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The map label for this gene is lpdA-1 [H]

Identifier: 88606942

GI number: 88606942

Start: 72028

End: 73443

Strand: Reverse

Name: lpdA-1 [H]

Synonym: APH_0065

Alternate gene names: 88606942

Gene position: 73443-72028 (Counterclockwise)

Preceding gene: 88607533

Following gene: 88607195

Centisome position: 4.99

GC content: 45.06

Gene sequence:

>1416_bases
ATGGACAAGTACGAAGTAGTAATAATCGGGAGTGGACCTGGAGGATATATAGCTGCTATTCGCGCAGCGCAGCTCGGATA
TAACGTGGCTATAGTGGAGCGTGAAGACAACCTGGGAGGCGTGTGCCTAAATTGGGGTTGCATTCCCACAAAGGCTTTGC
TCAAATCTGCCCAACTGTACAAAAAGATCCTGTCTGCTAGTTCCTTTGGTATTAAAATTACGGGAGATGTAGAGGTAGAC
ATTCAGTCAATCGTGGCACACTCTAGAGACGCTGTGGCTAAGCTGAGCTGCGGTGTATCTATGCTCATGAAAAAGAACGG
CGTTAAGGTGTATAAAGGATGCGCGCGTATTGCCGGTAAAGGAGAGATTCATGTTGATAATGACGGAGTAAAAAGTGCCC
TCTCGGCTAAACATATAATACTTGCCACCGGTGGAAGGCCAAGAATTGCTACCAATTTAGATACAAAATTGCTTTGGTCC
TCCAAAGATGCCATGCTCCCTGAAACGTTGCCTAAATCTCTCTTGATCATTGGTAGTGGCGCAATTGGTATTGAGTTTGC
CAGTTTCTACAGCACCATTGGTAGTAAAGTTACCATTGTAGAAATGCAAGATAGAATTCTGCCATTGGAAGATCGTGACA
TATCGTTGTCTATGCATGAGATATTGAAAAACCAGGGAGTAGATATATTTACTGCTTGCTCAGTTATGGATTTAGTCCAG
AGTGCTTCTTCCATTACAGCTCAGATCGTCAACAGTGGCACAAAAGATACTGTAACTTCTTCTTTTGAAAGGGTAATTTG
CGCCATAGGTATTTTGCCTAATTCCGGAAACCTAGGCTTGGAGGATACTAAAGTACAGCTCGATAAGGGGGGCTTTATCA
TCACAGACGGTATGTGCCAAACATCAGAGCCCGGAATATACGCCATCGGAGATGTAGCAGGGCCTCCATGTTTAGCTCAC
AAAGCTAGTCACGAAGCTGTGATTTGTGTTGAGGGTATTGCTAAAAAAGACGGTCGTATATCAACAGCACCCTCCACTCT
ACATAAAAATAATATACCCAGCTGCATTTATTCGATTCCTCAGATCGCTAGCGTGGGATTGACTGAAGATGCCGCTAAAG
CCCAAGGCCTAGAGATAAAAGTTGGTATCTCACGCGCTAGCTGCAACGGTAAAGCCATTGCCTCTGGGGAATCTGAGGGT
TTCGTAAAGGTTATTCTGTGCTCCAAGACTGGAGAACTACTCGGAGCTCACATGCTCGGAAGTGAAGTTACAGAGATGAT
CAACGGATACATAGTAGGGCGTCAGCTTGAGGCTACAGATCTCGATATAGCTCATACCATATTCCCTCACCCCACACTCT
CTGAAATGATGCACTCTGCCATACTCTCCGCTTGGAACGAACCTCTGGATAGTTAA

Upstream 100 bases:

>100_bases
ATGGTCCGGCTACTATTCGCGCCCCATGTCATTGTATTTATCCGTATCCACATGCAGTAAAACAAACCTGATTTACGTAT
AATTTCCTTTCCCTACTCAC

Downstream 100 bases:

>100_bases
CCATACCCAGTCTTTCCTAGGTGTAGACACCAAACGATGCTGCAGCCATCGTTACGCCTAATCGTACCCGTACAGATAAC
CGTTAGCTATTCATCATTTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MDKYEVVIIGSGPGGYIAAIRAAQLGYNVAIVEREDNLGGVCLNWGCIPTKALLKSAQLYKKILSASSFGIKITGDVEVD
IQSIVAHSRDAVAKLSCGVSMLMKKNGVKVYKGCARIAGKGEIHVDNDGVKSALSAKHIILATGGRPRIATNLDTKLLWS
SKDAMLPETLPKSLLIIGSGAIGIEFASFYSTIGSKVTIVEMQDRILPLEDRDISLSMHEILKNQGVDIFTACSVMDLVQ
SASSITAQIVNSGTKDTVTSSFERVICAIGILPNSGNLGLEDTKVQLDKGGFIITDGMCQTSEPGIYAIGDVAGPPCLAH
KASHEAVICVEGIAKKDGRISTAPSTLHKNNIPSCIYSIPQIASVGLTEDAAKAQGLEIKVGISRASCNGKAIASGESEG
FVKVILCSKTGELLGAHMLGSEVTEMINGYIVGRQLEATDLDIAHTIFPHPTLSEMMHSAILSAWNEPLDS

Sequences:

>Translated_471_residues
MDKYEVVIIGSGPGGYIAAIRAAQLGYNVAIVEREDNLGGVCLNWGCIPTKALLKSAQLYKKILSASSFGIKITGDVEVD
IQSIVAHSRDAVAKLSCGVSMLMKKNGVKVYKGCARIAGKGEIHVDNDGVKSALSAKHIILATGGRPRIATNLDTKLLWS
SKDAMLPETLPKSLLIIGSGAIGIEFASFYSTIGSKVTIVEMQDRILPLEDRDISLSMHEILKNQGVDIFTACSVMDLVQ
SASSITAQIVNSGTKDTVTSSFERVICAIGILPNSGNLGLEDTKVQLDKGGFIITDGMCQTSEPGIYAIGDVAGPPCLAH
KASHEAVICVEGIAKKDGRISTAPSTLHKNNIPSCIYSIPQIASVGLTEDAAKAQGLEIKVGISRASCNGKAIASGESEG
FVKVILCSKTGELLGAHMLGSEVTEMINGYIVGRQLEATDLDIAHTIFPHPTLSEMMHSAILSAWNEPLDS
>Mature_471_residues
MDKYEVVIIGSGPGGYIAAIRAAQLGYNVAIVEREDNLGGVCLNWGCIPTKALLKSAQLYKKILSASSFGIKITGDVEVD
IQSIVAHSRDAVAKLSCGVSMLMKKNGVKVYKGCARIAGKGEIHVDNDGVKSALSAKHIILATGGRPRIATNLDTKLLWS
SKDAMLPETLPKSLLIIGSGAIGIEFASFYSTIGSKVTIVEMQDRILPLEDRDISLSMHEILKNQGVDIFTACSVMDLVQ
SASSITAQIVNSGTKDTVTSSFERVICAIGILPNSGNLGLEDTKVQLDKGGFIITDGMCQTSEPGIYAIGDVAGPPCLAH
KASHEAVICVEGIAKKDGRISTAPSTLHKNNIPSCIYSIPQIASVGLTEDAAKAQGLEIKVGISRASCNGKAIASGESEG
FVKVILCSKTGELLGAHMLGSEVTEMINGYIVGRQLEATDLDIAHTIFPHPTLSEMMHSAILSAWNEPLDS

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=34.9680170575693, Blast_Score=263, Evalue=4e-70,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=29.5744680851064, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI22035672, Length=462, Percent_Identity=29.004329004329, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI33519430, Length=463, Percent_Identity=23.7580993520518, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519428, Length=463, Percent_Identity=23.7580993520518, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI33519426, Length=463, Percent_Identity=23.7580993520518, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148277065, Length=463, Percent_Identity=23.7580993520518, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI148277071, Length=464, Percent_Identity=23.7068965517241, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI291045266, Length=434, Percent_Identity=23.0414746543779, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI291045268, Length=283, Percent_Identity=24.0282685512367, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=33.4061135371179, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082354, Length=482, Percent_Identity=28.2157676348548, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=28.5407725321888, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789915, Length=445, Percent_Identity=26.7415730337079, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=34.6153846153846, Blast_Score=265, Evalue=5e-71,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=25.8947368421053, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=445, Percent_Identity=24.7191011235955, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=445, Percent_Identity=24.7191011235955, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=488, Percent_Identity=23.9754098360656, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=36.6108786610879, Blast_Score=282, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=28.4210526315789, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=26.6524520255864, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21358499, Length=472, Percent_Identity=34.1101694915254, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=22.5206611570248, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640549, Length=489, Percent_Identity=22.6993865030675, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640553, Length=489, Percent_Identity=22.6993865030675, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640551, Length=488, Percent_Identity=22.5409836065574, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49680; Mature: 49680

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKYEVVIIGSGPGGYIAAIRAAQLGYNVAIVEREDNLGGVCLNWGCIPTKALLKSAQLY
CCCEEEEEEECCCCCCEEEEEHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
KKILSASSFGIKITGDVEVDIQSIVAHSRDAVAKLSCGVSMLMKKNGVKVYKGCARIAGK
HHHHCCCCCCEEEECCEEEEHHHHHHCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCC
GEIHVDNDGVKSALSAKHIILATGGRPRIATNLDTKLLWSSKDAMLPETLPKSLLIIGSG
CEEEECCCCHHHHHCCCEEEEEECCCCCEECCCCCEEEECCCCCCCCCCCCCEEEEEECC
AIGIEFASFYSTIGSKVTIVEMQDRILPLEDRDISLSMHEILKNQGVDIFTACSVMDLVQ
CCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHH
SASSITAQIVNSGTKDTVTSSFERVICAIGILPNSGNLGLEDTKVQLDKGGFIITDGMCQ
HHHHHHHHHHCCCCCHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEECCCEEEECCCCC
TSEPGIYAIGDVAGPPCLAHKASHEAVICVEGIAKKDGRISTAPSTLHKNNIPSCIYSIP
CCCCCEEEECCCCCCCHHHCCCCCCEEEEEECCCCCCCCEECCCCHHHCCCCCHHHHHCH
QIASVGLTEDAAKAQGLEIKVGISRASCNGKAIASGESEGFVKVILCSKTGELLGAHMLG
HHHHCCCCCHHHHCCCCEEEEEEEEECCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHH
SEVTEMINGYIVGRQLEATDLDIAHTIFPHPTLSEMMHSAILSAWNEPLDS
HHHHHHHCCEEEECEECCCCCCEEHEECCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDKYEVVIIGSGPGGYIAAIRAAQLGYNVAIVEREDNLGGVCLNWGCIPTKALLKSAQLY
CCCEEEEEEECCCCCCEEEEEHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHH
KKILSASSFGIKITGDVEVDIQSIVAHSRDAVAKLSCGVSMLMKKNGVKVYKGCARIAGK
HHHHCCCCCCEEEECCEEEEHHHHHHCCHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCC
GEIHVDNDGVKSALSAKHIILATGGRPRIATNLDTKLLWSSKDAMLPETLPKSLLIIGSG
CEEEECCCCHHHHHCCCEEEEEECCCCCEECCCCCEEEECCCCCCCCCCCCCEEEEEECC
AIGIEFASFYSTIGSKVTIVEMQDRILPLEDRDISLSMHEILKNQGVDIFTACSVMDLVQ
CCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEHHHHHHHHHH
SASSITAQIVNSGTKDTVTSSFERVICAIGILPNSGNLGLEDTKVQLDKGGFIITDGMCQ
HHHHHHHHHHCCCCCHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEECCCEEEECCCCC
TSEPGIYAIGDVAGPPCLAHKASHEAVICVEGIAKKDGRISTAPSTLHKNNIPSCIYSIP
CCCCCEEEECCCCCCCHHHCCCCCCEEEEEECCCCCCCCEECCCCHHHCCCCCHHHHHCH
QIASVGLTEDAAKAQGLEIKVGISRASCNGKAIASGESEGFVKVILCSKTGELLGAHMLG
HHHHCCCCCHHHHCCCCEEEEEEEEECCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHH
SEVTEMINGYIVGRQLEATDLDIAHTIFPHPTLSEMMHSAILSAWNEPLDS
HHHHHHHCCEEEECEECCCCCCEEHEECCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]