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The map label for this gene is purB [H]

Identifier: 88607713

GI number: 88607713

Start: 921530

End: 922828

Strand: Direct

Name: purB [H]

Synonym: APH_0867

Alternate gene names: 88607713

Gene position: 921530-922828 (Clockwise)

Preceding gene: 88607290

Following gene: 88607470

Centisome position: 62.63

GC content: 42.96

Gene sequence:

>1299_bases
GTGATCGCAAGATATAGTATCCCCGAAATGGTGGAAATTTGGGAAGAAGAAAACAAGTATAGGATATGGTTCGCCATAGA
AAAAAGTGCGTGTGAAGCACAGGTTAAGCTAGGTGTTGTGCCAGTCCATGTTTTGGATAGCCTAAAGTTAGTTGACGATA
GAAAGTTTGATATCAAGCGGATTTGTGAAATAGAAGCCGAGGTGAAACATGATGTTATAGCTTTTCTATCATATATCGCA
GAATCCACTTCCACGGATATACGGTATCTTCACTATGGAATGACTAGCTCCGACGTTCTGGATACTTGCTTATCGTTCCA
GCTTAAGCAATCATGTGATATTTTGCTAAAAAACATAGAAGACGTCCTTGGTATCCTCAAAAGAAGATCAGAAGAGACGA
AATACCTTCTCTGTATGGGTAGAAGCCACGGTGTTCACGCAGAGCCCATAACGTTCGGCTTGAAGCTTGCCAGGTTTTAT
GCGGAGTTTGAGCGCAACCGTCAACGCTTGATAAGAGCACGTGAAGAGATATCTGTATGTAAAATCTCCGGAGCTATGGG
GAATTTTGCGCATCTTGATCCATATGTTGAGGAATATGTGGGAAATTCTCTTGGGCTAAAGCCTGAGACCATATCTTCTC
AAGTCATATCCAGAGATCGCTATGCTGTATTTTTTGCCACTTTAGGCGTTATAGCTTCTTCAATGGAGAAAATAGCTACC
GAGATTAGGCACCTACAGCAGACAGAGGTTCTTGAAGTAACCGAGCCATTCTCACAAGGTCAGAAGGGTAGTTCTGCGAT
GCCGCATAAGCAGAATCCGATCCTTGGTGAAAACTTGACAGGTCTGTCACGGATGATCAGAAGTTATGTTGTACCTGCTA
TGGAAAATGTTGCGTTATGGCACGAAAGGGATATCTCCCACTCTTCTGTTGAGAGATTCATAGCCCCGGATGCCTGCGTG
ACGCTTAATTTTGCGCTGGTTAGGTTAGCTCACCTTCTCCAAAATATGTGTATAAACGAAGAGAGGATCAGGGCAAATCT
TGATTCGTCTAAGAATATGATGCTGTCACAGCGTGTCTTACTCGCGCTTGTCGATACCGGGATGAGTAGGGAGCAAGGAT
ACAAAATTGTGCAAGACTGCGCTATGGATAGCTGGAAAAATGGCACTGACTTTGTTGAAAACATCAGTAATCATCCTCAG
GCGAGAGATTGCTTAAAGGATATAGGGGAACTTGCTGACCTGAGCTACTATACAAAGCATGTAGACTTTATTTTTAATAA
GGTATTTGGCACAGAGTAA

Upstream 100 bases:

>100_bases
CACAATGCACTAGAGGTGTTACAATACTACACTTTTACTTATTGCAAAGCAGAAGCATATATTGCATCATTCCCGGCAGT
AGAGTTTGTGAAGTACTTCT

Downstream 100 bases:

>100_bases
AGTATCACCCACCATGTTCCTAGCAAGCACATACGAGCCTAGGGACTACTGTATGAGGGTACGCGTATTGTGTGCCCTTA
AACACCTGTGGGGCGCAAAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIARYSIPEMVEIWEEENKYRIWFAIEKSACEAQVKLGVVPVHVLDSLKLVDDRKFDIKRICEIEAEVKHDVIAFLSYIA
ESTSTDIRYLHYGMTSSDVLDTCLSFQLKQSCDILLKNIEDVLGILKRRSEETKYLLCMGRSHGVHAEPITFGLKLARFY
AEFERNRQRLIRAREEISVCKISGAMGNFAHLDPYVEEYVGNSLGLKPETISSQVISRDRYAVFFATLGVIASSMEKIAT
EIRHLQQTEVLEVTEPFSQGQKGSSAMPHKQNPILGENLTGLSRMIRSYVVPAMENVALWHERDISHSSVERFIAPDACV
TLNFALVRLAHLLQNMCINEERIRANLDSSKNMMLSQRVLLALVDTGMSREQGYKIVQDCAMDSWKNGTDFVENISNHPQ
ARDCLKDIGELADLSYYTKHVDFIFNKVFGTE

Sequences:

>Translated_432_residues
MIARYSIPEMVEIWEEENKYRIWFAIEKSACEAQVKLGVVPVHVLDSLKLVDDRKFDIKRICEIEAEVKHDVIAFLSYIA
ESTSTDIRYLHYGMTSSDVLDTCLSFQLKQSCDILLKNIEDVLGILKRRSEETKYLLCMGRSHGVHAEPITFGLKLARFY
AEFERNRQRLIRAREEISVCKISGAMGNFAHLDPYVEEYVGNSLGLKPETISSQVISRDRYAVFFATLGVIASSMEKIAT
EIRHLQQTEVLEVTEPFSQGQKGSSAMPHKQNPILGENLTGLSRMIRSYVVPAMENVALWHERDISHSSVERFIAPDACV
TLNFALVRLAHLLQNMCINEERIRANLDSSKNMMLSQRVLLALVDTGMSREQGYKIVQDCAMDSWKNGTDFVENISNHPQ
ARDCLKDIGELADLSYYTKHVDFIFNKVFGTE
>Mature_432_residues
MIARYSIPEMVEIWEEENKYRIWFAIEKSACEAQVKLGVVPVHVLDSLKLVDDRKFDIKRICEIEAEVKHDVIAFLSYIA
ESTSTDIRYLHYGMTSSDVLDTCLSFQLKQSCDILLKNIEDVLGILKRRSEETKYLLCMGRSHGVHAEPITFGLKLARFY
AEFERNRQRLIRAREEISVCKISGAMGNFAHLDPYVEEYVGNSLGLKPETISSQVISRDRYAVFFATLGVIASSMEKIAT
EIRHLQQTEVLEVTEPFSQGQKGSSAMPHKQNPILGENLTGLSRMIRSYVVPAMENVALWHERDISHSSVERFIAPDACV
TLNFALVRLAHLLQNMCINEERIRANLDSSKNMMLSQRVLLALVDTGMSREQGYKIVQDCAMDSWKNGTDFVENISNHPQ
ARDCLKDIGELADLSYYTKHVDFIFNKVFGTE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=396, Percent_Identity=25.2525252525253, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI183227688, Length=387, Percent_Identity=25.0645994832041, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1787376, Length=356, Percent_Identity=26.123595505618, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508577, Length=389, Percent_Identity=26.4781491002571, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564234, Length=334, Percent_Identity=29.3413173652695, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=391, Percent_Identity=25.5754475703325, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647570, Length=436, Percent_Identity=22.4770642201835, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49235; Mature: 49235

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARYSIPEMVEIWEEENKYRIWFAIEKSACEAQVKLGVVPVHVLDSLKLVDDRKFDIKR
CCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHH
ICEIEAEVKHDVIAFLSYIAESTSTDIRYLHYGMTSSDVLDTCLSFQLKQSCDILLKNIE
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
DVLGILKRRSEETKYLLCMGRSHGVHAEPITFGLKLARFYAEFERNRQRLIRAREEISVC
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KISGAMGNFAHLDPYVEEYVGNSLGLKPETISSQVISRDRYAVFFATLGVIASSMEKIAT
EECCCCCCCHHCCHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EIRHLQQTEVLEVTEPFSQGQKGSSAMPHKQNPILGENLTGLSRMIRSYVVPAMENVALW
HHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HERDISHSSVERFIAPDACVTLNFALVRLAHLLQNMCINEERIRANLDSSKNMMLSQRVL
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHH
LALVDTGMSREQGYKIVQDCAMDSWKNGTDFVENISNHPQARDCLKDIGELADLSYYTKH
HHHHHCCCCHHHCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VDFIFNKVFGTE
HHHHHHHHHCCC
>Mature Secondary Structure
MIARYSIPEMVEIWEEENKYRIWFAIEKSACEAQVKLGVVPVHVLDSLKLVDDRKFDIKR
CCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCHHHHCCCCHHHHHHHHHHHCCCCCCHHH
ICEIEAEVKHDVIAFLSYIAESTSTDIRYLHYGMTSSDVLDTCLSFQLKQSCDILLKNIE
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
DVLGILKRRSEETKYLLCMGRSHGVHAEPITFGLKLARFYAEFERNRQRLIRAREEISVC
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KISGAMGNFAHLDPYVEEYVGNSLGLKPETISSQVISRDRYAVFFATLGVIASSMEKIAT
EECCCCCCCHHCCHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EIRHLQQTEVLEVTEPFSQGQKGSSAMPHKQNPILGENLTGLSRMIRSYVVPAMENVALW
HHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HERDISHSSVERFIAPDACVTLNFALVRLAHLLQNMCINEERIRANLDSSKNMMLSQRVL
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHH
LALVDTGMSREQGYKIVQDCAMDSWKNGTDFVENISNHPQARDCLKDIGELADLSYYTKH
HHHHHCCCCHHHCHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
VDFIFNKVFGTE
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]