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The map label for this gene is rne [H]

Identifier: 88608043

GI number: 88608043

Start: 97557

End: 99206

Strand: Direct

Name: rne [H]

Synonym: NSE_0120

Alternate gene names: 88608043

Gene position: 97557-99206 (Clockwise)

Preceding gene: 88608453

Following gene: 88608448

Centisome position: 11.36

GC content: 40.85

Gene sequence:

>1650_bases
TTGAAGAAAATACTAATAGACTCAAGAGACGGTGAAACCAGGCTTGCTATTCTCAGGAGTGGTCTTCTTGTTGGGTATGA
TTGTGAGCATACTAACAGTTTGCCCCAAAAGGGAAATATATACCAGGGAATAGTTTTTAAGGTTGATGATTCTCTCCAAG
CCGCTTTTGTTGAGTATTTACCCGGTGAATATGGATTCTTGCCATTTTCTGAAATTCCAGTAATCTACTTTGACCTGCTT
GAGGAGGTCAAGGCAAATGCGATAGCTGAGGGCAAACATGTCAACCTGTATAAGCATTTCAACGCAACCGAGGTGATCAA
AAAGGGACTTGTTCTTTTCGTACAAGTTACTAAGGAGAAGAGAAGGACCAAGGATGTTACTTTGACGGCATATGTAAAAC
TGCCAGGTAGATATTGTGTCCTAATGCCATTTTTGAAGGATTTAATCTTGTCCAAGCAAATTGTGGATGAGCAAGCCGTC
AGTAGGCTGACGCATTTTGCTGAGGAAGAGATGGCAAATAGAAAGTGCGGCGTTGTATTCAAGCCGTATTGTGCAGATGC
TGATAAAAAAAATATTTTAGGAGACTACTGTTCGTTGCTCTCTGAGTGGGAGAAAGTAGTTTCGCAGAATGATAAAAGCA
AGGCGCCAAGACTAGTTTTCCGCGAAGCCAATCTTGTGAAAAAATTCTTGAGAGATTCATATGACGGAAGTGTAGGGGAG
GTAATTGTTAATCATAGTGATGTCTATCAGGAAGCAAAGGACTACATACAAAATTCTCTCCACTTTGAAAATCCCAATAT
CACACTCTATTCGGATACTTTACCTGTTTTTGATAAGTACAAAGTTGAACCTCAAGTTGATGGTTTGTACGAGGAGACAA
TTTATCTGAGGAACGGTGCTTATATCGTCATTAACACTACCGAAGCTCTCACAGCAGTGGATGTAAATTCTGGTAGGATG
AAAAAAGAAAGCAATATCGAGGAGACTGCTTATAAGGTGAATATGGAGGCAGTCGCTGAGATTGTCCGTCATCTCGAACT
TAGGGGAATTGCTGGTATTGTCGTGATAGATTTCATTGACATGAATAATTCCAAATATATGCTGAATGTTGAAAGGGCGC
TGTTGGATGCTTTCAAAAGATATCGGTCAAGGGTGCAGATTGCCCCGATTAATAAGTTTGGACTCATTGCGCTTTCTAAA
CAAAGGACCAAACATAACATTCTTGACTCTCATACTGTGAAATGTCCTTGTTGTGAGGGCAAGGGTAAAGTACTAAAGAT
GGAACATCAGGTTCGTAGGGTTTTTTCAGCCATATATACAGCACCTCGCTGCAGGGAGGTTACTGTGACTGCCAATCCTG
AATTAGCAGTAGCTGTATTTAATGGCTATCGCAAGATGCTAGCTCAAGCAGAAAAAGTTGTTGGAGGGAGCATAGTTTTC
AAGATTGATGAAAAACTTGGGCTTGCGAAGGGTTTTGAGATTGCCTTTCACGGAAAGGATGATGTAGAGATATTACGAGA
GGACCAAGTTTTAACGACTGTAGAAGATCAGTCGAATGAAAAGACGGTCACAGAGGGAAGCTCTGTAGTAAAGGTGGAAT
CTAGAAGATCGTGGCTTGCATCTTGGATAAAAAAACTCACAGGACTATAA

Upstream 100 bases:

>100_bases
CCATTTCAATGTTTCACTATAAGCTCTTTGCTCATTGATTGCTGAAACTACATAGGTTGGGTAGAATGGAGGAATTGTCT
AGCGTTTTAAAGGGAATTAC

Downstream 100 bases:

>100_bases
AGAGTTTCTGGACTTAAGAGTCTCATTTCCTTTTTCATTCTTTAGAAGAGTCGATGTAAGGAGAATTACGAACAGTCCAC
CGGATAAGATACTCAAGCTG

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MKKILIDSRDGETRLAILRSGLLVGYDCEHTNSLPQKGNIYQGIVFKVDDSLQAAFVEYLPGEYGFLPFSEIPVIYFDLL
EEVKANAIAEGKHVNLYKHFNATEVIKKGLVLFVQVTKEKRRTKDVTLTAYVKLPGRYCVLMPFLKDLILSKQIVDEQAV
SRLTHFAEEEMANRKCGVVFKPYCADADKKNILGDYCSLLSEWEKVVSQNDKSKAPRLVFREANLVKKFLRDSYDGSVGE
VIVNHSDVYQEAKDYIQNSLHFENPNITLYSDTLPVFDKYKVEPQVDGLYEETIYLRNGAYIVINTTEALTAVDVNSGRM
KKESNIEETAYKVNMEAVAEIVRHLELRGIAGIVVIDFIDMNNSKYMLNVERALLDAFKRYRSRVQIAPINKFGLIALSK
QRTKHNILDSHTVKCPCCEGKGKVLKMEHQVRRVFSAIYTAPRCREVTVTANPELAVAVFNGYRKMLAQAEKVVGGSIVF
KIDEKLGLAKGFEIAFHGKDDVEILREDQVLTTVEDQSNEKTVTEGSSVVKVESRRSWLASWIKKLTGL

Sequences:

>Translated_549_residues
MKKILIDSRDGETRLAILRSGLLVGYDCEHTNSLPQKGNIYQGIVFKVDDSLQAAFVEYLPGEYGFLPFSEIPVIYFDLL
EEVKANAIAEGKHVNLYKHFNATEVIKKGLVLFVQVTKEKRRTKDVTLTAYVKLPGRYCVLMPFLKDLILSKQIVDEQAV
SRLTHFAEEEMANRKCGVVFKPYCADADKKNILGDYCSLLSEWEKVVSQNDKSKAPRLVFREANLVKKFLRDSYDGSVGE
VIVNHSDVYQEAKDYIQNSLHFENPNITLYSDTLPVFDKYKVEPQVDGLYEETIYLRNGAYIVINTTEALTAVDVNSGRM
KKESNIEETAYKVNMEAVAEIVRHLELRGIAGIVVIDFIDMNNSKYMLNVERALLDAFKRYRSRVQIAPINKFGLIALSK
QRTKHNILDSHTVKCPCCEGKGKVLKMEHQVRRVFSAIYTAPRCREVTVTANPELAVAVFNGYRKMLAQAEKVVGGSIVF
KIDEKLGLAKGFEIAFHGKDDVEILREDQVLTTVEDQSNEKTVTEGSSVVKVESRRSWLASWIKKLTGL
>Mature_549_residues
MKKILIDSRDGETRLAILRSGLLVGYDCEHTNSLPQKGNIYQGIVFKVDDSLQAAFVEYLPGEYGFLPFSEIPVIYFDLL
EEVKANAIAEGKHVNLYKHFNATEVIKKGLVLFVQVTKEKRRTKDVTLTAYVKLPGRYCVLMPFLKDLILSKQIVDEQAV
SRLTHFAEEEMANRKCGVVFKPYCADADKKNILGDYCSLLSEWEKVVSQNDKSKAPRLVFREANLVKKFLRDSYDGSVGE
VIVNHSDVYQEAKDYIQNSLHFENPNITLYSDTLPVFDKYKVEPQVDGLYEETIYLRNGAYIVINTTEALTAVDVNSGRM
KKESNIEETAYKVNMEAVAEIVRHLELRGIAGIVVIDFIDMNNSKYMLNVERALLDAFKRYRSRVQIAPINKFGLIALSK
QRTKHNILDSHTVKCPCCEGKGKVLKMEHQVRRVFSAIYTAPRCREVTVTANPELAVAVFNGYRKMLAQAEKVVGGSIVF
KIDEKLGLAKGFEIAFHGKDDVEILREDQVLTTVEDQSNEKTVTEGSSVVKVESRRSWLASWIKKLTGL

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=428, Percent_Identity=32.9439252336449, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI87082235, Length=484, Percent_Identity=27.4793388429752, Blast_Score=181, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 62197; Mature: 62197

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILIDSRDGETRLAILRSGLLVGYDCEHTNSLPQKGNIYQGIVFKVDDSLQAAFVEYL
CCEEEEECCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHC
PGEYGFLPFSEIPVIYFDLLEEVKANAIAEGKHVNLYKHFNATEVIKKGLVLFVQVTKEK
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCEEEEEEEHHHH
RRTKDVTLTAYVKLPGRYCVLMPFLKDLILSKQIVDEQAVSRLTHFAEEEMANRKCGVVF
HCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
KPYCADADKKNILGDYCSLLSEWEKVVSQNDKSKAPRLVFREANLVKKFLRDSYDGSVGE
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHE
VIVNHSDVYQEAKDYIQNSLHFENPNITLYSDTLPVFDKYKVEPQVDGLYEETIYLRNGA
EEECCHHHHHHHHHHHHHCCEECCCCEEEEECCCCCCCCEECCCCCCCCEEEEEEEECCC
YIVINTTEALTAVDVNSGRMKKESNIEETAYKVNMEAVAEIVRHLELRGIAGIVVIDFID
EEEEECCCEEEEEECCCCCCCCCCCCHHHHHEECHHHHHHHHHHHHHCCCCEEEEEEEEE
MNNSKYMLNVERALLDAFKRYRSRVQIAPINKFGLIALSKQRTKHNILDSHTVKCPCCEG
CCCCEEEEEHHHHHHHHHHHHHCCEEEEECCCCEEEEEECHHHHHHCCCCCCEECCCCCC
KGKVLKMEHQVRRVFSAIYTAPRCREVTVTANPELAVAVFNGYRKMLAQAEKVVGGSIVF
CCCEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCEEEE
KIDEKLGLAKGFEIAFHGKDDVEILREDQVLTTVEDQSNEKTVTEGSSVVKVESRRSWLA
EECCCCCCCCCCEEEECCCCCHHHHCCCCEEEEECCCCCCCEECCCCCEEEEHHHHHHHH
SWIKKLTGL
HHHHHHHCC
>Mature Secondary Structure
MKKILIDSRDGETRLAILRSGLLVGYDCEHTNSLPQKGNIYQGIVFKVDDSLQAAFVEYL
CCEEEEECCCCCHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEEEEECCHHHHHHHHHC
PGEYGFLPFSEIPVIYFDLLEEVKANAIAEGKHVNLYKHFNATEVIKKGLVLFVQVTKEK
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCEEEEEEEHHHH
RRTKDVTLTAYVKLPGRYCVLMPFLKDLILSKQIVDEQAVSRLTHFAEEEMANRKCGVVF
HCCCCEEEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
KPYCADADKKNILGDYCSLLSEWEKVVSQNDKSKAPRLVFREANLVKKFLRDSYDGSVGE
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHE
VIVNHSDVYQEAKDYIQNSLHFENPNITLYSDTLPVFDKYKVEPQVDGLYEETIYLRNGA
EEECCHHHHHHHHHHHHHCCEECCCCEEEEECCCCCCCCEECCCCCCCCEEEEEEEECCC
YIVINTTEALTAVDVNSGRMKKESNIEETAYKVNMEAVAEIVRHLELRGIAGIVVIDFID
EEEEECCCEEEEEECCCCCCCCCCCCHHHHHEECHHHHHHHHHHHHHCCCCEEEEEEEEE
MNNSKYMLNVERALLDAFKRYRSRVQIAPINKFGLIALSKQRTKHNILDSHTVKCPCCEG
CCCCEEEEEHHHHHHHHHHHHHCCEEEEECCCCEEEEEECHHHHHHCCCCCCEECCCCCC
KGKVLKMEHQVRRVFSAIYTAPRCREVTVTANPELAVAVFNGYRKMLAQAEKVVGGSIVF
CCCEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCEEEE
KIDEKLGLAKGFEIAFHGKDDVEILREDQVLTTVEDQSNEKTVTEGSSVVKVESRRSWLA
EECCCCCCCCCCEEEECCCCCHHHHCCCCEEEEECCCCCCCEECCCCCEEEEHHHHHHHH
SWIKKLTGL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]