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The map label for this gene is nth [H]

Identifier: 88607623

GI number: 88607623

Start: 952813

End: 953442

Strand: Reverse

Name: nth [H]

Synonym: APH_0897

Alternate gene names: 88607623

Gene position: 953442-952813 (Counterclockwise)

Preceding gene: 88606765

Following gene: 88607235

Centisome position: 64.8

GC content: 41.27

Gene sequence:

>630_bases
ATGCAGAAAGCTGATATAATTTTCAGTCGTTTCTTCAGCGATAATCCTCATCCGCGTATAGAGTTGCAATATCGTAATGA
GTTTACGTTGTTGGTTGCGATCGTGTTATCTGCGCGTACTACGGATGTTAGCGTAAATAAGATCACGGCCAAGTTATTTG
ATGTTGCTAATTCTGCGAAGAAAATGCTTGCTCTGGGGGAAAGTGGGCTAAAGCGCTATATAAACAGTATAGGTTTATAT
AACTCTAAGGCTAAGAATATAATTCAGTTAAGTGAGATAATTGAGAATACCTATGGTGGTACGATCCCTAGAGATTTTGA
TGCATTGACGGCTCTTCCAGGTGTAGGGCGGAAGAGTGCAAATGTGTTTTTAAATTCATGTTTGGGCGTACCTACTATTG
CGGTTGATACTCATGTATTCAGGGTGAGCAATAGGATAGGATTGGTACAAGAGAGCAGTGTTTTGGGTGTTGAGAAATCG
CTTGAAAAAGTTGTGCCGGAGAAGTGGAAGTTATATGCGCATCACTGGTTAGTTCTACATGGACGGTATGTGTGTAAAGC
GCGTACTCCTTTATGTGGCAAGTGCATAGTTAGTGATTTGTGTGCTAGCAAGAGTGTGTTGTTGCGATAG

Upstream 100 bases:

>100_bases
TGGTGTGTCAGCGTGGTCTCTACACAGGCCTTATGTGTATTATAGCTGGTCAAATGGTTTATATTGGGTGATAATCAAAT
TTGTTTCCTGCTAAAAGTAT

Downstream 100 bases:

>100_bases
AGTGTGAAATCTGCTCCTAGTAGTGTGAGAAGTGGGGAGCGTGAATACGTGGAGTATTGACTATTACATGTCTATGGTGC
TCTGTAATATGCACATAGGG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MQKADIIFSRFFSDNPHPRIELQYRNEFTLLVAIVLSARTTDVSVNKITAKLFDVANSAKKMLALGESGLKRYINSIGLY
NSKAKNIIQLSEIIENTYGGTIPRDFDALTALPGVGRKSANVFLNSCLGVPTIAVDTHVFRVSNRIGLVQESSVLGVEKS
LEKVVPEKWKLYAHHWLVLHGRYVCKARTPLCGKCIVSDLCASKSVLLR

Sequences:

>Translated_209_residues
MQKADIIFSRFFSDNPHPRIELQYRNEFTLLVAIVLSARTTDVSVNKITAKLFDVANSAKKMLALGESGLKRYINSIGLY
NSKAKNIIQLSEIIENTYGGTIPRDFDALTALPGVGRKSANVFLNSCLGVPTIAVDTHVFRVSNRIGLVQESSVLGVEKS
LEKVVPEKWKLYAHHWLVLHGRYVCKARTPLCGKCIVSDLCASKSVLLR
>Mature_209_residues
MQKADIIFSRFFSDNPHPRIELQYRNEFTLLVAIVLSARTTDVSVNKITAKLFDVANSAKKMLALGESGLKRYINSIGLY
NSKAKNIIQLSEIIENTYGGTIPRDFDALTALPGVGRKSANVFLNSCLGVPTIAVDTHVFRVSNRIGLVQESSVLGVEKS
LEKVVPEKWKLYAHHWLVLHGRYVCKARTPLCGKCIVSDLCASKSVLLR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=183, Percent_Identity=31.1475409836066, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1787920, Length=199, Percent_Identity=55.7788944723618, Blast_Score=233, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17554540, Length=184, Percent_Identity=34.2391304347826, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=191, Percent_Identity=25.130890052356, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550361, Length=185, Percent_Identity=32.972972972973, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23191; Mature: 23191

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKADIIFSRFFSDNPHPRIELQYRNEFTLLVAIVLSARTTDVSVNKITAKLFDVANSAK
CCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KMLALGESGLKRYINSIGLYNSKAKNIIQLSEIIENTYGGTIPRDFDALTALPGVGRKSA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHH
NVFLNSCLGVPTIAVDTHVFRVSNRIGLVQESSVLGVEKSLEKVVPEKWKLYAHHWLVLH
HHHHHHHCCCCEEEEHHEEEEECCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHEEEEEE
GRYVCKARTPLCGKCIVSDLCASKSVLLR
CEEEEECCCCCHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure
MQKADIIFSRFFSDNPHPRIELQYRNEFTLLVAIVLSARTTDVSVNKITAKLFDVANSAK
CCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KMLALGESGLKRYINSIGLYNSKAKNIIQLSEIIENTYGGTIPRDFDALTALPGVGRKSA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHH
NVFLNSCLGVPTIAVDTHVFRVSNRIGLVQESSVLGVEKSLEKVVPEKWKLYAHHWLVLH
HHHHHHHCCCCEEEEHHEEEEECCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHEEEEEE
GRYVCKARTPLCGKCIVSDLCASKSVLLR
CEEEEECCCCCHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274032; 9823893 [H]