Click here to switch to the map view.

The map label for this gene is pbuE [H]

Identifier: 88196388

GI number: 88196388

Start: 2529341

End: 2530468

Strand: Reverse

Name: pbuE [H]

Synonym: SAOUHSC_02752

Alternate gene names: 88196388

Gene position: 2530468-2529341 (Counterclockwise)

Preceding gene: 88196389

Following gene: 88196385

Centisome position: 89.69

GC content: 35.2

Gene sequence:

>1128_bases
ATGATGGTTGCAGGAATTATGAACTTGATGAGTCAGGACTTACATGTATCAGAAGCTGTCGTTGGTCAATTAGTGACAAT
GTACGCTTTAACATTTGCGATATGTGGACCTATTCTGGTTAAATTAACGAACCGTTTTTCATCAAGGCCTGTATTATTAT
GGACATTACTTATATTTATCATTGGTAATGGCATTATTGCTGTAGCGCCAAATTTTTCAATATTAGTAGTTGGTAGAATT
ATCTCATCTGCAGCAGCAGCACTAATTATCGTAAAAGTATTAGCTATTACAGCGATGTTATCAGCACCTAAAAATCGTGG
TAAAATGATTGGACTTGTCTATACAGGGTTTAGTGGTGCTAATGTTTTTGGTGTACCAATTGGAACGGTTATCGGCGATT
TAGTAGGTTGGCGCTATACATTTCTATTCTTAATTATTGTGAGTATTATTGTTGGCTTCTTGATGATGATCTATTTACCG
AAGGATCAGGAAATACAACGAGGCCCTGTGAATCATGAGACACCATCTCATGAAAATCATGTTACTTCGAAAATATTAAG
ACCTGCTGAAGTAGCAAAATATTTAATTATTACGTTTTTAGTATTGATTGCAAACTCAGTGACATTCGTCTTTATAAATC
CACTTATTTTATCTAATGGTCATGATATGTCATTCGTTTCATTAGCACTTCTAGTAAATGGAATCGCTGGCGTTATTGGA
ACATCATTAGGTGGTATATTCTCCGATAAAATTACAAGTAAGCGTTGGTTAATGATTTCTGTTTCTATTTTTATCGTCAT
GATGTTACTTATGAATTTAATCTTACCTGGTTCAGGTCTATTGTTAGCAGGACTATTTATTTGGAATATCATGCAATGGA
GTACTAATCCAGCAGTGCAAAGCGGTGTGATTCAACATGTTGAAGGCGACACAAGCCAAGTAATGAGTTGGAACATGTCT
AGTTTAAACGCTGGTATTGGTGTTGGAGGCATTATTGGAGGCTTGGTCATGACACATGTTTCTGTTCAAGCTATCACATA
TACGAGTGCCATCATTGGCGCATTAGGATTAATCGTTGTTTTCACATTGAAAAATAATCATTATGCTAAAACATTTAAAT
CATCATAA

Upstream 100 bases:

>100_bases
GAAATGAATATCGGAAATTTTGTTTGAAAGGAGTTTTAAATTGTCAATCATGCGACTATTTACATTCATTTTAAGTATTT
TTATCGTAGGAATGGTTGAA

Downstream 100 bases:

>100_bases
TTCTCATATGAAAAGCACGCCTGCTATCAAATTCAGGTGTGCTTTTTTAGATGCGATAACGTTATTGATATGTGCGATAA
TAGCGACGTTCATTATGATA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRI
ISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLP
KDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIG
TSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMS
SLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

Sequences:

>Translated_375_residues
MMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRI
ISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLP
KDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIG
TSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMS
SLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS
>Mature_375_residues
MMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRI
ISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLP
KDQEIQRGPVNHETPSHENHVTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIG
TSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQVMSWNMS
SLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVFTLKNNHYAKTFKSS

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786595, Length=345, Percent_Identity=22.3188405797101, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787947, Length=250, Percent_Identity=27.2, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI145693198, Length=239, Percent_Identity=26.3598326359833, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787808, Length=357, Percent_Identity=26.890756302521, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40246; Mature: 40246

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFI
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGA
HCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
NVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENH
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHH
VTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIG
HHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHH
TSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQ
HHHCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHH
SGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVV
HHHHHHCCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
FTLKNNHYAKTFKSS
EEECCCCCCCCCCCC
>Mature Secondary Structure
MMVAGIMNLMSQDLHVSEAVVGQLVTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFI
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IGNGIIAVAPNFSILVVGRIISSAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGA
HCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
NVFGVPIGTVIGDLVGWRYTFLFLIIVSIIVGFLMMIYLPKDQEIQRGPVNHETPSHENH
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHH
VTSKILRPAEVAKYLIITFLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIG
HHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHH
TSLGGIFSDKITSKRWLMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQ
HHHCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHH
SGVIQHVEGDTSQVMSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVV
HHHHHHCCCCHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
FTLKNNHYAKTFKSS
EEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]