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The map label for this gene is maeB [C]

Identifier: 88608083

GI number: 88608083

Start: 342545

End: 344797

Strand: Direct

Name: maeB [C]

Synonym: NSE_0420

Alternate gene names: 88608083

Gene position: 342545-344797 (Clockwise)

Preceding gene: 88608677

Following gene: 88608493

Centisome position: 39.88

GC content: 43.32

Gene sequence:

>2253_bases
ATGGAAGGGGAAAGGGACGATCTTGGTGTACAAGCGTTACGGTATCACTCTAAGGGTAGAGCTGGTAAATTGGGGGTTCA
TGCCACAAAGCCTCTTCTTACGCAGGAAGATTTATCACTTGCCTACTCGCCTGGTGTAGCGCATCCGTGTTTGGAGATAG
AGAAAAATCCTCTAGATGTGTATAAGTACACCTCTAAAGGTAATTTGGTTGCAGTTATTTCAAATGGTACAGCTGTTCTT
GGCTTGGGAAACCTTGGAGCAATGGCCTCTAAACCTGTGATGGAAGGGAAGGCTGTGCTATTCAAAAAGTTTGCTGATAT
AGACTCAATTGATATTGAGGTCGATGCTCAGAGTATAGAGGAGTTTATCCTCACAGTGAAAAACATAGCTTCTACTTTTG
GTGGGATCAATCTTGAAGATATTAAGTCACCGGAGTGTTTTGAAATAGAGGAGCGTCTAGCAGCAATGCTAGATATTCCG
GTCTTTCACGATGATCAGCACGGCACAGCAATTATCGTATGTGCGGGTCTTATAAATGCTGCTGACATTACTATGAGAAA
GGTGGAGGAACTTAAGGTAGTCGTGAACGGCTGTGGTGCGGCAGGCATAGCATGCATCGAGTTGATGAAGGAGATCGGGG
TCAGTGATATAGTGGTCTGTGATCAAAGTGGTGTTATTAAAAAAGACAGAGATTTTCATGAAGGTGACCGAAAAGCTCTT
TATGCAGTTGATGTAACCGCGAACACGCTTGAGGAAGCACTGGTCGACGCTGATGTCTTTATTGGCTTATCCGCCGCAGA
TGTTCTGAAGCCGGAGTGGTTAAGTGGGATGAAGAAGGATCCGATAATTTTTGCTTTAGCTAATCCGGATCCTGAGATAA
AGCCAGAGTTAGCAAAGAAGGCGCGTCCGGATGCAATAATAGCAACTGGCAGATCGGATTATGATAATCAAATTAATAAC
GTTATGTGTTTTCCATACCTATTTAGGGGAGCACTTGATGTTGGTGCAAGTAAATTCAATACTGAAATGAAGCTTGCCGC
TGTGCATGCAATTGCTGCAATAGCGAAAAGGCCAATCTCAATGGAAGTGATGGATGCCTATGGGGATTCTCATATGCAAT
ATAGTAGGGAGTATATACTCCCAAAGCCTTTTGACCAGCGTCTTATTGCTGAAATCGCTCCGGCAGTTGCGCGTGCAGCA
GTTGAGACAGGTGTTGCAAAGAAGCCGATTTATAATTTTGATGCGTACAAGGATGATTTAGTCAGACGGTTGGCTTCGGG
TGCAACGACCCTATTCGGGATGGTGAGTAGTGTGCTCAGGGGTAATGGAAAGCGCATTATTTTTGCAGAAGGTGAAGAGC
TTAAGAGTATTCGAGCTGCGCTTCAGTGGCGTGACATAGGGTATGGTACTCCCATTCTTGTTGGAAGAAACAGTGTTATA
GAGGCCAAAATGGATGAAATGAATCTTCGCAATCGTGAAGGTTTTGAAGTAGCAAATGCGGCTGTTTCACAGAGAAGTGG
TGAGTACATAGAGTACATGTATAGCACCTTGCAAAGAAAAGGATTTCCTTATGAAAGATGTGTCAGAGATGTTGAAACGG
ATAATGACGTGTTTGCAGCTTGCATGCTTGCACTGAACGATGGAGATGCTGTGATCACTGGCTTAACGTGGAGTAACACA
GAGGATCGTCTCAAGAGTATTATGCCAATCGTTGGTGCTGCAGGTGTTGTTTTTGAAATGTCAATTATTGTTGCTGGTAT
AGGTACTTTTTTTATTTGCAATACTGGTATTAATGATACGCTAGATGCTGGGGATATTGCTGAAGTGGCAATAAAAGCTG
CTGAAGTAGCCAAAAGTATGGGATGCGTTCCAAGAGTTGCTGTGATAGCAAATTCGAATTTTGGAAGTGTAAATAGTCCT
AGTGCACTGATGGCACGTGAAGCAATTGAGATACTAGATGATTTTGGCGCTCCGTTTGAATACGAGGGCGAGATGGATCT
TGGTATGGCTTTAAACGAAAAATCTAAAAAGCTGTATCCTTTTTCTAGGCTTACGGATCGTGCGAATGTGCTCATTGTTC
AAAACGTGGAAGTTGTTGATGCAGTGTGTAAGTTTTCCAGTGAGATTATGGAAACAACTGTTATTGGACCTCTGTTGTTT
GGATTAAAGAGATCCGTGCAGATAGTGCGGCCGTCGTACGGTGCTACGGATATTCTAAATCTTGCTCTAATTGCTGCAAA
GACCTCTCTATGA

Upstream 100 bases:

>100_bases
TGGTGAAAGACATATTCTACTTAGGTATACAGAATGCTTTCTTTATTCCAAGGAAATGTCTTGTATGCTATAAGTTGGTG
TGATCTATGTGCATTGTGTT

Downstream 100 bases:

>100_bases
GTCAATTTTCATTTGCGGACTTATGGACTAACTGCACGTGTGTTACCTTCTCATCGCTTTTGGTTGTTTTTATGCTTTTT
CTATCATGAGTCCGATCTTG

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MEGERDDLGVQALRYHSKGRAGKLGVHATKPLLTQEDLSLAYSPGVAHPCLEIEKNPLDVYKYTSKGNLVAVISNGTAVL
GLGNLGAMASKPVMEGKAVLFKKFADIDSIDIEVDAQSIEEFILTVKNIASTFGGINLEDIKSPECFEIEERLAAMLDIP
VFHDDQHGTAIIVCAGLINAADITMRKVEELKVVVNGCGAAGIACIELMKEIGVSDIVVCDQSGVIKKDRDFHEGDRKAL
YAVDVTANTLEEALVDADVFIGLSAADVLKPEWLSGMKKDPIIFALANPDPEIKPELAKKARPDAIIATGRSDYDNQINN
VMCFPYLFRGALDVGASKFNTEMKLAAVHAIAAIAKRPISMEVMDAYGDSHMQYSREYILPKPFDQRLIAEIAPAVARAA
VETGVAKKPIYNFDAYKDDLVRRLASGATTLFGMVSSVLRGNGKRIIFAEGEELKSIRAALQWRDIGYGTPILVGRNSVI
EAKMDEMNLRNREGFEVANAAVSQRSGEYIEYMYSTLQRKGFPYERCVRDVETDNDVFAACMLALNDGDAVITGLTWSNT
EDRLKSIMPIVGAAGVVFEMSIIVAGIGTFFICNTGINDTLDAGDIAEVAIKAAEVAKSMGCVPRVAVIANSNFGSVNSP
SALMAREAIEILDDFGAPFEYEGEMDLGMALNEKSKKLYPFSRLTDRANVLIVQNVEVVDAVCKFSSEIMETTVIGPLLF
GLKRSVQIVRPSYGATDILNLALIAAKTSL

Sequences:

>Translated_750_residues
MEGERDDLGVQALRYHSKGRAGKLGVHATKPLLTQEDLSLAYSPGVAHPCLEIEKNPLDVYKYTSKGNLVAVISNGTAVL
GLGNLGAMASKPVMEGKAVLFKKFADIDSIDIEVDAQSIEEFILTVKNIASTFGGINLEDIKSPECFEIEERLAAMLDIP
VFHDDQHGTAIIVCAGLINAADITMRKVEELKVVVNGCGAAGIACIELMKEIGVSDIVVCDQSGVIKKDRDFHEGDRKAL
YAVDVTANTLEEALVDADVFIGLSAADVLKPEWLSGMKKDPIIFALANPDPEIKPELAKKARPDAIIATGRSDYDNQINN
VMCFPYLFRGALDVGASKFNTEMKLAAVHAIAAIAKRPISMEVMDAYGDSHMQYSREYILPKPFDQRLIAEIAPAVARAA
VETGVAKKPIYNFDAYKDDLVRRLASGATTLFGMVSSVLRGNGKRIIFAEGEELKSIRAALQWRDIGYGTPILVGRNSVI
EAKMDEMNLRNREGFEVANAAVSQRSGEYIEYMYSTLQRKGFPYERCVRDVETDNDVFAACMLALNDGDAVITGLTWSNT
EDRLKSIMPIVGAAGVVFEMSIIVAGIGTFFICNTGINDTLDAGDIAEVAIKAAEVAKSMGCVPRVAVIANSNFGSVNSP
SALMAREAIEILDDFGAPFEYEGEMDLGMALNEKSKKLYPFSRLTDRANVLIVQNVEVVDAVCKFSSEIMETTVIGPLLF
GLKRSVQIVRPSYGATDILNLALIAAKTSL
>Mature_750_residues
MEGERDDLGVQALRYHSKGRAGKLGVHATKPLLTQEDLSLAYSPGVAHPCLEIEKNPLDVYKYTSKGNLVAVISNGTAVL
GLGNLGAMASKPVMEGKAVLFKKFADIDSIDIEVDAQSIEEFILTVKNIASTFGGINLEDIKSPECFEIEERLAAMLDIP
VFHDDQHGTAIIVCAGLINAADITMRKVEELKVVVNGCGAAGIACIELMKEIGVSDIVVCDQSGVIKKDRDFHEGDRKAL
YAVDVTANTLEEALVDADVFIGLSAADVLKPEWLSGMKKDPIIFALANPDPEIKPELAKKARPDAIIATGRSDYDNQINN
VMCFPYLFRGALDVGASKFNTEMKLAAVHAIAAIAKRPISMEVMDAYGDSHMQYSREYILPKPFDQRLIAEIAPAVARAA
VETGVAKKPIYNFDAYKDDLVRRLASGATTLFGMVSSVLRGNGKRIIFAEGEELKSIRAALQWRDIGYGTPILVGRNSVI
EAKMDEMNLRNREGFEVANAAVSQRSGEYIEYMYSTLQRKGFPYERCVRDVETDNDVFAACMLALNDGDAVITGLTWSNT
EDRLKSIMPIVGAAGVVFEMSIIVAGIGTFFICNTGINDTLDAGDIAEVAIKAAEVAKSMGCVPRVAVIANSNFGSVNSP
SALMAREAIEILDDFGAPFEYEGEMDLGMALNEKSKKLYPFSRLTDRANVLIVQNVEVVDAVCKFSSEIMETTVIGPLLF
GLKRSVQIVRPSYGATDILNLALIAAKTSL

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788806, Length=760, Percent_Identity=45.3947368421053, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1788635, Length=313, Percent_Identity=24.9201277955272, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788800, Length=316, Percent_Identity=24.6835443037975, Blast_Score=73, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17537199, Length=400, Percent_Identity=25, Blast_Score=72, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=271, Percent_Identity=25.4612546125461, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 81276; Mature: 81276

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGERDDLGVQALRYHSKGRAGKLGVHATKPLLTQEDLSLAYSPGVAHPCLEIEKNPLDV
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHCCCCCCEE
YKYTSKGNLVAVISNGTAVLGLGNLGAMASKPVMEGKAVLFKKFADIDSIDIEVDAQSIE
EEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECHHHHH
EFILTVKNIASTFGGINLEDIKSPECFEIEERLAAMLDIPVFHDDQHGTAIIVCAGLINA
HHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEEEEEHHHCH
ADITMRKVEELKVVVNGCGAAGIACIELMKEIGVSDIVVCDQSGVIKKDRDFHEGDRKAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEE
YAVDVTANTLEEALVDADVFIGLSAADVLKPEWLSGMKKDPIIFALANPDPEIKPELAKK
EEEECCHHHHHHHHHCCHHEEECCHHHHCCCHHHCCCCCCCEEEEECCCCCCCCHHHHHC
ARPDAIIATGRSDYDNQINNVMCFPYLFRGALDVGASKFNTEMKLAAVHAIAAIAKRPIS
CCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHCCCC
MEVMDAYGDSHMQYSREYILPKPFDQRLIAEIAPAVARAAVETGVAKKPIYNFDAYKDDL
EEHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
VRRLASGATTLFGMVSSVLRGNGKRIIFAEGEELKSIRAALQWRDIGYGTPILVGRNSVI
HHHHHHCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCE
EAKMDEMNLRNREGFEVANAAVSQRSGEYIEYMYSTLQRKGFPYERCVRDVETDNDVFAA
EHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHEE
CMLALNDGDAVITGLTWSNTEDRLKSIMPIVGAAGVVFEMSIIVAGIGTFFICNTGINDT
HHEEECCCCEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCCCC
LDAGDIAEVAIKAAEVAKSMGCVPRVAVIANSNFGSVNSPSALMAREAIEILDDFGAPFE
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEE
YEGEMDLGMALNEKSKKLYPFSRLTDRANVLIVQNVEVVDAVCKFSSEIMETTVIGPLLF
ECCCCCCCEEECCCCCCCCCHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
GLKRSVQIVRPSYGATDILNLALIAAKTSL
HHHCCEEEECCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEGERDDLGVQALRYHSKGRAGKLGVHATKPLLTQEDLSLAYSPGVAHPCLEIEKNPLDV
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHCCCCCCEE
YKYTSKGNLVAVISNGTAVLGLGNLGAMASKPVMEGKAVLFKKFADIDSIDIEVDAQSIE
EEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECHHHHH
EFILTVKNIASTFGGINLEDIKSPECFEIEERLAAMLDIPVFHDDQHGTAIIVCAGLINA
HHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEEEEEHHHCH
ADITMRKVEELKVVVNGCGAAGIACIELMKEIGVSDIVVCDQSGVIKKDRDFHEGDRKAL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEE
YAVDVTANTLEEALVDADVFIGLSAADVLKPEWLSGMKKDPIIFALANPDPEIKPELAKK
EEEECCHHHHHHHHHCCHHEEECCHHHHCCCHHHCCCCCCCEEEEECCCCCCCCHHHHHC
ARPDAIIATGRSDYDNQINNVMCFPYLFRGALDVGASKFNTEMKLAAVHAIAAIAKRPIS
CCCCEEEEECCCCCCHHHCCEEEHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHCCCC
MEVMDAYGDSHMQYSREYILPKPFDQRLIAEIAPAVARAAVETGVAKKPIYNFDAYKDDL
EEHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
VRRLASGATTLFGMVSSVLRGNGKRIIFAEGEELKSIRAALQWRDIGYGTPILVGRNSVI
HHHHHHCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCE
EAKMDEMNLRNREGFEVANAAVSQRSGEYIEYMYSTLQRKGFPYERCVRDVETDNDVFAA
EHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHEE
CMLALNDGDAVITGLTWSNTEDRLKSIMPIVGAAGVVFEMSIIVAGIGTFFICNTGINDT
HHEEECCCCEEEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCCCCC
LDAGDIAEVAIKAAEVAKSMGCVPRVAVIANSNFGSVNSPSALMAREAIEILDDFGAPFE
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEE
YEGEMDLGMALNEKSKKLYPFSRLTDRANVLIVQNVEVVDAVCKFSSEIMETTVIGPLLF
ECCCCCCCEEECCCCCCCCCHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHH
GLKRSVQIVRPSYGATDILNLALIAAKTSL
HHHCCEEEECCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]