Click here to switch to the map view.

The map label for this gene is ribB [H]

Identifier: 88607875

GI number: 88607875

Start: 1398549

End: 1399178

Strand: Reverse

Name: ribB [H]

Synonym: APH_1332

Alternate gene names: 88607875

Gene position: 1399178-1398549 (Counterclockwise)

Preceding gene: 88607617

Following gene: 88607027

Centisome position: 95.1

GC content: 46.98

Gene sequence:

>630_bases
ATGAGTTTTGAGCCGAGTGCGGCTGTCTTTTCTTCCATTGAAGAAGTTGTTAGGGATGCTGCGGAAGGGAAGGTGTTTGT
GCTTCTCGATGACGAGGAGAGGGAGAACGAGGGTGATTTGGTAGCTTTAGCGGATAGGGTGAGTCCTGAGGCTATCAATA
TGATGATCAGATATGGAAGTGGGATAGTGTGTCTTGCATTTTCTGAGTATCACGCTAAGAAGCTTGGTTTGGGTTTGATG
TCTCGTAGGAATGCGAATGATGGTTGTCCTGCCTTTGCTGAATCTATTGATGCTAGGCATGGAATAACTACGGGTGTATC
TGCGAGTGACAGGGCTGCTACTATATTGAAGGCTGTATCTGATGACAGTAGTGCTGATGATATAGTCACGCCTGGGCATG
TCTTTCCTATAGTGGCGAATCCTGGTGGTGTTTTGAAGAGGGGTGGTCACACTGAGGCTGGTGTAGACATTGCCAAATTG
GCAGGTGCGGCTCATGCCGCTGTTATCTGTGAGCTTATGAATCCGGATGGTACTATGGCGCGATTGCCTGAAATCATGGA
GTTTGCAAGTGCAAATGGGATTAAGGTGACTACGATTAAGAAGCTGAGAGAATATCTTAGTAGCTCCTGA

Upstream 100 bases:

>100_bases
GCAAGCGAGAAGGTGACAGTGTTGAGCTGAAAACGCCGCGTGGAGACTATAAGGTATATCAAATTAACAAAGTTGAGTTC
ATATAGGTATTGGGTTTAGT

Downstream 100 bases:

>100_bases
CGCATGTATGGATATTGTAGCGGAAAATCGGAAGGTTAGGTTTAATTACACTATTCTCAGTGAGTATGATGCTGGTATTG
TGTTGCTAGGTAGTGAGGTG

Product: 3,4-dihydroxy-2-butanone 4-phosphate synthase

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSFEPSAAVFSSIEEVVRDAAEGKVFVLLDDEERENEGDLVALADRVSPEAINMMIRYGSGIVCLAFSEYHAKKLGLGLM
SRRNANDGCPAFAESIDARHGITTGVSASDRAATILKAVSDDSSADDIVTPGHVFPIVANPGGVLKRGGHTEAGVDIAKL
AGAAHAAVICELMNPDGTMARLPEIMEFASANGIKVTTIKKLREYLSSS

Sequences:

>Translated_209_residues
MSFEPSAAVFSSIEEVVRDAAEGKVFVLLDDEERENEGDLVALADRVSPEAINMMIRYGSGIVCLAFSEYHAKKLGLGLM
SRRNANDGCPAFAESIDARHGITTGVSASDRAATILKAVSDDSSADDIVTPGHVFPIVANPGGVLKRGGHTEAGVDIAKL
AGAAHAAVICELMNPDGTMARLPEIMEFASANGIKVTTIKKLREYLSSS
>Mature_208_residues
SFEPSAAVFSSIEEVVRDAAEGKVFVLLDDEERENEGDLVALADRVSPEAINMMIRYGSGIVCLAFSEYHAKKLGLGLMS
RRNANDGCPAFAESIDARHGITTGVSASDRAATILKAVSDDSSADDIVTPGHVFPIVANPGGVLKRGGHTEAGVDIAKLA
GAAHAAVICELMNPDGTMARLPEIMEFASANGIKVTTIKKLREYLSSS

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=196, Percent_Identity=45.9183673469388, Blast_Score=179, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6320695, Length=206, Percent_Identity=38.8349514563107, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 22007; Mature: 21876

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEPSAAVFSSIEEVVRDAAEGKVFVLLDDEERENEGDLVALADRVSPEAINMMIRYGS
CCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCC
GIVCLAFSEYHAKKLGLGLMSRRNANDGCPAFAESIDARHGITTGVSASDRAATILKAVS
CEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
DDSSADDIVTPGHVFPIVANPGGVLKRGGHTEAGVDIAKLAGAAHAAVICELMNPDGTMA
CCCCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHHHHCHHHHHHEEEEECCCCHHH
RLPEIMEFASANGIKVTTIKKLREYLSSS
HHHHHHHHHCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure 
SFEPSAAVFSSIEEVVRDAAEGKVFVLLDDEERENEGDLVALADRVSPEAINMMIRYGS
CCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCC
GIVCLAFSEYHAKKLGLGLMSRRNANDGCPAFAESIDARHGITTGVSASDRAATILKAVS
CEEEEEECHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHC
DDSSADDIVTPGHVFPIVANPGGVLKRGGHTEAGVDIAKLAGAAHAAVICELMNPDGTMA
CCCCCCCCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHHHHCHHHHHHEEEEECCCCHHH
RLPEIMEFASANGIKVTTIKKLREYLSSS
HHHHHHHHHCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]