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The map label for this gene is lspA

Identifier: 88607803

GI number: 88607803

Start: 1225809

End: 1226279

Strand: Reverse

Name: lspA

Synonym: APH_1160

Alternate gene names: 88607803

Gene position: 1226279-1225809 (Counterclockwise)

Preceding gene: 88607389

Following gene: 88607658

Centisome position: 83.35

GC content: 39.7

Gene sequence:

>471_bases
ATGAGGAAATCTATAATCGGAATAATAGTGCTGCACGTTGTGATGGCATTAGATCAGATAAGCAAGTTGTATATGTCCAA
GCTGTACGCGGCACACGGCGATATTACTGTTTTTGAATATTGTAATTTGATACAGCTTTGGAATAAAGGAATAAGCTTCG
GCCTGTTTAGTACCTTGGAAAATGGAAACACGGTTTTTATGGTATTATCAGCAGTCATTATAGCAATCCTGTCATATACG
AAGATCAAAACGAAAAGTATGTCAAGAAGTTGCTGTTTGAGCGTTATAGTGGGCGGTGCGCTTGGGAATTTAATGGATAG
ACTAAGATTCGGTGCTGTGTATGACTTCATAGATTTACACATTGGAGATTGGCACTGGCCTGCGTTTAACTTGGCAGATC
TCACTATAACGTGTGGAGTGATTGTCTTTTTAGCTATGGAACTCAGAAAACGCAGCCAGCTTAATGCTTAG

Upstream 100 bases:

>100_bases
ACACTAGTCATAATACATATATTGTGCCAATAGAGTTCTTCTATTCAAAGAAAATTGTTTCTGCTATTATGAGCGCGACA
ATGATACTGTAGGTTGTGTT

Downstream 100 bases:

>100_bases
GAAACAAGGGATAGTTCATGATATTGTTAAGATAGTTGAAACTATTTACGGTAATATTTCGGATAAAGTTGTTTCATTTT
TGGATGAAAAAAAGGATGAG

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MRKSIIGIIVLHVVMALDQISKLYMSKLYAAHGDITVFEYCNLIQLWNKGISFGLFSTLENGNTVFMVLSAVIIAILSYT
KIKTKSMSRSCCLSVIVGGALGNLMDRLRFGAVYDFIDLHIGDWHWPAFNLADLTITCGVIVFLAMELRKRSQLNA

Sequences:

>Translated_156_residues
MRKSIIGIIVLHVVMALDQISKLYMSKLYAAHGDITVFEYCNLIQLWNKGISFGLFSTLENGNTVFMVLSAVIIAILSYT
KIKTKSMSRSCCLSVIVGGALGNLMDRLRFGAVYDFIDLHIGDWHWPAFNLADLTITCGVIVFLAMELRKRSQLNA
>Mature_156_residues
MRKSIIGIIVLHVVMALDQISKLYMSKLYAAHGDITVFEYCNLIQLWNKGISFGLFSTLENGNTVFMVLSAVIIAILSYT
KIKTKSMSRSCCLSVIVGGALGNLMDRLRFGAVYDFIDLHIGDWHWPAFNLADLTITCGVIVFLAMELRKRSQLNA

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=146, Percent_Identity=33.5616438356164, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_ANAPZ (Q2GIV1)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505700.1
- STRING:   Q2GIV1
- MEROPS:   A08.001
- GeneID:   3930846
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_1160
- NMPDR:   fig|212042.5.peg.1112
- TIGR:   APH_1160
- eggNOG:   COG0597
- HOGENOM:   HBG724422
- OMA:   GLFAQYK
- PhylomeDB:   Q2GIV1
- ProtClustDB:   PRK14775
- BioCyc:   APHA212042:APH_1160-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 17424; Mature: 17424

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 106-106 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

HASH(0x409a5b2c)-; HASH(0x17565574)-;

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSIIGIIVLHVVMALDQISKLYMSKLYAAHGDITVFEYCNLIQLWNKGISFGLFSTLE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHC
NGNTVFMVLSAVIIAILSYTKIKTKSMSRSCCLSVIVGGALGNLMDRLRFGAVYDFIDLH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IGDWHWPAFNLADLTITCGVIVFLAMELRKRSQLNA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MRKSIIGIIVLHVVMALDQISKLYMSKLYAAHGDITVFEYCNLIQLWNKGISFGLFSTLE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHC
NGNTVFMVLSAVIIAILSYTKIKTKSMSRSCCLSVIVGGALGNLMDRLRFGAVYDFIDLH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IGDWHWPAFNLADLTITCGVIVFLAMELRKRSQLNA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA