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The map label for this gene is pheS [H]

Identifier: 88607026

GI number: 88607026

Start: 67898

End: 68926

Strand: Reverse

Name: pheS [H]

Synonym: APH_0059

Alternate gene names: 88607026

Gene position: 68926-67898 (Counterclockwise)

Preceding gene: 88606750

Following gene: 88606715

Centisome position: 4.68

GC content: 44.31

Gene sequence:

>1029_bases
TTGCTCAGTCAAATTCATGATCTAGGAAAGGAAGCGTTTGCTTCTGTTGCTGATTGTAGTTCTCGAGAAGAGCTTGATGA
GGTGCGGGTAAAGTATCTTGGAAAGTCAGGCCTTCTTACTGCCCTTCTTAAGCAGATTTCCAAGATTGAAGATCTTGAAG
AGCGGAAAAAAGTCGGAAGTGAGACTAATGTCTTGTGCTCAGAGTTCAAGGCGTTGCTGCATCAGAGAGAGGCAGAATTC
GAGCGAGAGCTGCTGTATGCTAGGTTGCGTAAAGATAGCATAGATATTACTTTGCCGTCTAGGCCGCGTGTATATGGGAG
GTTGCATCCTGTAACAAAGGTAGTCAAGGATATCGAAGATATTTTCATCGCGCTGGGGTTTGCTACTGTTCGTGGTCCAG
ATCTTGAAGATGAGTTTCACGTCTTTGACGCTTTAAACACACCTGTACATCATCCAGCCAGAGAAAAGAACGATACTTTC
TATATGGCTCGTGATAATCAAGGCAAGCGTGTTGTCTTAAGGACGCACACGTCGTCGGTGCAGATTCGGACAATGGAAAC
TAGTTCTGAAATGCCTATCAAGATAATATCTTCGGGTAAGGTGTATAGAAATGACTGGGATGCTACGCACAGCCCGATGT
TTCACCAGGTCGAGGGGTTATACGTTGGCAGCGATGTTACGATGGGGCACCTAAAGTATTGTATCAATCACTTTCTTGAG
AAGTTTTTTGGACGCAAAATCAAAATGAGAATGCGCGCAAGCTTTTTTCCTTTTACGGAGCCATCTGCCGAGATAGACAT
AAGGGATAGTCGCGGTCAGTGGGTTGAGGTGCTAGGCTGCGGTATGGTGCATAACAGGGTATTGGAAAACGTAAATATAG
ATAGCTCTAAATACAGTGGTTTTGCTTTCGGAATGGGTGTTGAGAGAATGGCAATGCTTAAGTACGACATTGGAGACCTC
AGGGATTTATATTGTAGTCGCATTGACTGGTTAGGGCATTACGGCTTTTGTTTTACAGATCTTGTATAG

Upstream 100 bases:

>100_bases
TTAAACAGAAAGATGTTGGCGGAGATGGCTGTCAGAGACAAGGGAGCTTTTGAAAAGATTGCTCATGCTGCAGCACAAGC
TTGTGGGCGAACCTGTGTCG

Downstream 100 bases:

>100_bases
TATTTCTTTGTATTTTCTTCAAAATTTTGAGATAGCTTAAACTAGAAGAATATTCTGCAAAGTTAGTCTTGCATGCAAAG
TCCTGCTTTTCACACCATTG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLSQIHDLGKEAFASVADCSSREELDEVRVKYLGKSGLLTALLKQISKIEDLEERKKVGSETNVLCSEFKALLHQREAEF
ERELLYARLRKDSIDITLPSRPRVYGRLHPVTKVVKDIEDIFIALGFATVRGPDLEDEFHVFDALNTPVHHPAREKNDTF
YMARDNQGKRVVLRTHTSSVQIRTMETSSEMPIKIISSGKVYRNDWDATHSPMFHQVEGLYVGSDVTMGHLKYCINHFLE
KFFGRKIKMRMRASFFPFTEPSAEIDIRDSRGQWVEVLGCGMVHNRVLENVNIDSSKYSGFAFGMGVERMAMLKYDIGDL
RDLYCSRIDWLGHYGFCFTDLV

Sequences:

>Translated_342_residues
MLSQIHDLGKEAFASVADCSSREELDEVRVKYLGKSGLLTALLKQISKIEDLEERKKVGSETNVLCSEFKALLHQREAEF
ERELLYARLRKDSIDITLPSRPRVYGRLHPVTKVVKDIEDIFIALGFATVRGPDLEDEFHVFDALNTPVHHPAREKNDTF
YMARDNQGKRVVLRTHTSSVQIRTMETSSEMPIKIISSGKVYRNDWDATHSPMFHQVEGLYVGSDVTMGHLKYCINHFLE
KFFGRKIKMRMRASFFPFTEPSAEIDIRDSRGQWVEVLGCGMVHNRVLENVNIDSSKYSGFAFGMGVERMAMLKYDIGDL
RDLYCSRIDWLGHYGFCFTDLV
>Mature_342_residues
MLSQIHDLGKEAFASVADCSSREELDEVRVKYLGKSGLLTALLKQISKIEDLEERKKVGSETNVLCSEFKALLHQREAEF
ERELLYARLRKDSIDITLPSRPRVYGRLHPVTKVVKDIEDIFIALGFATVRGPDLEDEFHVFDALNTPVHHPAREKNDTF
YMARDNQGKRVVLRTHTSSVQIRTMETSSEMPIKIISSGKVYRNDWDATHSPMFHQVEGLYVGSDVTMGHLKYCINHFLE
KFFGRKIKMRMRASFFPFTEPSAEIDIRDSRGQWVEVLGCGMVHNRVLENVNIDSSKYSGFAFGMGVERMAMLKYDIGDL
RDLYCSRIDWLGHYGFCFTDLV

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=266, Percent_Identity=29.3233082706767, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI5729820, Length=236, Percent_Identity=30.0847457627119, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1788007, Length=333, Percent_Identity=46.2462462462462, Blast_Score=312, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI32563657, Length=314, Percent_Identity=28.9808917197452, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=314, Percent_Identity=28.9808917197452, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=245, Percent_Identity=29.7959183673469, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=382, Percent_Identity=26.4397905759162, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=262, Percent_Identity=27.4809160305344, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18858079, Length=365, Percent_Identity=27.9452054794521, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI17137424, Length=243, Percent_Identity=27.5720164609054, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39304; Mature: 39304

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSQIHDLGKEAFASVADCSSREELDEVRVKYLGKSGLLTALLKQISKIEDLEERKKVGS
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
ETNVLCSEFKALLHQREAEFERELLYARLRKDSIDITLPSRPRVYGRLHPVTKVVKDIED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
IFIALGFATVRGPDLEDEFHVFDALNTPVHHPAREKNDTFYMARDNQGKRVVLRTHTSSV
HHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCE
QIRTMETSSEMPIKIISSGKVYRNDWDATHSPMFHQVEGLYVGSDVTMGHLKYCINHFLE
EEEEECCCCCCCEEEECCCCEEECCCCCCCCCHHHHHCCEEECCCCHHHHHHHHHHHHHH
KFFGRKIKMRMRASFFPFTEPSAEIDIRDSRGQWVEVLGCGMVHNRVLENVNIDSSKYSG
HHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCC
FAFGMGVERMAMLKYDIGDLRDLYCSRIDWLGHYGFCFTDLV
EEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MLSQIHDLGKEAFASVADCSSREELDEVRVKYLGKSGLLTALLKQISKIEDLEERKKVGS
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
ETNVLCSEFKALLHQREAEFERELLYARLRKDSIDITLPSRPRVYGRLHPVTKVVKDIED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
IFIALGFATVRGPDLEDEFHVFDALNTPVHHPAREKNDTFYMARDNQGKRVVLRTHTSSV
HHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCE
QIRTMETSSEMPIKIISSGKVYRNDWDATHSPMFHQVEGLYVGSDVTMGHLKYCINHFLE
EEEEECCCCCCCEEEECCCCEEECCCCCCCCCHHHHHCCEEECCCCHHHHHHHHHHHHHH
KFFGRKIKMRMRASFFPFTEPSAEIDIRDSRGQWVEVLGCGMVHNRVLENVNIDSSKYSG
HHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCC
FAFGMGVERMAMLKYDIGDLRDLYCSRIDWLGHYGFCFTDLV
EEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA