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The map label for this gene is alsS [H]

Identifier: 88196120

GI number: 88196120

Start: 2291064

End: 2292728

Strand: Reverse

Name: alsS [H]

Synonym: SAOUHSC_02468

Alternate gene names: 88196120

Gene position: 2292728-2291064 (Counterclockwise)

Preceding gene: 88196122

Following gene: 88196119

Centisome position: 81.26

GC content: 34.77

Gene sequence:

>1665_bases
ATGACTGATAAAAAGTACACTGCAGCCGATATGGTTATTGATACTTTGAAAAATAATGGGGTAGAATATGTTTTTGGTAT
TCCGGGTGCAAAGATTGACTATCTATTTAATGCTTTAATTGATGATGGTCCTGAACTTATTGTCACTCGTCATGAACAAA
ACGCCGCAATGATGGCGCAAGGTATTGGAAGATTAACAGGCAAACCGGGTGTAGTACTTGTTACAAGTGGTCCTGGTGTA
AGTAATTTAACTACTGGATTATTAACCGCAACATCTGAAGGGGATCCTGTATTAGCGTTAGGTGGCCAAGTGAAACGTAA
TGATTTATTACGATTAACGCATCAAAGTATTGATAATGCTGCGCTATTAAAATATTCATCAAAATATAGTGAAGAAGTAC
AAGATCCTGAATCATTATCAGAAGTTATGACAAATGCAATTCGAATTGCTACTTCAGGAAAAAATGGCGCAAGTTTTATT
AGTATTCCGCAAGACGTTATTTCTTCACCAGTTGAATCTAAAGCTATATCACTTTGCCAAAAAACAAATTTAGGAGTACC
GAGTGAACAAGATATTAATGATGTCATTGAAGCGATTAAAAATGCATCATTTCCTGTTTTATTAGCTGGTATGAGAAGTT
CAAGTGCAGAAGAAACAAATGCCATTCGCAAATTAGTTGAGCGCACGAATTTACCAGTTGTAGAAACATTCCAAGGTGCA
GGTGTAATTAGTCGTGAATTAGAAAATCATTTCTTCGGTCGTGTGGGCTTATTCCGCAATCAAGTTGGTGATGAATTATT
ACGTAAAAGTGATTTAGTTGTTACAATCGGTTATGATCCAATTGAATACGAAGCTAGTAACTGGAATAAAGAATTAGAAA
CACAAATTATCAATATTGACGAAGTTCAAGCTGAAATTACTAATTATATGCAACCGAAAAAAGAGTTGATTGGTAATATT
GCTAAAACGATTGAAATGATTTCTGAAAAAGTGGATGAGCCATTTATAAATCAACAACATTTAGACGAATTAGAACAATT
AAGAACACATATTGATGAAGAAACTGGTATTAAAGCGACGCATGAAGAAGGAATTCTACATCCAGTGGAAATTATTGAAT
CTATGCAAAAGGTATTAACTGATGATACTACTGTAACAGTTGATGTTGGAAGTCACTATATTTGGATGGCACGTAATTTC
AGAAGTTACAATCCAAGACATTTATTATTTAGCAATGGTATGCAAACGCTTGGTGTAGCATTACCGTGGGCAATTTCAGC
TGCACTTGTGCGCCCTAATACGCAAGTTGTGTCCGTTGCTGGCGATGGTGGCTTTTTATTTTCATCACAAGATTTAGAAA
CGGCCGTACGTAAAAATTTAAATATCATCCAGCTTATTTGGAATGATGGAAAATATAACATGGTTGAATTCCAAGAAGAA
ATGAAATATAAACGTTCGTCAGGTGTAGACTTCGGTCCTGTAGATTTTGTAAAATATGCAGAATCATTTGGCGCGAAAGG
TTTACGAGTTACTAATCAAGAAGAATTAGAAGCGGCAATTAAAGAGGGCTATGAAACAGATGGTCCAGTATTAATTGATA
TACCTGTAAATTACAAAGATAATATCAAACTTTCAACAAATATGTTACCTGACGTATTTAACTAA

Upstream 100 bases:

>100_bases
AACATCATTAATTTTGCGTAACTATCATTAGATTACAAATCACAAAGTAATTACATGTAATACACATCTATACATCACAT
TTGAAGGGAAATGAATATAA

Downstream 100 bases:

>100_bases
AATAAAGATAAATGTTAAAGAGGAGTGGGAGATTTTATGACTAATGTTTTATACCAACATGGTACATTAGGCACATTAAT
GGCAGGATTATTAAAAGGAA

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGV
SNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
SIPQDVISSPVESKAISLCQKTNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGA
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNI
AKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNF
RSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN

Sequences:

>Translated_554_residues
MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGV
SNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
SIPQDVISSPVESKAISLCQKTNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGA
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNI
AKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNF
RSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN
>Mature_553_residues
TDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVS
NLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFIS
IPQDVISSPVESKAISLCQKTNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGAG
VISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIA
KTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNFR
SYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEEM
KYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=27.8368794326241, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI21361361, Length=582, Percent_Identity=22.3367697594502, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1790104, Length=548, Percent_Identity=27.007299270073, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87081685, Length=552, Percent_Identity=27.1739130434783, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1787096, Length=543, Percent_Identity=26.7034990791897, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1786717, Length=573, Percent_Identity=25.4799301919721, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1788716, Length=566, Percent_Identity=23.4982332155477, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=551, Percent_Identity=25.5898366606171, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=552, Percent_Identity=25.5434782608696, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=563, Percent_Identity=24.6891651865009, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=578, Percent_Identity=28.719723183391, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=483, Percent_Identity=24.4306418219462, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=27.2727272727273, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61174; Mature: 61043

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQ
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
GIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNA
HHHHCCCCCCEEEEECCCCCCHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCH
ALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFISIPQDVISSPVESKAISLCQ
HHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHCCCHHHHHHHHHH
KTNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGA
HCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCC
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHEEECHH
EVQAEITNYMQPKKELIGNIAKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCC
HEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHH
LPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
HHHHHHHHEECCCCEEEEEECCCCEEECCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKD
HHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCEEEEEECCCCC
NIKLSTNMLPDVFN
CEEEECCCCCCCCC
>Mature Secondary Structure 
TDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQ
CCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHH
GIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNA
HHHHCCCCCCEEEEECCCCCCHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCH
ALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFISIPQDVISSPVESKAISLCQ
HHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCCEEEECCHHHHCCCHHHHHHHHHH
KTNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGA
HCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCC
GVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINID
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHEEECHH
EVQAEITNYMQPKKELIGNIAKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKAT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCC
HEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVA
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHH
LPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEE
HHHHHHHHEECCCCEEEEEECCCCEEECCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHH
MKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKD
HHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCEEEEEECCCCC
NIKLSTNMLPDVFN
CEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]