Click here to switch to the map view.

The map label for this gene is recA

Identifier: 88608011

GI number: 88608011

Start: 456455

End: 457438

Strand: Reverse

Name: recA

Synonym: NSE_0530

Alternate gene names: 88608011

Gene position: 457438-456455 (Counterclockwise)

Preceding gene: 88608730

Following gene: 88608093

Centisome position: 53.25

GC content: 44.0

Gene sequence:

>984_bases
ATGAACAGGGAAGATGCGCTTAAGGGCGTTGTGGTTCAAATTGAAAAAATGTTTGGGAAAGGTTCCATAATGACCTTTAG
TCAGGAGCCAGCTTCAGTGGAGTGCATTTCTACGGGTTCCATAGGATTGGATAGTGCTCTTGGTGGAGGTATACCAAAGG
GGCGAATTATTGAGATATTTGGACCGGAAAGTTCTGGGAAGACGACACTTACGCTTCACATAATAGCTGAAGCGCAGAAA
AACGGCGGGACGTGTGCGTTTATTGACGCTGAGCATGCTTTGGATCCTGTTTATGCAAGAAGGTTGGGTGTCAAAATTGA
GGAGCTGTTAATTTCGCAGCCGGATACTGGGGAGCAGGCTTTGGAAATAGTAGATAGTCTTATTAAGTCTGGTGCAGTCG
ATGTGATAGTTGTCGATTCTGTTGCTGCTCTTACTCCGAAAGCAGAAATAGAAGGAGAAATGGGAGATCTACATGTTGGG
CTTCAGGCTAGATTGCTTAGTCAGGCACTGCGAAAGATCACAGCTTCTGTATCCCGTAGTAACTGCGTTGTGGTCTTCAT
CAACCAGATCCGGATGAAGATCGGTGTCATGTTTGGTAGTCCTGAAACAACGACAGGGGGTAATGCATTGAAATTCTATT
CGTCCGTTAGAATTGATATTCGCAAGATTGGTTCAATTAAGGATAAAGAAGATGTTGTGGGTAACCAGACACGGGTGAAG
GTGGTTAAAAATAAGGTAGCGCCTCCGTTTAAGCAGGCTGAGTTTGATATCATTTACACTGAGGGCATTTCGAAGCTTGG
TGAAATAATCGAGATTGGTGTGCGCAATAAGATAATAGAAAAGGCAGGTTCGTATTATTCATACGGGGATGTCAAGCTTG
GTCAGGGTAAGGAAAATGTGAAGCAGTTCTTAAAAGAGAATAAAGAGTTAGCAGCGGAGTTAGAGAGGAAAGTGAGGGAT
TCCATGCTGCTTATTGCAACGTAG

Upstream 100 bases:

>100_bases
AAGAGATTTGCATAGGTGATGCCAGTGTAGTAATGGCATTGTTCCTAAGTAAATTGTGTCATACAATGTCTGGGAACGTG
AGGGTTGCGAGTTGATTTTA

Downstream 100 bases:

>100_bases
CATGCAGTGTGATTTATATTTTAGTTTTTTTAGCGACCGTGAATAAGTACTATATCTATGAGGGTGATGTGCCCGCTGAC
TTCGCTCCATCTGATGCAAT

Product: recA protein

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNREDALKGVVVQIEKMFGKGSIMTFSQEPASVECISTGSIGLDSALGGGIPKGRIIEIFGPESSGKTTLTLHIIAEAQK
NGGTCAFIDAEHALDPVYARRLGVKIEELLISQPDTGEQALEIVDSLIKSGAVDVIVVDSVAALTPKAEIEGEMGDLHVG
LQARLLSQALRKITASVSRSNCVVVFINQIRMKIGVMFGSPETTTGGNALKFYSSVRIDIRKIGSIKDKEDVVGNQTRVK
VVKNKVAPPFKQAEFDIIYTEGISKLGEIIEIGVRNKIIEKAGSYYSYGDVKLGQGKENVKQFLKENKELAAELERKVRD
SMLLIAT

Sequences:

>Translated_327_residues
MNREDALKGVVVQIEKMFGKGSIMTFSQEPASVECISTGSIGLDSALGGGIPKGRIIEIFGPESSGKTTLTLHIIAEAQK
NGGTCAFIDAEHALDPVYARRLGVKIEELLISQPDTGEQALEIVDSLIKSGAVDVIVVDSVAALTPKAEIEGEMGDLHVG
LQARLLSQALRKITASVSRSNCVVVFINQIRMKIGVMFGSPETTTGGNALKFYSSVRIDIRKIGSIKDKEDVVGNQTRVK
VVKNKVAPPFKQAEFDIIYTEGISKLGEIIEIGVRNKIIEKAGSYYSYGDVKLGQGKENVKQFLKENKELAAELERKVRD
SMLLIAT
>Mature_327_residues
MNREDALKGVVVQIEKMFGKGSIMTFSQEPASVECISTGSIGLDSALGGGIPKGRIIEIFGPESSGKTTLTLHIIAEAQK
NGGTCAFIDAEHALDPVYARRLGVKIEELLISQPDTGEQALEIVDSLIKSGAVDVIVVDSVAALTPKAEIEGEMGDLHVG
LQARLLSQALRKITASVSRSNCVVVFINQIRMKIGVMFGSPETTTGGNALKFYSSVRIDIRKIGSIKDKEDVVGNQTRVK
VVKNKVAPPFKQAEFDIIYTEGISKLGEIIEIGVRNKIIEKAGSYYSYGDVKLGQGKENVKQFLKENKELAAELERKVRD
SMLLIAT

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Homo sapiens, GI23238219, Length=219, Percent_Identity=28.310502283105, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789051, Length=324, Percent_Identity=66.9753086419753, Blast_Score=451, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24651285, Length=262, Percent_Identity=28.2442748091603, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17864108, Length=226, Percent_Identity=30.0884955752212, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 35338; Mature: 35338

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREDALKGVVVQIEKMFGKGSIMTFSQEPASVECISTGSIGLDSALGGGIPKGRIIEIF
CCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCHHHCCCCCCCCEEEEE
GPESSGKTTLTLHIIAEAQKNGGTCAFIDAEHALDPVYARRLGVKIEELLISQPDTGEQA
CCCCCCCEEEEEEEEEEECCCCCEEEEEEHHHCCCHHHHHHHCCCHHHHHCCCCCCHHHH
LEIVDSLIKSGAVDVIVVDSVAALTPKAEIEGEMGDLHVGLQARLLSQALRKITASVSRS
HHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCC
NCVVVFINQIRMKIGVMFGSPETTTGGNALKFYSSVRIDIRKIGSIKDKEDVVGNQTRVK
CEEEEEEEHEEEEEEEEECCCCCCCCCCEEEEHHHHEEEEEHHCCCCCCHHHCCCCHHHH
VVKNKVAPPFKQAEFDIIYTEGISKLGEIIEIGVRNKIIEKAGSYYSYGDVKLGQGKENV
EEHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCHHHH
KQFLKENKELAAELERKVRDSMLLIAT
HHHHHHHHHHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MNREDALKGVVVQIEKMFGKGSIMTFSQEPASVECISTGSIGLDSALGGGIPKGRIIEIF
CCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCHHHCCCCCCCCEEEEE
GPESSGKTTLTLHIIAEAQKNGGTCAFIDAEHALDPVYARRLGVKIEELLISQPDTGEQA
CCCCCCCEEEEEEEEEEECCCCCEEEEEEHHHCCCHHHHHHHCCCHHHHHCCCCCCHHHH
LEIVDSLIKSGAVDVIVVDSVAALTPKAEIEGEMGDLHVGLQARLLSQALRKITASVSRS
HHHHHHHHHCCCEEEEEECCHHHHCCCHHCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCC
NCVVVFINQIRMKIGVMFGSPETTTGGNALKFYSSVRIDIRKIGSIKDKEDVVGNQTRVK
CEEEEEEEHEEEEEEEEECCCCCCCCCCEEEEHHHHEEEEEHHCCCCCCHHHCCCCHHHH
VVKNKVAPPFKQAEFDIIYTEGISKLGEIIEIGVRNKIIEKAGSYYSYGDVKLGQGKENV
EEHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEECCCHHHH
KQFLKENKELAAELERKVRDSMLLIAT
HHHHHHHHHHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA