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The map label for this gene is purM

Identifier: 88607996

GI number: 88607996

Start: 25144

End: 26112

Strand: Reverse

Name: purM

Synonym: NSE_0031

Alternate gene names: 88607996

Gene position: 26112-25144 (Counterclockwise)

Preceding gene: 88608111

Following gene: 88608507

Centisome position: 3.04

GC content: 42.62

Gene sequence:

>969_bases
ATGAAAAAAACTCTCTCCTACAAAAGCTCCGGTGTGAATATAGATACCGCGAACAGCTTTGTTGATTTTATTAAAAAGAA
CGCAGAGGTTGATAAGCACTGCGTTTCCGCTATTGGTGGGTTTGCTTCTTTATTCTCCGTAGGCAATTTGAATTACAAGG
ACCCTGTAATTGCTGCAGCAACAGACGGTGTTGGAACTAAGCTATTGATAGCAAATGAGTGCAATAACCATGCTAGTATT
GGTATAGACCTAGTAGCCATGTGCGCCAACGACCTGATCTGTCACGGGGCAAAACCGCTCTTTTTTCTTGATTATTACTC
GACAGGGAAACTTGAGCTCGATGTAGCACGCGCAGTAATCTCAGGGATTCTAGTCGGGTGTAGAGAGGCTTCCATGTCAC
TGGTAGGGGGAGAAACCGCTGAAATGCCCGGCTTATACAGTGCTGGTGAATACGATTTAGCCGGCTTTGCTGTCGGAATA
GTTGAAAAAGAGGAAATTCTCCCACAAAATGTCACGAAAGGTGACATCCTTATTGGGCTGAAATCATCAGGCTTTCATGC
GAATGGATTCTCACTTATCCGCAAAACTTTCTCCAGCTTAAGTATTAAGTACTCAACACTGTTCGATAAAAGAACCTGGG
GTGAGATACTCCTAACCCCAACAAAAATATATGTGAATTCGTTTCTTTCTTTGAAAAAATTCATAAAGGCGGCTGCACAC
ATCACGGGTGGCGGACTACTAGAAAATCTTAGAAGGGTTCTTCCAGAAGACCTAAAAATCTGTATTCAGCCTTATGAGTT
TCCCGAAATTTTCAAGTGGCTCATGCTGAACGGTAATATTCCGCAAGAAGAAATGTTAACTACTTTTAACTGTGGCATAG
GAATGGTTTTGGTTGTCGCGGAGCAAAATGCTGAGTTTGTTTCCGAGACACTAGGAGAGGAAGCTCTAATTATCGGAAAC
CTTAAGTAG

Upstream 100 bases:

>100_bases
AGTGGAAACATCGCTAGAAGTTGTGATACAATCGCGGTCTACCTGCACTCATATACAGTTATTACCGCAGCACCTTTTCA
GCGGTGAGTTCTGCCTGAAA

Downstream 100 bases:

>100_bases
GATTTCAGCAAACCAAGCCCCGGAGTTTACCCTTGCCTTTTAGATTACGCTTCCCTGAAATATTCACTCAGTTCCCTTGG
ACCAGGATAGATTTTTGTCA

Product: phosphoribosylformylglycinamidine cyclo-ligase

Products: NA

Alternate protein names: AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKKTLSYKSSGVNIDTANSFVDFIKKNAEVDKHCVSAIGGFASLFSVGNLNYKDPVIAAATDGVGTKLLIANECNNHASI
GIDLVAMCANDLICHGAKPLFFLDYYSTGKLELDVARAVISGILVGCREASMSLVGGETAEMPGLYSAGEYDLAGFAVGI
VEKEEILPQNVTKGDILIGLKSSGFHANGFSLIRKTFSSLSIKYSTLFDKRTWGEILLTPTKIYVNSFLSLKKFIKAAAH
ITGGGLLENLRRVLPEDLKICIQPYEFPEIFKWLMLNGNIPQEEMLTTFNCGIGMVLVVAEQNAEFVSETLGEEALIIGN
LK

Sequences:

>Translated_322_residues
MKKTLSYKSSGVNIDTANSFVDFIKKNAEVDKHCVSAIGGFASLFSVGNLNYKDPVIAAATDGVGTKLLIANECNNHASI
GIDLVAMCANDLICHGAKPLFFLDYYSTGKLELDVARAVISGILVGCREASMSLVGGETAEMPGLYSAGEYDLAGFAVGI
VEKEEILPQNVTKGDILIGLKSSGFHANGFSLIRKTFSSLSIKYSTLFDKRTWGEILLTPTKIYVNSFLSLKKFIKAAAH
ITGGGLLENLRRVLPEDLKICIQPYEFPEIFKWLMLNGNIPQEEMLTTFNCGIGMVLVVAEQNAEFVSETLGEEALIIGN
LK
>Mature_322_residues
MKKTLSYKSSGVNIDTANSFVDFIKKNAEVDKHCVSAIGGFASLFSVGNLNYKDPVIAAATDGVGTKLLIANECNNHASI
GIDLVAMCANDLICHGAKPLFFLDYYSTGKLELDVARAVISGILVGCREASMSLVGGETAEMPGLYSAGEYDLAGFAVGI
VEKEEILPQNVTKGDILIGLKSSGFHANGFSLIRKTFSSLSIKYSTLFDKRTWGEILLTPTKIYVNSFLSLKKFIKAAAH
ITGGGLLENLRRVLPEDLKICIQPYEFPEIFKWLMLNGNIPQEEMLTTFNCGIGMVLVVAEQNAEFVSETLGEEALIIGN
LK

Specific function: De novo purine biosynthesis; fifth step. [C]

COG id: COG0150

COG function: function code F; Phosphoribosylaminoimidazole (AIR) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AIR synthase family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=334, Percent_Identity=48.8023952095808, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI209869995, Length=334, Percent_Identity=48.8023952095808, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI209869993, Length=334, Percent_Identity=48.8023952095808, Blast_Score=322, Evalue=3e-88,
Organism=Escherichia coli, GI1788845, Length=332, Percent_Identity=47.5903614457831, Blast_Score=295, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17567511, Length=324, Percent_Identity=45.679012345679, Blast_Score=255, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321203, Length=334, Percent_Identity=44.0119760479042, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24582400, Length=283, Percent_Identity=46.2897526501767, Blast_Score=244, Evalue=5e-65,

Paralogues:

None

Copy number: 180 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR004733
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =6.3.3.1 [H]

Molecular weight: Translated: 34854; Mature: 34854

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLSYKSSGVNIDTANSFVDFIKKNAEVDKHCVSAIGGFASLFSVGNLNYKDPVIAAA
CCCCCCCCCCCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
TDGVGTKLLIANECNNHASIGIDLVAMCANDLICHGAKPLFFLDYYSTGKLELDVARAVI
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHH
SGILVGCREASMSLVGGETAEMPGLYSAGEYDLAGFAVGIVEKEEILPQNVTKGDILIGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCEEEEE
KSSGFHANGFSLIRKTFSSLSIKYSTLFDKRTWGEILLTPTKIYVNSFLSLKKFIKAAAH
ECCCCCCCHHHHHHHHHHHCEEEEHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHH
ITGGGLLENLRRVLPEDLKICIQPYEFPEIFKWLMLNGNIPQEEMLTTFNCGIGMVLVVA
CCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEE
EQNAEFVSETLGEEALIIGNLK
CCCHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure
MKKTLSYKSSGVNIDTANSFVDFIKKNAEVDKHCVSAIGGFASLFSVGNLNYKDPVIAAA
CCCCCCCCCCCCCEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
TDGVGTKLLIANECNNHASIGIDLVAMCANDLICHGAKPLFFLDYYSTGKLELDVARAVI
CCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHH
SGILVGCREASMSLVGGETAEMPGLYSAGEYDLAGFAVGIVEKEEILPQNVTKGDILIGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCEEEEE
KSSGFHANGFSLIRKTFSSLSIKYSTLFDKRTWGEILLTPTKIYVNSFLSLKKFIKAAAH
ECCCCCCCHHHHHHHHHHHCEEEEHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHH
ITGGGLLENLRRVLPEDLKICIQPYEFPEIFKWLMLNGNIPQEEMLTTFNCGIGMVLVVA
CCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEE
EQNAEFVSETLGEEALIIGNLK
CCCHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA