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The map label for this gene is rot

Identifier: 88195576

GI number: 88195576

Start: 1794447

End: 1794848

Strand: Reverse

Name: rot

Synonym: SAOUHSC_01879

Alternate gene names: 88195576

Gene position: 1794848-1794447 (Counterclockwise)

Preceding gene: 88195577

Following gene: 88195573

Centisome position: 63.62

GC content: 31.34

Gene sequence:

>402_bases
ATGAAAAAAGTAAATAACGACACTGTATTTGGAATTTTGCAATTAGAAACACTTTTGGGTGACATTAACTCAATTTTCAG
CGAGATTGAAAGCGAATACAAAATGTCTAGAGAAGAAATTTTAATTTTACTAACTTTATGGCAAAAAGGTTCTATGACGC
TTAAAGAAATGGACAGATTTGTTGAAGTTAAACCGTATAAGCGTACGAGAACGTATAATAATTTAGTTGAATTAGAATGG
ATTTACAAAGAGCGTCCTGTTGACGATGAAAGAACAGTTATTATTCATTTCAATGAAAAGTTACAACAAGAGAAAGTAGA
GTTGTTGAATTTCATCAGTGATGCGATTGCAAGTAGAGCAACAGCAATGCAAAATAGTTTAAACGCAATTATTGCTGTGT
AA

Upstream 100 bases:

>100_bases
TATGCATAAGTTAGCACATACAAGTTTTGGGATTGTTGGGATGTTTGTTAATACTTGTATAGTAGCTAAATATGTGATTA
TTAATTGGGAGATGTTTAGC

Downstream 100 bases:

>100_bases
GTTTAATAGCATAAAAAGAGGTTTTCATTAAGTTGAAAACCTCTTTTTGTTGTTGGCATTAATTTTTCAAATGTTGACTA
CTCAATCCTAAATTATAAAT

Product: virulence factor regulator protein

Products: NA

Alternate protein names: Repressor of toxins

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEW
IYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSLNAIIAV

Sequences:

>Translated_133_residues
MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEW
IYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSLNAIIAV
>Mature_133_residues
MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEW
IYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRATAMQNSLNAIIAV

Specific function: Global regulator with both positive and negative effects that mediates modulation of several genes involved in virulence. Negatively regulates the transcription of several known virulence factors such as lipase (geh), hemolysins (hla and hlb) and protease

COG id: COG1846

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rot family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ROT_STAA8 (Q9RFJ6)

Other databases:

- EMBL:   AF189239
- EMBL:   CP000253
- RefSeq:   YP_500382.1
- ProteinModelPortal:   Q9RFJ6
- STRING:   Q9RFJ6
- EnsemblBacteria:   EBSTAT00000028144
- GeneID:   3921768
- GenomeReviews:   CP000253_GR
- KEGG:   sao:SAOUHSC_01879
- eggNOG:   COG1846
- GeneTree:   EBGT00050000023836
- HOGENOM:   HBG692942
- ProtClustDB:   CLSK885542
- BioCyc:   SAUR93061:SAOUHSC_01879-MONOMER
- GO:   GO:0005622
- GO:   GO:0009405
- InterPro:   IPR010166
- InterPro:   IPR000835
- InterPro:   IPR016998
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PIRSF:   PIRSF032474
- TIGRFAMs:   TIGR01889

Pfam domain/function: PF01047 MarR

EC number: NA

Molecular weight: Translated: 15600; Mature: 15600

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLWQKGSMTLKEMDRF
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCHHHHHHH
VEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRA
HCCCCCHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
TAMQNSLNAIIAV
HHHHHHHHEEEEC
>Mature Secondary Structure
MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILLTLWQKGSMTLKEMDRF
CCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCCCHHHHHHH
VEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQQEKVELLNFISDAIASRA
HCCCCCHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
TAMQNSLNAIIAV
HHHHHHHHEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10809700; 12511508