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The map label for this gene is hsdM [H]

Identifier: 88195628

GI number: 88195628

Start: 1840527

End: 1842266

Strand: Reverse

Name: hsdM [H]

Synonym: SAOUHSC_01933

Alternate gene names: 88195628

Gene position: 1842266-1840527 (Counterclockwise)

Preceding gene: 88195629

Following gene: 88195627

Centisome position: 65.3

GC content: 37.76

Gene sequence:

>1740_bases
ATGATTTTGAAAGCATTTGAAAGCTACAACATTTCTATAAAATTTTTCAATAACAATTGCGCCACTAAAACTCAAAATTT
CCACCACCAACATCCAAATTATCAACATCGCAACATAACCAAATGTTATAATAAATCTATTACACAAAGAGATAAATTAC
TTATGCAAAGGCGGAGGAATCACATGTCTATTACTGAAAAACAACGTCAGCAACAAGCTGAATTACATAAAAAATTATGG
TCGATTGCGAATGATTTAAGAGGGAACATGGATGCGAGTGAATTCCGTAATTACATTTTAGGCTTGATTTTCTATCGCTT
CTTATCTGAAAAAGCCGAACAAGAATATGCAGATGCCTTGTCAGGTGAAGACATCACGTATCAAGAAGCATGGGCAGATG
AAGAATATCGTGAAGACTTAAAAGCAGAATTAATTGATCAAGTCGGTTACTTCATTGAGCCACAAGATTTATTCAGTGCG
ATGATTCGTGAAATTGAAACGCAAGATTTCGATATAGAACATCTAGCGACGGCGATTCGCAAAGTTGAAACATCTACATT
AGGTGAAGAAAGTGAAAATGACTTTATCGGGCTGTTCAGCGATATGGATTTGAGTTCAACGCGACTAGGTAACAATGTCA
AAGAACGTACTGCTTTAATCTCTAAAGTCATGGTTAATCTTGACGACTTACCATTCGTTCACAGTGACATGGAAATTGAT
ATGTTAGGTGATGCATATGAATTCCTAATCGGGCGCTTTGCGGCGACAGCGGGTAAAAAAGCAGGCGAGTTCTATACACC
ACAACAAGTATCTAAGATACTGGCGAAGATTGTCACAGACGGTAAAGATAAATTACGTCACGTGTACGACCCAACATGTG
GTTCCGGTTCATTGTTGTTACGTGTTGGTAAAGAAACGCAAGTGTATCGTTATTTCGGACAAGAACGTAACAATACTACA
TACAACTTAGCACGCATGAATATGTTATTACATGATGTGCGTTATGAGAACTTCGAGATCCGTAATGATGACACATTGGA
AAATCCAGCCTTTTTAGGCAATACATTTGATGCGGTTATTGCGAACCCACCATACAGTGCGAAATGGACAGCAGATTCAA
AATTTGAAAATGATGAACGATTCAGTGGTTACGGCAAGCTTGCGCCAAAATCCAAAGCAGACTTTGCCTTTATTCAACAC
ATGGTACATTACCTAGACGATGAAGGTACCATGGCAGTCGTACTCCCACATGGTGTATTATTCCGTGGTGCCGCAGAAGG
TGTGATTCGTCGCTATTTAATAGAAGAAAAGAACTACTTAGAAGCCGTGATTGGGTTACCAGCCAATATTTTCTATGGGA
CAAGTATTCCAACATGTATTTTAGTATTTAAAAAATGTCGCCAACAAGACGACAACGTACTATTTATCGATGCATCCAAT
GATTTTGAAAAAGGAAAAAATCAAAACCATTTAAGCGATGCCCAAGTCGAACGTATTATAGACACATATAAGCGTAAAGC
AACGATTGATAAATATAGCTACAGCGCGACATTACAAGAGATCGCCGATAACGATTACAACTTAAACATACCGCGATATG
TTGATACATTCGAAGAAGAAGCACCAATTGATTTAGATCAAGTCCAACAAGATTTGAAAAATATCGACAAAGAAATCGCA
GAAATTGAACAAGAAATCAATGCATACCTGAAAGAACTTGGGGTGTTGAAAGATGAGTAA

Upstream 100 bases:

>100_bases
TGCATCAAAGCCAATCAACGATTGATTTTCACCAACTCAATTGTTGATTGGTTTTATTTATGTATGAATGAACAACTTTT
TGACATCATTAAGAATATAA

Downstream 100 bases:

>100_bases
TACACAAACGAAAAATGTGCCAGAGTTGAGATTCCCAGGATTTGAAGGCGAATGGGAAGAGAAGAAGTTAGGGAATCTTA
CTACCAAAATAGGTAGTGGA

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLW
SIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSA
MIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTT
YNLARMNMLLHDVRYENFEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQH
MVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
DFEKGKNQNHLSDAQVERIIDTYKRKATIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIA
EIEQEINAYLKELGVLKDE

Sequences:

>Translated_579_residues
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLW
SIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSA
MIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTT
YNLARMNMLLHDVRYENFEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQH
MVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
DFEKGKNQNHLSDAQVERIIDTYKRKATIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIA
EIEQEINAYLKELGVLKDE
>Mature_579_residues
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRNHMSITEKQRQQQAELHKKLW
SIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSA
MIREIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTT
YNLARMNMLLHDVRYENFEIRNDDTLENPAFLGNTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQH
MVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
DFEKGKNQNHLSDAQVERIIDTYKRKATIDKYSYSATLQEIADNDYNLNIPRYVDTFEEEAPIDLDQVQQDLKNIDKEIA
EIEQEINAYLKELGVLKDE

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=501, Percent_Identity=25.1497005988024, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 66905; Mature: 66905

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRN
CCEECEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHC
HMSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADAL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIR
CCCCCCHHHHHCCHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHCCCCCCHHHHHHHHH
KVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
HHHHHHCCCCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLL
ECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEE
RVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFEIRNDDTLENPAFLGNTFDAVI
EECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHEE
ANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVL
CCCCCCCEECCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHH
FRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCC
DFEKGKNQNHLSDAQVERIIDTYKRKATIDKYSYSATLQEIADNDYNLNIPRYVDTFEEE
CHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
APIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MILKAFESYNISIKFFNNNCATKTQNFHHQHPNYQHRNITKCYNKSITQRDKLLMQRRRN
CCEECEECCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHC
HMSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFYRFLSEKAEQEYADAL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIREIETQDFDIEHLATAIR
CCCCCCHHHHHCCHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHCCCCCCHHHHHHHHH
KVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALISKVMVNLDDLPFVHSDMEID
HHHHHHCCCCCCCCEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEE
MLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVTDGKDKLRHVYDPTCGSGSLLL
ECCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEE
RVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYENFEIRNDDTLENPAFLGNTFDAVI
EECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHHEE
ANPPYSAKWTADSKFENDERFSGYGKLAPKSKADFAFIQHMVHYLDDEGTMAVVLPHGVL
CCCCCCCEECCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHH
FRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYGTSIPTCILVFKKCRQQDDNVLFIDASN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCC
DFEKGKNQNHLSDAQVERIIDTYKRKATIDKYSYSATLQEIADNDYNLNIPRYVDTFEEE
CHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCC
APIDLDQVQQDLKNIDKEIAEIEQEINAYLKELGVLKDE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]