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The map label for this gene is lacR [H]

Identifier: 88196108

GI number: 88196108

Start: 2279892

End: 2280683

Strand: Reverse

Name: lacR [H]

Synonym: SAOUHSC_02456

Alternate gene names: 88196108

Gene position: 2280683-2279892 (Counterclockwise)

Preceding gene: 88196110

Following gene: 88196107

Centisome position: 80.84

GC content: 34.22

Gene sequence:

>792_bases
ATGACCCATCGCCTAAATAAAGGAGGCAATTTTACATTGAATAAACATGAACGTTTGGACGAAATTGCTAAACTAGTGAA
TAAAAAGGGCACGATAAGAACGAATGAAATCGTCGAAGGTTTAAATGTGTCTGATATGACAGTTCGAAGAGATTTAATTG
AATTGGAAAATAAAGGGATTTTAACGAAGATTCATGGTGGTGCACGCAGTAATTCAACATTTCAGTATAAAGAAATATCG
CATAAAGAAAAACATACCCGTCAAATTGCAGAAAAACGATTTATAGCTAAAAAAGCTGCATCATTAATTGAAGATGGGGA
TACTTTGTTTTTCGGACCAGGAACAACAGTGGAACTATTAGCAGAAGAAGTCAATCATCATACGCTCACAATTATTACGA
ATTGTTTGCCGGTGTATAAAATTTTGTTAGAAAAACAAACAGCACATTTTCGTGTCTATTTAATTGGTGGTGAAATGCGC
CATATTACAGAAGCATTTGTAGGTGAAATGGCGAACGCCATGTTGGAAAAACTAAGATTTAGCAAGATGTTCTTTAGTAG
TAACGCAGTAAATAAAGGTGCTGTGATGACATCTACATTAGATGAAGCCTATACGCAACAACTGGCACTAAGTAATTCAA
TTGAAAAATACTTGTTAATCGATCATACGAAAGTTGGCAAAGAAGATTTTACATCATTTTGTCAGTTAAACGAATTGACT
GCAGTGGTCATGGACTATGAAGATGAAGAAAAAGTAGAAACGATTAAAACATATATTGAAGTAGTCGACTAA

Upstream 100 bases:

>100_bases
TTATACATATAATCGAACAGTTAAATTATATTAGTTTTTACGCAATTTTTATTATAGAATAAGCATGTATATTAAAATTG
ATGTCTAAACATAAAGGATA

Downstream 100 bases:

>100_bases
ATTAAAAGTATAACTGCATTGTTAAAATATGTATCTATATGCTCATAAGTGAATGTGAATTTGCATTCATTTATGGGCTA
TTTTTTATGCAAATTTATGT

Product: lactose phosphotransferase system repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEIS
HKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELT
AVVMDYEDEEKVETIKTYIEVVD

Sequences:

>Translated_263_residues
MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEIS
HKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELT
AVVMDYEDEEKVETIKTYIEVVD
>Mature_262_residues
THRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISH
KEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRH
ITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELTA
VVMDYEDEEKVETIKTYIEVVD

Specific function: Repressor of the lactose catabolism operon. Galactose-6- phosphate is the inducer [H]

COG id: COG1349

COG function: function code KG; Transcriptional regulators of sugar metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH deoR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789829, Length=243, Percent_Identity=32.0987654320988, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082344, Length=255, Percent_Identity=30.5882352941176, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI226510968, Length=230, Percent_Identity=29.5652173913043, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789059, Length=235, Percent_Identity=26.3829787234043, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1789519, Length=190, Percent_Identity=31.5789473684211, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787063, Length=251, Percent_Identity=26.6932270916335, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788069, Length=232, Percent_Identity=25.4310344827586, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789170, Length=251, Percent_Identity=26.2948207171315, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790635, Length=217, Percent_Identity=25.3456221198157, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787540, Length=259, Percent_Identity=26.6409266409266, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014036
- InterPro:   IPR001034
- InterPro:   IPR018356
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00455 DeoR; PF08220 HTH_DeoR [H]

EC number: NA

Molecular weight: Translated: 29889; Mature: 29757

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00894 HTH_DEOR_1 ; PS51000 HTH_DEOR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGI
CCCCCCCCCCEEECHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHCCCCC
LTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELL
EEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHH
AEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMANAMLEKLRF
HHHHCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELT
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHHE
AVVMDYEDEEKVETIKTYIEVVD
EEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
THRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVSDMTVRRDLIELENKGI
CCCCCCCCCEEECHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHCCCCC
LTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIAKKAASLIEDGDTLFFGPGTTVELL
EEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHH
AEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMRHITEAFVGEMANAMLEKLRF
HHHHCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
SKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYLLIDHTKVGKEDFTSFCQLNELT
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCHHHHHHHHHHHHHE
AVVMDYEDEEKVETIKTYIEVVD
EEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2163387 [H]