGGTTTCACCTGCT
CGATAGTGTTTTCTGTTTCACGCATGATCTGTATGACGAGATAATAATTGTTCCTACGTCTGCAACTGAGAACTCAGTCA
AATTCATTGGTGCATCCCAAGCGATAGGTGCGGATAACTCGGTAAGTCGTGTTCTCAGATTTGTTAATGATTATGCAAAG
CGTTATTTCAATGTTACTCTAGTGAAGAATCTTCCTGTTGCTGCCGGTATAGGTGGTGGTTCAGCAGATGCAGCAGCGTT
GCTGAGATATTTCGGTGGGCTACACAATATCTCGGAAGTATTACTCAGTCAAGTGGCTTTGTCTGTTGGCTTTGATACGT
TGGCCTGCCTTTATAGCCATCCGTGTCACGTAACGGGAATGGGTGAAAAAATCGTTGCATTGCCAGAGTCGAAGCTTGTA
TATCCAATACTCCTAGTCTGTCCAAACTTTCCTGTCAGCTCTGCTGAAGTTTATAAAAATTTCCGCGAAAGAAGAAGTAT
TTTTTCAGCTGAGTCCAATCTTGCAGGGGATCATCAGGCGTTTGAAGATATGCTTTCTCTTCCTAATGATTTAACTGATG
CTGCTATCGAAATCGCACCAGTGATAAGCGACGTACTTAAAAGTATTAAAAATTGTAAAGGAAATAAGGTCACCAAACTA
TGTGGGAGCGGTCCAACATGTATAGGTGTTTTCGACACTGTAGAAAATCTAAGAGACGCCCACTCAGAATTAATACGTCA
TCATAAGTGGTGGGTTAAGATCGCACATATTTCGCTGTAA

Upstream 100 bases:

>100_bases
GGGGGACGCCGTTGGCCCCCGTAGGGACTTTATTGTCAATAATGCCCTAAATGCAAGTAATATAGATGTGTGATCTATCT
CTCGTTATTTTTGGGTTACT

Downstream 100 bases:

>100_bases
TTGGGAATGTTTACTCTGTTAGCACATCAGTAGTTTTTCGGTATTTCTGAGGATATTTTACGTTTAGTTGGTATTAGTCT
TGGAATCCTGATATGTAGGT

Product: putative 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MLTLRAPAKINLFLLITGRNESGFHLLDSVFCFTHDLYDEIIIVPTSATENSVKFIGASQAIGADNSVSRVLRFVNDYAK
RYFNVTLVKNLPVAAGIGGGSADAAALLRYFGGLHNISEVLLSQVALSVGFDTLACLYSHPCHVTGMGEKIVALPESKLV
YPILLVCPNFPVSSAEVYKNFRERRSIFSAESNLAGDHQAFEDMLSLPNDLTDAAIEIAPVISDVLKSIKNCKGNKVTKL
CGSGPTCIGVFDTVENLRDAHSELIRHHKWWVKIAHISL

Sequences:

>Translated_279_residues
MLTLRAPAKINLFLLITGRNESGFHLLDSVFCFTHDLYDEIIIVPTSATENSVKFIGASQAIGADNSVSRVLRFVNDYAK
RYFNVTLVKNLPVAAGIGGGSADAAALLRYFGGLHNISEVLLSQVALSVGFDTLACLYSHPCHVTGMGEKIVALPESKLV
YPILLVCPNFPVSSAEVYKNFRERRSIFSAESNLAGDHQAFEDMLSLPNDLTDAAIEIAPVISDVLKSIKNCKGNKVTKL
CGSGPTCIGVFDTVENLRDAHSELIRHHKWWVKIAHISL
>Mature_279_residues
MLTLRAPAKINLFLLITGRNESGFHLLDSVFCFTHDLYDEIIIVPTSATENSVKFIGASQAIGADNSVSRVLRFVNDYAK
RYFNVTLVKNLPVAAGIGGGSADAAALLRYFGGLHNISEVLLSQVALSVGFDTLACLYSHPCHVTGMGEKIVALPESKLV
YPILLVCPNFPVSSAEVYKNFRERRSIFSAESNLAGDHQAFEDMLSLPNDLTDAAIEIAPVISDVLKSIKNCKGNKVTKL
CGSGPTCIGVFDTVENLRDAHSELIRHHKWWVKIAHISL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_NEOSM (Q2GD50)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506596.1
- ProteinModelPortal:   Q2GD50
- STRING:   Q2GD50
- GeneID:   3931979
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0720
- TIGR:   NSE_0720
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   ELAMIMQ
- ProtClustDB:   CLSK2527715
- BioCyc:   NSEN222891:NSE_0720-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30417; Mature: 30417

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: ACT_SITE 9-9 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLRAPAKINLFLLITGRNESGFHLLDSVFCFTHDLYDEIIIVPTSATENSVKFIGASQ
CEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCC
AIGADNSVSRVLRFVNDYAKRYFNVTLVKNLPVAAGIGGGSADAAALLRYFGGLHNISEV
CCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCEEECCCCCCHHHHHHHHHHCCHHHHHHH
LLSQVALSVGFDTLACLYSHPCHVTGMGEKIVALPESKLVYPILLVCPNFPVSSAEVYKN
HHHHHHHHHCHHHHHHHHCCCCEEECCCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHH
FRERRSIFSAESNLAGDHQAFEDMLSLPNDLTDAAIEIAPVISDVLKSIKNCKGNKVTKL
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
CGSGPTCIGVFDTVENLRDAHSELIRHHKWWVKIAHISL
HCCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
>Mature Secondary Structure
MLTLRAPAKINLFLLITGRNESGFHLLDSVFCFTHDLYDEIIIVPTSATENSVKFIGASQ
CEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCC
AIGADNSVSRVLRFVNDYAKRYFNVTLVKNLPVAAGIGGGSADAAALLRYFGGLHNISEV
CCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCEEECCCCCCHHHHHHHHHHCCHHHHHHH
LLSQVALSVGFDTLACLYSHPCHVTGMGEKIVALPESKLVYPILLVCPNFPVSSAEVYKN
HHHHHHHHHCHHHHHHHHCCCCEEECCCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHH
FRERRSIFSAESNLAGDHQAFEDMLSLPNDLTDAAIEIAPVISDVLKSIKNCKGNKVTKL
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
CGSGPTCIGVFDTVENLRDAHSELIRHHKWWVKIAHISL
HCCCCCEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA