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The map label for this gene is thiC [H]

Identifier: 88606691

GI number: 88606691

Start: 615023

End: 615529

Strand: Direct

Name: thiC [H]

Synonym: APH_0586

Alternate gene names: 88606691

Gene position: 615023-615529 (Clockwise)

Preceding gene: 88607558

Following gene: 88607711

Centisome position: 41.8

GC content: 40.63

Gene sequence:

>507_bases
ATGCACATGAATATTTCTGAAATTTACCCATCATCTAATAAAATATACATTAATTGCACTCTATATCCGGATGTTAAGGT
CGGAATGCGTCAAATAAATATTAGAAATTCTTCGCAGCGTTTCCTTCTCTACGATACTGCTGGTCCTTATACTGATTGCG
ATATTAAGGTTAATCTGACTGAAGGAATATCTAGCATAAGGCGAGATTGGATAGCTCGCAGGAATGATACCTATCCTATA
CGTAAGGCTAAGACTAATTCTGATGCTCGAGGTGTAAAGGCTCTTTCTGGAGATGATAGAGTAATATTGAAAGGAATAGC
TGGAGGTGCTCCAGTGACTCAACTCTTTTATGCTAAAAAAGGTATCATAACACCCGAGATGGAGTATGTAGCCGTGCGGG
AGAACGCTTTGCTTGAACAGGCTGAAGCTATCCCTGGTCTGTTATTGCCTACTAAGACACGTAAAATAACACCTGAGTTT
GTGCGTGAAGAGGTGGCTTGCGTGTGA

Upstream 100 bases:

>100_bases
ACATCTTCTGACATAATATGTTTATAAAGAAGAGATTTTTATTTAAGATTGTATAATATATTCCATAAATGTATGCTAAC
CGGGAATATTTTTCATCCTA

Downstream 100 bases:

>100_bases
TATATGTGCAAAAACACATGTTTACTATGTGCATTTATATCTCAGTCTAGCCATGCTTAGTAGAGGAAAAATTATGCTAA
GAGGAAGATATATAGAAGAG

Product: thiamine biosynthesis protein ThiC, truncation

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLTEGISSIRRDWIARRNDTYPI
RKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKKGIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEF
VREEVACV

Sequences:

>Translated_168_residues
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLTEGISSIRRDWIARRNDTYPI
RKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKKGIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEF
VREEVACV
>Mature_168_residues
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLTEGISSIRRDWIARRNDTYPI
RKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKKGIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEF
VREEVACV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=185, Percent_Identity=34.5945945945946, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 18866; Mature: 18866

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLT
CCCCHHHHCCCCCEEEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEHH
EGISSIRRDWIARRNDTYPIRKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKK
HHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHC
GIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEFVREEVACV
CCCCCCCEEEEEHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MHMNISEIYPSSNKIYINCTLYPDVKVGMRQINIRNSSQRFLLYDTAGPYTDCDIKVNLT
CCCCHHHHCCCCCEEEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEHH
EGISSIRRDWIARRNDTYPIRKAKTNSDARGVKALSGDDRVILKGIAGGAPVTQLFYAKK
HHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHC
GIITPEMEYVAVRENALLEQAEAIPGLLLPTKTRKITPEFVREEVACV
CCCCCCCEEEEEHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA