Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 88607636

GI number: 88607636

Start: 18109

End: 18600

Strand: Reverse

Name: ruvC

Synonym: APH_0018

Alternate gene names: 88607636

Gene position: 18600-18109 (Counterclockwise)

Preceding gene: 88607040

Following gene: 88607335

Centisome position: 1.26

GC content: 39.84

Gene sequence:

>492_bases
ATGCTTGTTTTTGGCCTTGATCCCGGTCTTAATTACACTGGTTGGGCTGTCGTACTCAAAGAACCGTCTGGTTCTCTTTC
TCTCCTCGATACTGGTACTATTTGTACTACCTCCTGTTCTGATCTCAATGATAAGCTCTTTTGTATCTTTTCTGGCCTTT
CGGCTATTATTCAGAAGTTTTCTGTATCTGTAGCTTCTATTGAAAATGTTTTTGTTAATTTAAACCCAAAGGCCTCTATG
TTCCTTTGTTATGCTAGGGCTGCTTCATTGCTTGCTTGTCTTTCATCTAATGTCAAAATATTTGAGTACTCTCCTACTAA
AATCAAAAAGTGTGTTTTCGGGAATGGTAGGGCTTCAAAGGAGCAGATTGCTTTTGTAGTTAGATCTTCTTTGGGTTTAT
CTGAAGACGCTTCTTTCAGCTCTCACGCTTCAGATGCTATTGCAGCAGCTCTATGCCATATCTTTTCAAGTGGGAATAGG
TACGGCATCTGA

Upstream 100 bases:

>100_bases
TGTCACATTGGCTTCGAATGCGCTGCCTGGTACTGGCACTTCGTTGACGTGGTCTGGTTGTTCTTATTCCTTTTCATGTA
CGTTATTAGTTCTTAGCATC

Downstream 100 bases:

>100_bases
TCTATATAACTTTCTTTTCCTCTCTTTTTATAAGCCTAACAATGTTTTCTCTGTGTTTGAACAAAATCAATATACTTTGC
ACCGTGTATAAGAGCGCTAC

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MLVFGLDPGLNYTGWAVVLKEPSGSLSLLDTGTICTTSCSDLNDKLFCIFSGLSAIIQKFSVSVASIENVFVNLNPKASM
FLCYARAASLLACLSSNVKIFEYSPTKIKKCVFGNGRASKEQIAFVVRSSLGLSEDASFSSHASDAIAAALCHIFSSGNR
YGI

Sequences:

>Translated_163_residues
MLVFGLDPGLNYTGWAVVLKEPSGSLSLLDTGTICTTSCSDLNDKLFCIFSGLSAIIQKFSVSVASIENVFVNLNPKASM
FLCYARAASLLACLSSNVKIFEYSPTKIKKCVFGNGRASKEQIAFVVRSSLGLSEDASFSSHASDAIAAALCHIFSSGNR
YGI
>Mature_163_residues
MLVFGLDPGLNYTGWAVVLKEPSGSLSLLDTGTICTTSCSDLNDKLFCIFSGLSAIIQKFSVSVASIENVFVNLNPKASM
FLCYARAASLLACLSSNVKIFEYSPTKIKKCVFGNGRASKEQIAFVVRSSLGLSEDASFSSHASDAIAAALCHIFSSGNR
YGI

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=153, Percent_Identity=32.0261437908497, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_ANAPZ (Q2GLU6)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504655.1
- ProteinModelPortal:   Q2GLU6
- SMR:   Q2GLU6
- STRING:   Q2GLU6
- GeneID:   3930900
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0018
- NMPDR:   fig|212042.5.peg.16
- TIGR:   APH_0018
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RLANIFT
- PhylomeDB:   Q2GLU6
- ProtClustDB:   CLSK747421
- BioCyc:   APHA212042:APH_0018-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17327; Mature: 17327

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVFGLDPGLNYTGWAVVLKEPSGSLSLLDTGTICTTSCSDLNDKLFCIFSGLSAIIQKF
CEEEECCCCCCCCEEEEEEECCCCCEEEEECCCEEECCCCCCCCCEEHHHHHHHHHHHHH
SVSVASIENVFVNLNPKASMFLCYARAASLLACLSSNVKIFEYSPTKIKKCVFGNGRASK
CCHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHEECCCCCCCH
EQIAFVVRSSLGLSEDASFSSHASDAIAAALCHIFSSGNRYGI
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLVFGLDPGLNYTGWAVVLKEPSGSLSLLDTGTICTTSCSDLNDKLFCIFSGLSAIIQKF
CEEEECCCCCCCCEEEEEEECCCCCEEEEECCCEEECCCCCCCCCEEHHHHHHHHHHHHH
SVSVASIENVFVNLNPKASMFLCYARAASLLACLSSNVKIFEYSPTKIKKCVFGNGRASK
CCHHEEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHEECCCCCCCH
EQIAFVVRSSLGLSEDASFSSHASDAIAAALCHIFSSGNRYGI
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA