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The map label for this gene is greA [H]

Identifier: 88607617

GI number: 88607617

Start: 1399193

End: 1399693

Strand: Reverse

Name: greA [H]

Synonym: APH_1333

Alternate gene names: 88607617

Gene position: 1399693-1399193 (Counterclockwise)

Preceding gene: 88606923

Following gene: 88607875

Centisome position: 95.13

GC content: 43.71

Gene sequence:

>501_bases
ATGAGCGACTATGTGACTACTAGGTTTCCTATTACGAAGGGGGGCTACCAGAAATTGGAGTCGGAGCTGGAAGCTCTAAA
GAGTGAGAGACCGAAGATTATTAAATCTATTTCTGATGCTAGGGAGTTGGGTGATCTTTCGGAGAATGCTGAGTACCATG
CTGCGAGAGAGCGTCAGGGTCTTGTAGAAAGCAAGATAATGGAGCTTGAGTCTAAGAAATCTAGGGCTGAAGTCATAGAA
GTATCAGAATTATCGGGTGATACGGTTATGTTTGGGGCAACTGTTACTGTATCCGTGTATGATGAGCAGAGTGATACTAC
TAGCGAGGTTAAGTATCAGATAGTTGGGGAATATGAGGCGGATGTTTCGAAGAATATGATATCAATTAAGTCGCCGTTGG
TCCTGGCTCTATTGGGCAAGCGAGAAGGTGACAGTGTTGAGCTGAAAACGCCGCGTGGAGACTATAAGGTATATCAAATT
AACAAAGTTGAGTTCATATAG

Upstream 100 bases:

>100_bases
GGCTTGCTTGTTGGAGGATGATGTACGAAGTAAGCTGTTGGAGGTTATCAGGGGGTTTGCGGTGAGTTTTTGACAGTGAA
TGTTAGAGATTAGGTACTGT

Downstream 100 bases:

>100_bases
GTATTGGGTTTAGTATGAGTTTTGAGCCGAGTGCGGCTGTCTTTTCTTCCATTGAAGAAGTTGTTAGGGATGCTGCGGAA
GGGAAGGTGTTTGTGCTTCT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSDYVTTRFPITKGGYQKLESELEALKSERPKIIKSISDARELGDLSENAEYHAARERQGLVESKIMELESKKSRAEVIE
VSELSGDTVMFGATVTVSVYDEQSDTTSEVKYQIVGEYEADVSKNMISIKSPLVLALLGKREGDSVELKTPRGDYKVYQI
NKVEFI

Sequences:

>Translated_166_residues
MSDYVTTRFPITKGGYQKLESELEALKSERPKIIKSISDARELGDLSENAEYHAARERQGLVESKIMELESKKSRAEVIE
VSELSGDTVMFGATVTVSVYDEQSDTTSEVKYQIVGEYEADVSKNMISIKSPLVLALLGKREGDSVELKTPRGDYKVYQI
NKVEFI
>Mature_165_residues
SDYVTTRFPITKGGYQKLESELEALKSERPKIIKSISDARELGDLSENAEYHAARERQGLVESKIMELESKKSRAEVIEV
SELSGDTVMFGATVTVSVYDEQSDTTSEVKYQIVGEYEADVSKNMISIKSPLVLALLGKREGDSVELKTPRGDYKVYQIN
KVEFI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=160, Percent_Identity=48.125, Blast_Score=140, Evalue=6e-35,
Organism=Escherichia coli, GI87082261, Length=157, Percent_Identity=33.1210191082803, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18608; Mature: 18477

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYVTTRFPITKGGYQKLESELEALKSERPKIIKSISDARELGDLSENAEYHAARERQG
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
LVESKIMELESKKSRAEVIEVSELSGDTVMFGATVTVSVYDEQSDTTSEVKYQIVGEYEA
HHHHHHHHHHCCCCHHHEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCEEEEEEECCCC
DVSKNMISIKSPLVLALLGKREGDSVELKTPRGDYKVYQINKVEFI
CHHCCEEECCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEECC
>Mature Secondary Structure 
SDYVTTRFPITKGGYQKLESELEALKSERPKIIKSISDARELGDLSENAEYHAARERQG
CCCEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
LVESKIMELESKKSRAEVIEVSELSGDTVMFGATVTVSVYDEQSDTTSEVKYQIVGEYEA
HHHHHHHHHHCCCCHHHEEEEECCCCCEEEEEEEEEEEEECCCCCCCCCEEEEEEECCCC
DVSKNMISIKSPLVLALLGKREGDSVELKTPRGDYKVYQINKVEFI
CHHCCEEECCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA