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The map label for this gene is dus [H]

Identifier: 88607437

GI number: 88607437

Start: 602609

End: 603556

Strand: Direct

Name: dus [H]

Synonym: APH_0575

Alternate gene names: 88607437

Gene position: 602609-603556 (Clockwise)

Preceding gene: 88607646

Following gene: 88607510

Centisome position: 40.96

GC content: 42.51

Gene sequence:

>948_bases
ATGAGGCAATTCATCTCAGAAAACCCAGTTATACTTGCCCCTATGTCCGGCGTTACCGACTTGCCTTTTCGAACAACAGT
ACGAAAGCTGGGAGCTGGGCTTTTGATATCTGAAATGGTGGCCAGTAGGGCAATGATCTTAAAAACTCGGCAAAGTATGC
AAAAATCGGAAGTGTGTCCTAATACTTCTGTGCAATTGGCTGGTTGTACTCCTGAAATTATGGCAGAAGCGGCTAAGATT
AATGAAGATCTAGGTGCGAAATCTATAGATCTAAATTTTGGATGTCCTGCCAAAAAAGTTGTGGATGGCTATGCGGGCTC
TGCATTAATGAAGGATGAAGTTATCGCGACGAAAATAATGGAAAGCGTGGTCAAGGCGGTAAAAGTTCCTGTCACAGTAA
AAATGCGCATGGGCTGGGATACTACAAATCTGAATGCACCTACTTTGGCCAAAGTTGCTGAAGATGTAGGAATTCAGATG
ATTACCATTCACGGCAGAACGCGTTCACAGATGTTTAGTGGCTCTGCAGACTGGAAGTTCGTCAAGAGAGTAAAGGAGTG
TGTAAAAATACCAGTAATAGTAAACGGGGATATTAGAACCTTGGATGATATCGTAGTGGCGCTCAACGAATCCGGGGCAG
ATGGAGTGATGATTGGCAGAGGAGCCTACGGTAAACCATGGCTAATAAAGCAAGCACTAAGCTTTATGAAAAACTCGGAG
ATATTACCAGAGCCCACAAAAAGTGAAAAGTTGGCAATTATATTGGATCACTACGATTATGCACTAGACTACTATGGAAA
AGATCTAGGAGTAAAAATCTTGAGGAAGCACCTGAGTTGGTATAGCAGTTCCTATTCTGGTGCATCTTATTTTAGAGCGC
AAGTAAACACTATGAGTGACCCTGTAGAGGTAAGGGAAAAGCTAACAGAGTTTTTTCAAACATCGTGA

Upstream 100 bases:

>100_bases
TTATCACTAAATAAACTATCTATGGTAATAATTTCTATATTGCGACTGTCATGGTTGCGCTTTATTATCGGCCTATATGC
GATTAATGAACAATATTTAA

Downstream 100 bases:

>100_bases
TAAACGCGTAAAAGGTAGTAAATTATTGGACATTTTGCAGAATTTATGTTAGTGGGAGTGCCTACCAGTTGTGTTTTATA
TCGGTATCACTTGCGGTTTT

Product: putative tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRQFISENPVILAPMSGVTDLPFRTTVRKLGAGLLISEMVASRAMILKTRQSMQKSEVCPNTSVQLAGCTPEIMAEAAKI
NEDLGAKSIDLNFGCPAKKVVDGYAGSALMKDEVIATKIMESVVKAVKVPVTVKMRMGWDTTNLNAPTLAKVAEDVGIQM
ITIHGRTRSQMFSGSADWKFVKRVKECVKIPVIVNGDIRTLDDIVVALNESGADGVMIGRGAYGKPWLIKQALSFMKNSE
ILPEPTKSEKLAIILDHYDYALDYYGKDLGVKILRKHLSWYSSSYSGASYFRAQVNTMSDPVEVREKLTEFFQTS

Sequences:

>Translated_315_residues
MRQFISENPVILAPMSGVTDLPFRTTVRKLGAGLLISEMVASRAMILKTRQSMQKSEVCPNTSVQLAGCTPEIMAEAAKI
NEDLGAKSIDLNFGCPAKKVVDGYAGSALMKDEVIATKIMESVVKAVKVPVTVKMRMGWDTTNLNAPTLAKVAEDVGIQM
ITIHGRTRSQMFSGSADWKFVKRVKECVKIPVIVNGDIRTLDDIVVALNESGADGVMIGRGAYGKPWLIKQALSFMKNSE
ILPEPTKSEKLAIILDHYDYALDYYGKDLGVKILRKHLSWYSSSYSGASYFRAQVNTMSDPVEVREKLTEFFQTS
>Mature_315_residues
MRQFISENPVILAPMSGVTDLPFRTTVRKLGAGLLISEMVASRAMILKTRQSMQKSEVCPNTSVQLAGCTPEIMAEAAKI
NEDLGAKSIDLNFGCPAKKVVDGYAGSALMKDEVIATKIMESVVKAVKVPVTVKMRMGWDTTNLNAPTLAKVAEDVGIQM
ITIHGRTRSQMFSGSADWKFVKRVKECVKIPVIVNGDIRTLDDIVVALNESGADGVMIGRGAYGKPWLIKQALSFMKNSE
ILPEPTKSEKLAIILDHYDYALDYYGKDLGVKILRKHLSWYSSSYSGASYFRAQVNTMSDPVEVREKLTEFFQTS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=284, Percent_Identity=31.3380281690141, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI239788462, Length=284, Percent_Identity=31.3380281690141, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=31.2775330396476, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=32.1428571428571, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI8923374, Length=243, Percent_Identity=28.8065843621399, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1789660, Length=315, Percent_Identity=41.2698412698413, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1788462, Length=231, Percent_Identity=34.1991341991342, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693211, Length=233, Percent_Identity=30.0429184549356, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=281, Percent_Identity=32.0284697508897, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17507177, Length=241, Percent_Identity=27.8008298755187, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=29.0178571428571, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=307, Percent_Identity=27.6872964169381, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323560, Length=227, Percent_Identity=33.0396475770925, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323437, Length=242, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=32, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=32, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24585320, Length=294, Percent_Identity=30.952380952381, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19921524, Length=312, Percent_Identity=25.9615384615385, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45549423, Length=280, Percent_Identity=26.0714285714286, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34699; Mature: 34699

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQFISENPVILAPMSGVTDLPFRTTVRKLGAGLLISEMVASRAMILKTRQSMQKSEVCP
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NTSVQLAGCTPEIMAEAAKINEDLGAKSIDLNFGCPAKKVVDGYAGSALMKDEVIATKIM
CCCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHH
ESVVKAVKVPVTVKMRMGWDTTNLNAPTLAKVAEDVGIQMITIHGRTRSQMFSGSADWKF
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHCCCCCHHH
VKRVKECVKIPVIVNGDIRTLDDIVVALNESGADGVMIGRGAYGKPWLIKQALSFMKNSE
HHHHHHHHCCCEEECCCCHHHHHHHEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHCCC
ILPEPTKSEKLAIILDHYDYALDYYGKDLGVKILRKHLSWYSSSYSGASYFRAQVNTMSD
CCCCCCCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
PVEVREKLTEFFQTS
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MRQFISENPVILAPMSGVTDLPFRTTVRKLGAGLLISEMVASRAMILKTRQSMQKSEVCP
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NTSVQLAGCTPEIMAEAAKINEDLGAKSIDLNFGCPAKKVVDGYAGSALMKDEVIATKIM
CCCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHH
ESVVKAVKVPVTVKMRMGWDTTNLNAPTLAKVAEDVGIQMITIHGRTRSQMFSGSADWKF
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHCCCCCHHH
VKRVKECVKIPVIVNGDIRTLDDIVVALNESGADGVMIGRGAYGKPWLIKQALSFMKNSE
HHHHHHHHCCCEEECCCCHHHHHHHEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHCCC
ILPEPTKSEKLAIILDHYDYALDYYGKDLGVKILRKHLSWYSSSYSGASYFRAQVNTMSD
CCCCCCCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
PVEVREKLTEFFQTS
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA