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The map label for this gene is nuoI

Identifier: 88608014

GI number: 88608014

Start: 729277

End: 729759

Strand: Direct

Name: nuoI

Synonym: NSE_0820

Alternate gene names: 88608014

Gene position: 729277-729759 (Clockwise)

Preceding gene: 88608251

Following gene: 88608844

Centisome position: 84.9

GC content: 42.03

Gene sequence:

>483_bases
ATGTTTAAGTTCCTAAGCCAGATGCAGCTGACCCGCTTCTTTGTTGGTTTCAGGACCGTCTTGTGGTACTTTTTTGCACC
AAAGGTTACGATAAAATATCCACAAGAGAAGGGACCCATAAGTCTGCGTTTCAGAGGTGAACACGCGCTAAGACGCTACA
AAAATGGGGAAGAGCGTTGCATAGCTTGTAAGTTATGTGAGGTGGTCTGTCCTGCCCAGGCAATCACGATTGAAGCAGCT
CCCAGAGAATCTGATGGAAGTAGGCGTGCCACAAAATACGATATAGATATGACTAAGTGCATTTATTGTGGCTTTTGTCA
GGAAGCTTGCCCTGTAGATGCAATTGTTGAAGGACCGAATTTTGAGTTTGCAAGGGAAAATCGTGAAGATTTGCTCTATG
ACAAAAAAAAATTACTCGATAATGGCAGTAAATGGGAAAGTGCATTAATTAAGATGCTCAATAGAAATGACACAACTCAT
TGA

Upstream 100 bases:

>100_bases
ACCACCCAAAACCGTTTCATGTACCAAGTGATGTTTTGTAGAACACACATTTTACTTAACAAACCACATATGTTAGCATT
TCGCTGTTCTTGGTCGGTGT

Downstream 100 bases:

>100_bases
TGGTTTCCGAATTGCCGCAGGTATCTGTGAATCTATTGCAACTTCTGTAGCACACCTCAGGAAAAACAATGGCTTAGTAC
CTAGTCTGAGAGTCATCATT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MFKFLSQMQLTRFFVGFRTVLWYFFAPKVTIKYPQEKGPISLRFRGEHALRRYKNGEERCIACKLCEVVCPAQAITIEAA
PRESDGSRRATKYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFARENREDLLYDKKKLLDNGSKWESALIKMLNRNDTTH

Sequences:

>Translated_160_residues
MFKFLSQMQLTRFFVGFRTVLWYFFAPKVTIKYPQEKGPISLRFRGEHALRRYKNGEERCIACKLCEVVCPAQAITIEAA
PRESDGSRRATKYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFARENREDLLYDKKKLLDNGSKWESALIKMLNRNDTTH
>Mature_160_residues
MFKFLSQMQLTRFFVGFRTVLWYFFAPKVTIKYPQEKGPISLRFRGEHALRRYKNGEERCIACKLCEVVCPAQAITIEAA
PRESDGSRRATKYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFARENREDLLYDKKKLLDNGSKWESALIKMLNRNDTTH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=139, Percent_Identity=72.6618705035971, Blast_Score=216, Evalue=7e-57,
Organism=Escherichia coli, GI1788617, Length=142, Percent_Identity=40.1408450704225, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI1789075, Length=113, Percent_Identity=30.0884955752212, Blast_Score=60, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=149, Percent_Identity=67.1140939597315, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI17864306, Length=151, Percent_Identity=67.5496688741722, Blast_Score=212, Evalue=8e-56,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_NEOSM (Q2GCV4)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506692.1
- ProteinModelPortal:   Q2GCV4
- SMR:   Q2GCV4
- STRING:   Q2GCV4
- GeneID:   3931951
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0820
- TIGR:   NSE_0820
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   HALKVSH
- PhylomeDB:   Q2GCV4
- ProtClustDB:   PRK05888
- BioCyc:   NSEN222891:NSE_0820-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 18625; Mature: 18625

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKFLSQMQLTRFFVGFRTVLWYFFAPKVTIKYPQEKGPISLRFRGEHALRRYKNGEERC
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCHHHHHHCCCCCHHH
IACKLCEVVCPAQAITIEAAPRESDGSRRATKYDIDMTKCIYCGFCQEACPVDAIVEGPN
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCEEEECCC
FEFARENREDLLYDKKKLLDNGSKWESALIKMLNRNDTTH
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFKFLSQMQLTRFFVGFRTVLWYFFAPKVTIKYPQEKGPISLRFRGEHALRRYKNGEERC
CCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCHHHHHHCCCCCHHH
IACKLCEVVCPAQAITIEAAPRESDGSRRATKYDIDMTKCIYCGFCQEACPVDAIVEGPN
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCEEEECCC
FEFARENREDLLYDKKKLLDNGSKWESALIKMLNRNDTTH
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA