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The map label for this gene is pdhA

Identifier: 88608016

GI number: 88608016

Start: 712430

End: 713434

Strand: Reverse

Name: pdhA

Synonym: NSE_0802

Alternate gene names: 88608016

Gene position: 713434-712430 (Counterclockwise)

Preceding gene: 88608094

Following gene: 88608071

Centisome position: 83.05

GC content: 47.26

Gene sequence:

>1005_bases
TTGCCTGGTTGCGTATTTGATTGGTACCTCAGATGTATGTTGCTTGAACCTTTAGATTCGTCGGTTCTTTTGCCTCTTTA
TGAGAAAATGCTGTTGATCCGGCGCTTCGAAGAGAGGGCGGGGCAGCTCTACAGCATGGGCGAGATATGCGGTTTTTGTC
ATCTTTACATAGGACAGGAAGCGGTTGCTGTTGGTCTGGACTACTGTTTGAAGAGGGAGGATTCTGTCATTACTAGCTAC
AGGGATCATGGTATGATGCTTGTCCGAGGTTCTTCTCCCGACGTGATGATGGCTGAACTTTTAGGGAAATCCTCCGGTTG
TTCTAATGGTAAGGGTGGGTCTATGCATATGTTTGATCCTGAGAGGAATTTCTTCGGGGGTCATGGTATTGTTGGTTCGC
AAGTCTCCTTAGGGACAGGTATAGCTTTTGCTGAGAAATACAGAGACTCTAACGCTGTTGTTGCGAGTTGTTTTGGGGAT
GGCGCGATTAACCAGGGGCAGGTCTATGAATCTTTTAATATGGCTGCCCTGTGGAAACTTCCTATACTTTATGTTGTGGA
AAATAACATGTACGCTATGGGTAGCTCCGTTGAGAGCGTGTGCGCGAATTCTTCGCTTTCCAATAGGGGTGAATCGTTTG
GAATACCTGGCTACTCTGCTAATGGTATGGATCTGATGGATGTGATTCGTGTCACGATGAATGCAGTTGAGGGTGTGCGG
GGCGGAAGCGGTCCGGTTCTTGTAGAGTATAAAACTTATCGATTCAAGGGGCACTCCATGTCTGATCCAGCTTCTTATAG
GTCTAAGGAGGAGGTTTCTTCCTTCAAGGAGCGGGATCCTCTGAAAAGTGTGGAAACCTTGCTTCTGCAGGCAGGCGTGT
TGCACAGCGAGCTAGAACGTGTGCAAAAGGCAGTTAAAGATCAGGTATCGAGTAGTGTGGCTTATGCACGCGCCTCTACT
TTTCCTGATAAGGCAAGTCTCATGACGGATGTTTATTCAGAGTGA

Upstream 100 bases:

>100_bases
TCGACTCCATTCCGAACTCGAAAGTGAAACCTTTTTGCGCCGATGGTACTGCTTGTGTGGGAGAGTAGGTGGTTGCTAGG
TCCTTGAAATTACAGACGTT

Downstream 100 bases:

>100_bases
AAGTAACTTTCTCATGGAAAGTTGTGGGTTTTCGTGTGGCTTGCAATTTTTCTTCGTGGTGTTAGCATTTCGGTGTTTCC
GTGTGGAAACTGAGGATGGT

Product: pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MPGCVFDWYLRCMLLEPLDSSVLLPLYEKMLLIRRFEERAGQLYSMGEICGFCHLYIGQEAVAVGLDYCLKREDSVITSY
RDHGMMLVRGSSPDVMMAELLGKSSGCSNGKGGSMHMFDPERNFFGGHGIVGSQVSLGTGIAFAEKYRDSNAVVASCFGD
GAINQGQVYESFNMAALWKLPILYVVENNMYAMGSSVESVCANSSLSNRGESFGIPGYSANGMDLMDVIRVTMNAVEGVR
GGSGPVLVEYKTYRFKGHSMSDPASYRSKEEVSSFKERDPLKSVETLLLQAGVLHSELERVQKAVKDQVSSSVAYARAST
FPDKASLMTDVYSE

Sequences:

>Translated_334_residues
MPGCVFDWYLRCMLLEPLDSSVLLPLYEKMLLIRRFEERAGQLYSMGEICGFCHLYIGQEAVAVGLDYCLKREDSVITSY
RDHGMMLVRGSSPDVMMAELLGKSSGCSNGKGGSMHMFDPERNFFGGHGIVGSQVSLGTGIAFAEKYRDSNAVVASCFGD
GAINQGQVYESFNMAALWKLPILYVVENNMYAMGSSVESVCANSSLSNRGESFGIPGYSANGMDLMDVIRVTMNAVEGVR
GGSGPVLVEYKTYRFKGHSMSDPASYRSKEEVSSFKERDPLKSVETLLLQAGVLHSELERVQKAVKDQVSSSVAYARAST
FPDKASLMTDVYSE
>Mature_333_residues
PGCVFDWYLRCMLLEPLDSSVLLPLYEKMLLIRRFEERAGQLYSMGEICGFCHLYIGQEAVAVGLDYCLKREDSVITSYR
DHGMMLVRGSSPDVMMAELLGKSSGCSNGKGGSMHMFDPERNFFGGHGIVGSQVSLGTGIAFAEKYRDSNAVVASCFGDG
AINQGQVYESFNMAALWKLPILYVVENNMYAMGSSVESVCANSSLSNRGESFGIPGYSANGMDLMDVIRVTMNAVEGVRG
GSGPVLVEYKTYRFKGHSMSDPASYRSKEEVSSFKERDPLKSVETLLLQAGVLHSELERVQKAVKDQVSSSVAYARASTF
PDKASLMTDVYSE

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=313, Percent_Identity=44.0894568690096, Blast_Score=264, Evalue=8e-71,
Organism=Homo sapiens, GI291084742, Length=313, Percent_Identity=43.7699680511182, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI4505685, Length=313, Percent_Identity=43.7699680511182, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI291084744, Length=320, Percent_Identity=42.8125, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI291084757, Length=313, Percent_Identity=38.6581469648562, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI11386135, Length=320, Percent_Identity=29.0625, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI258645172, Length=320, Percent_Identity=29.0625, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564172, Length=318, Percent_Identity=42.1383647798742, Blast_Score=257, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI17536047, Length=318, Percent_Identity=42.1383647798742, Blast_Score=257, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI86563355, Length=321, Percent_Identity=28.9719626168224, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI86563357, Length=321, Percent_Identity=28.9719626168224, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321026, Length=343, Percent_Identity=41.6909620991254, Blast_Score=274, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24639744, Length=313, Percent_Identity=44.408945686901, Blast_Score=270, Evalue=9e-73,
Organism=Drosophila melanogaster, GI28571106, Length=313, Percent_Identity=44.408945686901, Blast_Score=270, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24639740, Length=313, Percent_Identity=44.408945686901, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24639746, Length=307, Percent_Identity=44.6254071661238, Blast_Score=265, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24639748, Length=314, Percent_Identity=45.859872611465, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI21355903, Length=334, Percent_Identity=24.8502994011976, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017597 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36584; Mature: 36453

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGCVFDWYLRCMLLEPLDSSVLLPLYEKMLLIRRFEERAGQLYSMGEICGFCHLYIGQE
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
AVAVGLDYCLKREDSVITSYRDHGMMLVRGSSPDVMMAELLGKSSGCSNGKGGSMHMFDP
HHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCEEEEECC
ERNFFGGHGIVGSQVSLGTGIAFAEKYRDSNAVVASCFGDGAINQGQVYESFNMAALWKL
CCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHCCCCEEHHHC
PILYVVENNMYAMGSSVESVCANSSLSNRGESFGIPGYSANGMDLMDVIRVTMNAVEGVR
CEEEEEECCEEECCCCHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GGSGPVLVEYKTYRFKGHSMSDPASYRSKEEVSSFKERDPLKSVETLLLQAGVLHSELER
CCCCCEEEEEEEEEECCCCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VQKAVKDQVSSSVAYARASTFPDKASLMTDVYSE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PGCVFDWYLRCMLLEPLDSSVLLPLYEKMLLIRRFEERAGQLYSMGEICGFCHLYIGQE
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
AVAVGLDYCLKREDSVITSYRDHGMMLVRGSSPDVMMAELLGKSSGCSNGKGGSMHMFDP
HHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCCCCCCEEEEECC
ERNFFGGHGIVGSQVSLGTGIAFAEKYRDSNAVVASCFGDGAINQGQVYESFNMAALWKL
CCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHCCCCEEHHHC
PILYVVENNMYAMGSSVESVCANSSLSNRGESFGIPGYSANGMDLMDVIRVTMNAVEGVR
CEEEEEECCEEECCCCHHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GGSGPVLVEYKTYRFKGHSMSDPASYRSKEEVSSFKERDPLKSVETLLLQAGVLHSELER
CCCCCEEEEEEEEEECCCCCCCCHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VQKAVKDQVSSSVAYARASTFPDKASLMTDVYSE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]