Click here to switch to the map view.

The map label for this gene is lepA

Identifier: 88607108

GI number: 88607108

Start: 811008

End: 812804

Strand: Reverse

Name: lepA

Synonym: APH_0765

Alternate gene names: 88607108

Gene position: 812804-811008 (Counterclockwise)

Preceding gene: 88607046

Following gene: 88607650

Centisome position: 55.24

GC content: 41.46

Gene sequence:

>1797_bases
GTGAATAGGAGTTCGATACGGAATTTTGCGATAATAGCGCATATAGATCATGGAAAATCAACTTTAGCTGATAGGCTGAT
AGAGTCTTGTGATGCTTTGGCTGAGCGGGATATGAAGGAACAGGTTCTTGATTCCATGGATATCGAGCGTGAGCGCGGTA
TTACGATAAAAGCTCAAACCGTAAGGTTAAAGTATACATCTAAGGCGGGAGAAGTTTACTACTTAAACCTTGTTGATACT
CCCGGGCACGTTGATTTTTCATACGAAGTTAGCAGAAGCTTAGCTGCGTGTGAGGGTTCATTGCTTGTTATAGATAGTAG
TCAGGGTGTTGAAGCTCAGACATTGGCAAATGTCTATAAAGCGATTGAAAATAATCATGAGATAATAACAGTACTGAATA
AAGTAGACCTTGTCTCTGCAGATCCTGAAAAAGTTAAGTCACAAGTTGAAACGATAATAGGGCTAGATGCAAGTGATGCT
CTTTTGGTATCAGCTAAGACTGGTATAGGAATAAATGATGTTTTAGAGGCTATAATAGAGCGATTACCTGCGCCTGAAGG
TGATGAGGAAGCTCCTTTAAAAGCGGTATTGGTGGATAGTTGGTATGACCCATATCTGGGCATTGTCATTTTAGTGCGTA
TTAAAGACGGTGTATTGAAAAAGGGAATGAAGATATGCATGATGTCCACGGGAGCGGTATATACCGTAGATAATGTGGGT
GTATTTACTCCGCACAAGAAAATGGTTGATGCTTTGTCTGTAGGTGAGATTGGGTTTATCACTGCGGGCATAAAAGAGCT
TTTAGCTTGTAAAGTAGGGGATACTGTTACAGAAGATGCTAGAAGATGTGCTGAAGCTTTGCCGGGATTTAGGGCTACCT
GTCCGGTGGTCTTTTGTAGCCTTTTCCCTGTTGATGCTGGAAGTTTTGAACATTTAAGAGAGGCTTTGGGGAAGCTGCAA
TTAAATGACGCCAGCTTTACTTTTGATATAGAGAGTTCCACTGCTTTGGGATACGGTTTCCGCTGTGGTTTCCTAGGCAT
GCTTCATCTTGAAGTTGTCCAAGAGCGGCTAGAGCGGGAGTTTGATTTAGATCTTACTGCTACTGCTCCTAGTGTTGTAT
ATAAGGTGACTGATAAGCGTAATGTCACAAGGGACATTCATAATCCTAATGATTTACCAGAAGCACATGAGATTCTCAAG
GTGGAAGAGCCTTGGATAATGGCTACAATAATGGTTCCAGATCAGTATCTAGGATCTATGCTGGCTCTGTGCAATGGTAA
ACGCGGAGAGCGTGTAGATCTCTCGTATACTGGTAACATGGCTCTGTTGCAGTATCGGTTACCTTTAGCAGAAATAGTTT
TTGATTTTTATGATAGGTTAAAATCTATCTCGAAAGGGTATGCAAGCTTTGATTGGCAGATGGATGGTTATATGCCTGCC
GAGGTATCAAAATTGACTTTTTTGATTAATTCTGAGTTGGTAGATGCATTGGCGTGTATCATACATAAGTCAAAGATAGA
ATCAAGAGGGCGAGAAATTTGTGAACGCTTGAAGGATCTTATTCCTAGGCAGCAATATAAGATTGCAATACAGGCTGCAG
TTGGTTCAAAGATAGTAGCGCGTGAGACTATATCTCCATATCGTAAGGACGTGACAGCAAAGGTGTATGGGCGCGATGTT
ACGAGAAAGATGAAACTGTTGGAAAAGCAGAAGAAGGGGAAGAAGCGTCTGAGGTCTATTGGAAATATTACTGTTCCGCA
GAGTGCTTTTATTCAAGCATTGCAGGTGAAAGATTAG

Upstream 100 bases:

>100_bases
TGTCTATATGCCTTCTTGAGAAACTGATATAAATTATTACATTGAAAGGGAGATGATAGTAACTTAAACTTTGGCTTTAG
ATGTTGCTTGTGGTCCTAGA

Downstream 100 bases:

>100_bases
TTTCGGGTTATCTGTAAGTTTCAGAAGTGGATAGTAGGTATTCAGTCATTAATTTCAGGATTTTATCTTACTATATGAAA
CGCTGGGTTTGGAATATATC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MNRSSIRNFAIIAHIDHGKSTLADRLIESCDALAERDMKEQVLDSMDIERERGITIKAQTVRLKYTSKAGEVYYLNLVDT
PGHVDFSYEVSRSLAACEGSLLVIDSSQGVEAQTLANVYKAIENNHEIITVLNKVDLVSADPEKVKSQVETIIGLDASDA
LLVSAKTGIGINDVLEAIIERLPAPEGDEEAPLKAVLVDSWYDPYLGIVILVRIKDGVLKKGMKICMMSTGAVYTVDNVG
VFTPHKKMVDALSVGEIGFITAGIKELLACKVGDTVTEDARRCAEALPGFRATCPVVFCSLFPVDAGSFEHLREALGKLQ
LNDASFTFDIESSTALGYGFRCGFLGMLHLEVVQERLEREFDLDLTATAPSVVYKVTDKRNVTRDIHNPNDLPEAHEILK
VEEPWIMATIMVPDQYLGSMLALCNGKRGERVDLSYTGNMALLQYRLPLAEIVFDFYDRLKSISKGYASFDWQMDGYMPA
EVSKLTFLINSELVDALACIIHKSKIESRGREICERLKDLIPRQQYKIAIQAAVGSKIVARETISPYRKDVTAKVYGRDV
TRKMKLLEKQKKGKKRLRSIGNITVPQSAFIQALQVKD

Sequences:

>Translated_598_residues
MNRSSIRNFAIIAHIDHGKSTLADRLIESCDALAERDMKEQVLDSMDIERERGITIKAQTVRLKYTSKAGEVYYLNLVDT
PGHVDFSYEVSRSLAACEGSLLVIDSSQGVEAQTLANVYKAIENNHEIITVLNKVDLVSADPEKVKSQVETIIGLDASDA
LLVSAKTGIGINDVLEAIIERLPAPEGDEEAPLKAVLVDSWYDPYLGIVILVRIKDGVLKKGMKICMMSTGAVYTVDNVG
VFTPHKKMVDALSVGEIGFITAGIKELLACKVGDTVTEDARRCAEALPGFRATCPVVFCSLFPVDAGSFEHLREALGKLQ
LNDASFTFDIESSTALGYGFRCGFLGMLHLEVVQERLEREFDLDLTATAPSVVYKVTDKRNVTRDIHNPNDLPEAHEILK
VEEPWIMATIMVPDQYLGSMLALCNGKRGERVDLSYTGNMALLQYRLPLAEIVFDFYDRLKSISKGYASFDWQMDGYMPA
EVSKLTFLINSELVDALACIIHKSKIESRGREICERLKDLIPRQQYKIAIQAAVGSKIVARETISPYRKDVTAKVYGRDV
TRKMKLLEKQKKGKKRLRSIGNITVPQSAFIQALQVKD
>Mature_598_residues
MNRSSIRNFAIIAHIDHGKSTLADRLIESCDALAERDMKEQVLDSMDIERERGITIKAQTVRLKYTSKAGEVYYLNLVDT
PGHVDFSYEVSRSLAACEGSLLVIDSSQGVEAQTLANVYKAIENNHEIITVLNKVDLVSADPEKVKSQVETIIGLDASDA
LLVSAKTGIGINDVLEAIIERLPAPEGDEEAPLKAVLVDSWYDPYLGIVILVRIKDGVLKKGMKICMMSTGAVYTVDNVG
VFTPHKKMVDALSVGEIGFITAGIKELLACKVGDTVTEDARRCAEALPGFRATCPVVFCSLFPVDAGSFEHLREALGKLQ
LNDASFTFDIESSTALGYGFRCGFLGMLHLEVVQERLEREFDLDLTATAPSVVYKVTDKRNVTRDIHNPNDLPEAHEILK
VEEPWIMATIMVPDQYLGSMLALCNGKRGERVDLSYTGNMALLQYRLPLAEIVFDFYDRLKSISKGYASFDWQMDGYMPA
EVSKLTFLINSELVDALACIIHKSKIESRGREICERLKDLIPRQQYKIAIQAAVGSKIVARETISPYRKDVTAKVYGRDV
TRKMKLLEKQKKGKKRLRSIGNITVPQSAFIQALQVKD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=46.5116279069767, Blast_Score=583, Evalue=1e-166,
Organism=Homo sapiens, GI94966754, Length=186, Percent_Identity=37.6344086021505, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=38.3561643835616, Blast_Score=100, Evalue=7e-21,
Organism=Homo sapiens, GI18390331, Length=159, Percent_Identity=33.9622641509434, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI25306283, Length=180, Percent_Identity=40, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI19923640, Length=180, Percent_Identity=40, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI25306287, Length=180, Percent_Identity=40, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=41.5929203539823, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=41.5929203539823, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=41.5929203539823, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI217272892, Length=138, Percent_Identity=35.5072463768116, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI217272894, Length=138, Percent_Identity=35.5072463768116, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI53729339, Length=222, Percent_Identity=30.6306306306306, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI53729337, Length=222, Percent_Identity=30.6306306306306, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI94966752, Length=147, Percent_Identity=31.9727891156463, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI34147630, Length=278, Percent_Identity=25.1798561151079, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788922, Length=593, Percent_Identity=54.8060708263069, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=516, Percent_Identity=30.0387596899225, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789738, Length=160, Percent_Identity=35.625, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1790835, Length=152, Percent_Identity=32.8947368421053, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789559, Length=242, Percent_Identity=27.6859504132231, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=39.5081967213115, Blast_Score=460, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17556745, Length=464, Percent_Identity=26.7241379310345, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71988819, Length=132, Percent_Identity=39.3939393939394, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=132, Percent_Identity=39.3939393939394, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=145, Percent_Identity=36.551724137931, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=221, Percent_Identity=28.5067873303167, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.8345864661654, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32566303, Length=280, Percent_Identity=23.5714285714286, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=593, Percent_Identity=43.6762225969646, Blast_Score=517, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6323098, Length=202, Percent_Identity=32.1782178217822, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=36.986301369863, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=36.986301369863, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322359, Length=140, Percent_Identity=35, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=172, Percent_Identity=33.7209302325581, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=281, Percent_Identity=26.3345195729537, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=44.2404006677796, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI28574573, Length=233, Percent_Identity=36.0515021459227, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24582462, Length=159, Percent_Identity=35.2201257861635, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585711, Length=150, Percent_Identity=35.3333333333333, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585713, Length=150, Percent_Identity=35.3333333333333, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585709, Length=150, Percent_Identity=35.3333333333333, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357743, Length=180, Percent_Identity=33.8888888888889, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI221458488, Length=192, Percent_Identity=36.9791666666667, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281363316, Length=296, Percent_Identity=25.3378378378378, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI17864358, Length=296, Percent_Identity=25.3378378378378, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19921738, Length=294, Percent_Identity=25.8503401360544, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_ANAPZ (Q2GJV7)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505344.1
- ProteinModelPortal:   Q2GJV7
- SMR:   Q2GJV7
- STRING:   Q2GJV7
- GeneID:   3930016
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0765
- NMPDR:   fig|212042.5.peg.740
- TIGR:   APH_0765
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- PhylomeDB:   Q2GJV7
- ProtClustDB:   PRK05433
- BioCyc:   APHA212042:APH_0765-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66383; Mature: 66383

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSSIRNFAIIAHIDHGKSTLADRLIESCDALAERDMKEQVLDSMDIERERGITIKAQT
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEE
VRLKYTSKAGEVYYLNLVDTPGHVDFSYEVSRSLAACEGSLLVIDSSQGVEAQTLANVYK
EEEEEECCCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
AIENNHEIITVLNKVDLVSADPEKVKSQVETIIGLDASDALLVSAKTGIGINDVLEAIIE
HHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
RLPAPEGDEEAPLKAVLVDSWYDPYLGIVILVRIKDGVLKKGMKICMMSTGAVYTVDNVG
HCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCHHHHCCCEEEEEECCCEEEECCCC
VFTPHKKMVDALSVGEIGFITAGIKELLACKVGDTVTEDARRCAEALPGFRATCPVVFCS
EECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LFPVDAGSFEHLREALGKLQLNDASFTFDIESSTALGYGFRCGFLGMLHLEVVQERLERE
HCCCCCCCHHHHHHHHCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FDLDLTATAPSVVYKVTDKRNVTRDIHNPNDLPEAHEILKVEEPWIMATIMVPDQYLGSM
CCCEEEECCCEEEEEECCCCCCHHCCCCCCCCCHHHHHEEECCCEEEEEEECCHHHHHHH
LALCNGKRGERVDLSYTGNMALLQYRLPLAEIVFDFYDRLKSISKGYASFDWQMDGYMPA
HHHHCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECEEECCCCCC
EVSKLTFLINSELVDALACIIHKSKIESRGREICERLKDLIPRQQYKIAIQAAVGSKIVA
HHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEHHCCHHHH
RETISPYRKDVTAKVYGRDVTRKMKLLEKQKKGKKRLRSIGNITVPQSAFIQALQVKD
HHHHCHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNRSSIRNFAIIAHIDHGKSTLADRLIESCDALAERDMKEQVLDSMDIERERGITIKAQT
CCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEE
VRLKYTSKAGEVYYLNLVDTPGHVDFSYEVSRSLAACEGSLLVIDSSQGVEAQTLANVYK
EEEEEECCCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHH
AIENNHEIITVLNKVDLVSADPEKVKSQVETIIGLDASDALLVSAKTGIGINDVLEAIIE
HHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
RLPAPEGDEEAPLKAVLVDSWYDPYLGIVILVRIKDGVLKKGMKICMMSTGAVYTVDNVG
HCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCHHHHCCCEEEEEECCCEEEECCCC
VFTPHKKMVDALSVGEIGFITAGIKELLACKVGDTVTEDARRCAEALPGFRATCPVVFCS
EECCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
LFPVDAGSFEHLREALGKLQLNDASFTFDIESSTALGYGFRCGFLGMLHLEVVQERLERE
HCCCCCCCHHHHHHHHCCEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FDLDLTATAPSVVYKVTDKRNVTRDIHNPNDLPEAHEILKVEEPWIMATIMVPDQYLGSM
CCCEEEECCCEEEEEECCCCCCHHCCCCCCCCCHHHHHEEECCCEEEEEEECCHHHHHHH
LALCNGKRGERVDLSYTGNMALLQYRLPLAEIVFDFYDRLKSISKGYASFDWQMDGYMPA
HHHHCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEECEEECCCCCC
EVSKLTFLINSELVDALACIIHKSKIESRGREICERLKDLIPRQQYKIAIQAAVGSKIVA
HHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEHHCCHHHH
RETISPYRKDVTAKVYGRDVTRKMKLLEKQKKGKKRLRSIGNITVPQSAFIQALQVKD
HHHHCHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA