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The map label for this gene is nrdF [H]

Identifier: 88607783

GI number: 88607783

Start: 588436

End: 589407

Strand: Direct

Name: nrdF [H]

Synonym: APH_0560

Alternate gene names: 88607783

Gene position: 588436-589407 (Clockwise)

Preceding gene: 88606779

Following gene: 88607059

Centisome position: 39.99

GC content: 43.42

Gene sequence:

>972_bases
ATGTCGCTGTTAGATGCTAAGCCTGTATACAAGCCTTTTGACTATCCATGGGCGTATGATGCATGGTTATGCCAACAGAG
GATTCATTGGTTGCCCGAAGAAGTGCCTCTTGCTGATGATGTGCAGGATTGGAAGACCATGCTCTCTGAGAAAGAGAAGA
ATCTCCTCGTGCAGATTTTCCGTTTTTTTACTCAGGCGGATATAGAGGTGAATAATTGCTATATGAAGCACTATTCTAAT
ATCTTCAGACCCACAGAGATATGCATGATGCTGTCTGCGTTTTCCAATATGGAGACCATACACGTGGCTGCATATTCGTA
CTTGCTAGATACTATTGGGATGCCTGAGGTAGAATATCAGGCGTTTTTGAAGTTTGAGGTCATGAAGAAGAAATATGACT
ACATGCAGAGGTTTGAAGAGTGCAGAAGGGGCGATAAGAGACATGTAGCTAAGACTCTTGCTGTGTTTGGTGCGTTTACA
GAAGGGTTGCAGTTATTTGCTTCTTTCGCGATTTTGCTAAATTTTCAACGTTTCGGCAAGATGAAGGGGATGGGGCAGAT
TATAGCTTGGTCTGCGCGTGATGAGAGTTTGCATACAGATTCTATCATAAGGTTATTCAACACCTTCGTCCACGAAAACG
GTGAGATATGGGACGATGATCTGAAACAAGAGCTTTACGATGCGTGTAAAGTTATAGTAATGCTCGAGGATGAGTTTATA
GACCTTGCGTTTGCTTTAGGTGATGTTGAGGGTCTTTCCGCGTGTGAAGTGAAGAGGTATATACGCTTCATAGCCAATAG
AAGATTGAAGCAGCTGCACTTGGAGCCTATTTATGATGTTGCGGAGAACCCGTTACCATGGTTAGATGAGATTTTAAACG
GGGTAGAGCATACAAACTTCTTCGAGAATAGGGTTACAGAATATGCTAGAGCTGCAACGGAGGGAACCTGGGAGGAAGCG
TTTGAGGATTAG

Upstream 100 bases:

>100_bases
TTGATACTAAGAATATAGCACGAAGGTTGTGGTCTGAGATTGACAATTTTCACACTTATTCGTAAGGTTTAGCCGTGAAC
TTTTTCTGGGGGTAGCGAGC

Downstream 100 bases:

>100_bases
CAAGTGGCTTACATGTGCTGTAGCTGCATTGCTTGTACTTTCGTGTTATGCGCACGCTGCTGAGCAAGTTTTGCAACACA
GGCGTGTTCAGGAGATATTA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSLLDAKPVYKPFDYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTMLSEKEKNLLVQIFRFFTQADIEVNNCYMKHYSN
IFRPTEICMMLSAFSNMETIHVAAYSYLLDTIGMPEVEYQAFLKFEVMKKKYDYMQRFEECRRGDKRHVAKTLAVFGAFT
EGLQLFASFAILLNFQRFGKMKGMGQIIAWSARDESLHTDSIIRLFNTFVHENGEIWDDDLKQELYDACKVIVMLEDEFI
DLAFALGDVEGLSACEVKRYIRFIANRRLKQLHLEPIYDVAENPLPWLDEILNGVEHTNFFENRVTEYARAATEGTWEEA
FED

Sequences:

>Translated_323_residues
MSLLDAKPVYKPFDYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTMLSEKEKNLLVQIFRFFTQADIEVNNCYMKHYSN
IFRPTEICMMLSAFSNMETIHVAAYSYLLDTIGMPEVEYQAFLKFEVMKKKYDYMQRFEECRRGDKRHVAKTLAVFGAFT
EGLQLFASFAILLNFQRFGKMKGMGQIIAWSARDESLHTDSIIRLFNTFVHENGEIWDDDLKQELYDACKVIVMLEDEFI
DLAFALGDVEGLSACEVKRYIRFIANRRLKQLHLEPIYDVAENPLPWLDEILNGVEHTNFFENRVTEYARAATEGTWEEA
FED
>Mature_322_residues
SLLDAKPVYKPFDYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTMLSEKEKNLLVQIFRFFTQADIEVNNCYMKHYSNI
FRPTEICMMLSAFSNMETIHVAAYSYLLDTIGMPEVEYQAFLKFEVMKKKYDYMQRFEECRRGDKRHVAKTLAVFGAFTE
GLQLFASFAILLNFQRFGKMKGMGQIIAWSARDESLHTDSIIRLFNTFVHENGEIWDDDLKQELYDACKVIVMLEDEFID
LAFALGDVEGLSACEVKRYIRFIANRRLKQLHLEPIYDVAENPLPWLDEILNGVEHTNFFENRVTEYARAATEGTWEEAF
ED

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI4557845, Length=307, Percent_Identity=30.2931596091205, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI260064013, Length=307, Percent_Identity=30.2931596091205, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI42544136, Length=307, Percent_Identity=30.2931596091205, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI289177074, Length=307, Percent_Identity=30.2931596091205, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI289177076, Length=286, Percent_Identity=30.4195804195804, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1789031, Length=294, Percent_Identity=25.5102040816327, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17551968, Length=289, Percent_Identity=30.4498269896194, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322434, Length=328, Percent_Identity=26.8292682926829, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18079279, Length=305, Percent_Identity=27.8688524590164, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38048; Mature: 37917

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLDAKPVYKPFDYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTMLSEKEKNLLVQIF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RFFTQADIEVNNCYMKHYSNIFRPTEICMMLSAFSNMETIHVAAYSYLLDTIGMPEVEYQ
HHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHH
AFLKFEVMKKKYDYMQRFEECRRGDKRHVAKTLAVFGAFTEGLQLFASFAILLNFQRFGK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKGMGQIIAWSARDESLHTDSIIRLFNTFVHENGEIWDDDLKQELYDACKVIVMLEDEFI
HCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEHHHHH
DLAFALGDVEGLSACEVKRYIRFIANRRLKQLHLEPIYDVAENPLPWLDEILNGVEHTNF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHH
FENRVTEYARAATEGTWEEAFED
HHHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure 
SLLDAKPVYKPFDYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTMLSEKEKNLLVQIF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]