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The map label for this gene is miaB

Identifier: 88606847

GI number: 88606847

Start: 935608

End: 936942

Strand: Reverse

Name: miaB

Synonym: APH_0880

Alternate gene names: 88606847

Gene position: 936942-935608 (Counterclockwise)

Preceding gene: 88607373

Following gene: 88607142

Centisome position: 63.68

GC content: 44.27

Gene sequence:

>1335_bases
ATGACAAGGGGTCTGTATATAGAGTCCTACGGGTGTCAGATGAATGTGTACGACGCTCTAATGATGGAAGATCTGCTAAG
GCCTGTGGGCTATGCTGCAGTTTCGCGTCCAGAAGATGCTGACATAATATTGATTAATACTTGCCACATAAGGGAAAAGG
CGTCTGAGAAGCTTTACTCTACCCTCGGGCGTATGCGCGTAATAAAAAAGGAAGAGTGTATTCTGATAGTTGCGGGTTGT
GTGGCACAGGCTGAGGGCGAAGCTGTGTTTGAACGTGCGCCGTACGTGAATGTGGTTGTTGGGCCGCAGGGGCTGCATAC
TCTGCCAGAACTCATAATGAAGGTAAAAAGGGATAAGAAGCAGATCAACATAGAGTTCCCAGTGGTCTCTAAGTTTGACG
CAATTTCAATAGATAGAAAGGCTAATGGAGGGGTTTCTGCATATGTCTCCATTCAGGAAGGTTGCGATAAATTTTGTACT
TTCTGTGTAGTACCTTATACAAGAGGGCCGGAGTATTCTCGTGATGTAGAAGCTATATTGGAAGAGGTAAAACAACTTAC
TGATAGAGGTACAAAGGAGATAGTCCTAATAGGGCAGAACGTTAATGCCTACCATGGAACGTACAAAGGTAATGAGTGGG
ATTTAGGGAAGTTAATTCAAAAGGTATCACTAATAGACGGAGTTGAGAGAATACGGTACACAACCTCGCACCCCAGGGAT
ATGCATCCTTCTTTATATGAGGCTCATAGAGACGAGAAGAAACTGGCACCGTTTGTGCATTTACCCGTGCAGTCTGGTTC
TGATGCGATATTACGAAAGATGAATCGCAAGCACACTGCTGAAGAGTATCTTCGAGTGGTTGAGCAATTGAAAGACTCGA
ATAAAAATATGGCTCTGTCTTCAGATTTTATAGTTGGTTTTCCTGGTGAGACTGAAAAAGACTTTGAAGAGACTATGAAG
CTGGTAGAAAGCGTTGGCTTTGCCTTGTCGTATAGCTTTAAGTATAGCCCAAGGCCTGGTACTCCTGGTGCTGAGTATTC
TAATCAGGTACCAGAGGAAGAGAAGAGCGCGCGTTTGGCTGCCTTGCAGGGCCTACTTACAAAACAGCAATTGCAGTTCA
ATAAGAGTATGGAGGGCAGGGTAATGGATGTCTTAGTAGGTGATCCTAGTAGCATGCGGAGCGATAGGATTTTTGGAAAA
AGTGAATATACTCAGTCTATACACATATCGGCTCCAAGCGGATCAGAAGACTGTTTTAATAGGATGGTTAGGGTTGAAAT
ACTGCATGGACGGCAGAATTCTCTGGAAGGGACTGTGTTGTCTAATGCAAACTAG

Upstream 100 bases:

>100_bases
GAATTACTAGGTTTCTGGCAAGTGGCACACTGGAACTTCGTGATTGTATTACTTGTTTATGTGGTATAGAATCGCGTTTT
ATTCTCCAAAAAAAGTTCTT

Downstream 100 bases:

>100_bases
CATTATTTTATTAGCGGACGTGTATTGTTTTGATCTAATCAGAGCTAAATCCATGAGTCGTACCACTACATTACTGTTAT
GACAGCATTGTTCTTTGGTT

Product: tRNA-i(6)A37 thiotransferase enzyme MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTRGLYIESYGCQMNVYDALMMEDLLRPVGYAAVSRPEDADIILINTCHIREKASEKLYSTLGRMRVIKKEECILIVAGC
VAQAEGEAVFERAPYVNVVVGPQGLHTLPELIMKVKRDKKQINIEFPVVSKFDAISIDRKANGGVSAYVSIQEGCDKFCT
FCVVPYTRGPEYSRDVEAILEEVKQLTDRGTKEIVLIGQNVNAYHGTYKGNEWDLGKLIQKVSLIDGVERIRYTTSHPRD
MHPSLYEAHRDEKKLAPFVHLPVQSGSDAILRKMNRKHTAEEYLRVVEQLKDSNKNMALSSDFIVGFPGETEKDFEETMK
LVESVGFALSYSFKYSPRPGTPGAEYSNQVPEEEKSARLAALQGLLTKQQLQFNKSMEGRVMDVLVGDPSSMRSDRIFGK
SEYTQSIHISAPSGSEDCFNRMVRVEILHGRQNSLEGTVLSNAN

Sequences:

>Translated_444_residues
MTRGLYIESYGCQMNVYDALMMEDLLRPVGYAAVSRPEDADIILINTCHIREKASEKLYSTLGRMRVIKKEECILIVAGC
VAQAEGEAVFERAPYVNVVVGPQGLHTLPELIMKVKRDKKQINIEFPVVSKFDAISIDRKANGGVSAYVSIQEGCDKFCT
FCVVPYTRGPEYSRDVEAILEEVKQLTDRGTKEIVLIGQNVNAYHGTYKGNEWDLGKLIQKVSLIDGVERIRYTTSHPRD
MHPSLYEAHRDEKKLAPFVHLPVQSGSDAILRKMNRKHTAEEYLRVVEQLKDSNKNMALSSDFIVGFPGETEKDFEETMK
LVESVGFALSYSFKYSPRPGTPGAEYSNQVPEEEKSARLAALQGLLTKQQLQFNKSMEGRVMDVLVGDPSSMRSDRIFGK
SEYTQSIHISAPSGSEDCFNRMVRVEILHGRQNSLEGTVLSNAN
>Mature_443_residues
TRGLYIESYGCQMNVYDALMMEDLLRPVGYAAVSRPEDADIILINTCHIREKASEKLYSTLGRMRVIKKEECILIVAGCV
AQAEGEAVFERAPYVNVVVGPQGLHTLPELIMKVKRDKKQINIEFPVVSKFDAISIDRKANGGVSAYVSIQEGCDKFCTF
CVVPYTRGPEYSRDVEAILEEVKQLTDRGTKEIVLIGQNVNAYHGTYKGNEWDLGKLIQKVSLIDGVERIRYTTSHPRDM
HPSLYEAHRDEKKLAPFVHLPVQSGSDAILRKMNRKHTAEEYLRVVEQLKDSNKNMALSSDFIVGFPGETEKDFEETMKL
VESVGFALSYSFKYSPRPGTPGAEYSNQVPEEEKSARLAALQGLLTKQQLQFNKSMEGRVMDVLVGDPSSMRSDRIFGKS
EYTQSIHISAPSGSEDCFNRMVRVEILHGRQNSLEGTVLSNAN

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=429, Percent_Identity=32.1678321678322, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI28872782, Length=429, Percent_Identity=32.1678321678322, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI93277076, Length=466, Percent_Identity=26.8240343347639, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=45.6179775280899, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787057, Length=378, Percent_Identity=30.1587301587302, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17553146, Length=406, Percent_Identity=33.0049261083744, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=329, Percent_Identity=27.0516717325228, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356207, Length=441, Percent_Identity=31.7460317460317, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI19922432, Length=471, Percent_Identity=26.3269639065817, Blast_Score=138, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ANAPZ (Q2GJJ5)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505456.1
- ProteinModelPortal:   Q2GJJ5
- SMR:   Q2GJJ5
- STRING:   Q2GJJ5
- GeneID:   3929963
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0880
- NMPDR:   fig|212042.5.peg.835
- TIGR:   APH_0880
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- PhylomeDB:   Q2GJJ5
- ProtClustDB:   CLSK747303
- BioCyc:   APHA212042:APH_0880-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49839; Mature: 49707

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRGLYIESYGCQMNVYDALMMEDLLRPVGYAAVSRPEDADIILINTCHIREKASEKLYS
CCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHH
TLGRMRVIKKEECILIVAGCVAQAEGEAVFERAPYVNVVVGPQGLHTLPELIMKVKRDKK
HHHHHHHCCCCCEEEEEECHHHCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCC
QINIEFPVVSKFDAISIDRKANGGVSAYVSIQEGCDKFCTFCVVPYTRGPEYSRDVEAIL
EEEEEECCCCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
EEVKQLTDRGTKEIVLIGQNVNAYHGTYKGNEWDLGKLIQKVSLIDGVERIRYTTSHPRD
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
MHPSLYEAHRDEKKLAPFVHLPVQSGSDAILRKMNRKHTAEEYLRVVEQLKDSNKNMALS
CCCHHHHHHCCHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEE
SDFIVGFPGETEKDFEETMKLVESVGFALSYSFKYSPRPGTPGAEYSNQVPEEEKSARLA
CCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
ALQGLLTKQQLQFNKSMEGRVMDVLVGDPSSMRSDRIFGKSEYTQSIHISAPSGSEDCFN
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCCCCCCCCCCCCEEEEECCCCCHHHHH
RMVRVEILHGRQNSLEGTVLSNAN
HHEEEEEEECCCCCCCCCEECCCC
>Mature Secondary Structure 
TRGLYIESYGCQMNVYDALMMEDLLRPVGYAAVSRPEDADIILINTCHIREKASEKLYS
CCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHH
TLGRMRVIKKEECILIVAGCVAQAEGEAVFERAPYVNVVVGPQGLHTLPELIMKVKRDKK
HHHHHHHCCCCCEEEEEECHHHCCCCCHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCC
QINIEFPVVSKFDAISIDRKANGGVSAYVSIQEGCDKFCTFCVVPYTRGPEYSRDVEAIL
EEEEEECCCCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHEEEEECCCCCCCCHHHHHHH
EEVKQLTDRGTKEIVLIGQNVNAYHGTYKGNEWDLGKLIQKVSLIDGVERIRYTTSHPRD
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
MHPSLYEAHRDEKKLAPFVHLPVQSGSDAILRKMNRKHTAEEYLRVVEQLKDSNKNMALS
CCCHHHHHHCCHHHCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEE
SDFIVGFPGETEKDFEETMKLVESVGFALSYSFKYSPRPGTPGAEYSNQVPEEEKSARLA
CCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
ALQGLLTKQQLQFNKSMEGRVMDVLVGDPSSMRSDRIFGKSEYTQSIHISAPSGSEDCFN
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHCCCCCCCCCCCCCEEEEECCCCCHHHHH
RMVRVEILHGRQNSLEGTVLSNAN
HHEEEEEEECCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA