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The map label for this gene is citZ [H]

Identifier: 88195502

GI number: 88195502

Start: 1702811

End: 1703668

Strand: Reverse

Name: citZ [H]

Synonym: SAOUHSC_01802

Alternate gene names: 88195502

Gene position: 1703668-1702811 (Counterclockwise)

Preceding gene: 88195504

Following gene: 88195501

Centisome position: 60.38

GC content: 36.95

Gene sequence:

>858_bases
GTGCATCCAATGACAGCATTACGTACGTCATTATCATATATTGCACATTTCGATCCTGATGCTGAAAATGAATCAGATGA
AAATCGTTATGAAAGAGCAATGCGTATACAGGCTAAAGTAGCATCATTAGTTACAGCGTTTGCTCGAGTAAGACAAGATA
AAGAACCACTTAAGCCTAATCCTGACTTAAGTTATGCGGCAAACTTCCTATATATGTTACGTGGGGAATTACCAACAGAT
ATAGAAGTAGAAGCCTTCAATAAAGCACTTATTTTACACGCTGATCATGAGTTGAACGCATCTGCATTTACGGCACGTTG
TGCGGTATCATCATTGTCAGATATGTACTCAGGTATTGTAGCAGCCGTAGGTTCTCTGAAAGGGCCATTACATGGTGGTG
CAAACGAACAAGTTATGACGATGTTATCTGAGATTGGGTCAATTGAAAATGTTGATGCTTACTTAGATGAAAAATTTGCT
AATAAAGATAAAGTAATGGGCTTCGGTCATCGTGTATATAAAGATGGTGATCCTAGAGCGAAATATTTAAGAGAAATGAG
CCGTCAAATTACGAAAGACGCTGGTCGTGAAGAATTATTTGAAATGTCAGTGAAAATGGAAAAACGTATGGCAGAAGAAA
AAGGATTAATTCCTAATGTTGATTTTTATAGTGCGAGTGTTTATCACTGTATGGAAATACCTCATGACTTATTCACGCCA
ATCTTTGCTGTAAGTCGTTCTGCAGGATGGATTGCTCATATTTTAGAACAATATAAAGATAATAGAATTATGCGTCCTAG
AGCGAAATATATTGGCGAAACGAATCGTAAGTATATCCCGCTTGAAGAAAGAAAATAA

Upstream 100 bases:

>100_bases
AAACGAAGAAGAGCTTGCTCATCTAAAAGGGAAATTAAATCAATACATGACATTAAATCCTCGTGTGTATACACATTTTG
AGGAGTATGTTACAGATCAC

Downstream 100 bases:

>100_bases
TCAATACAAATTAAAAATGAAGATGTAAAATTTGGAGGTAAAATAACTATGACTGCAGAAAAAATTACTCAAGGAACTGA
AGGATTAAACGTACCTAATG

Product: hypothetical protein

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MHPMTALRTSLSYIAHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYMLRGELPTD
IEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPLHGGANEQVMTMLSEIGSIENVDAYLDEKFA
NKDKVMGFGHRVYKDGDPRAKYLREMSRQITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTP
IFAVSRSAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK

Sequences:

>Translated_285_residues
MHPMTALRTSLSYIAHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYMLRGELPTD
IEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPLHGGANEQVMTMLSEIGSIENVDAYLDEKFA
NKDKVMGFGHRVYKDGDPRAKYLREMSRQITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTP
IFAVSRSAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK
>Mature_285_residues
MHPMTALRTSLSYIAHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYMLRGELPTD
IEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPLHGGANEQVMTMLSEIGSIENVDAYLDEKFA
NKDKVMGFGHRVYKDGDPRAKYLREMSRQITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTP
IFAVSRSAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=283, Percent_Identity=27.9151943462898, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1786527, Length=285, Percent_Identity=35.0877192982456, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1786939, Length=292, Percent_Identity=37.6712328767123, Blast_Score=182, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17555174, Length=247, Percent_Identity=29.1497975708502, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324328, Length=291, Percent_Identity=24.7422680412371, Blast_Score=76, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319850, Length=254, Percent_Identity=25.9842519685039, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325257, Length=181, Percent_Identity=32.0441988950276, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356863, Length=242, Percent_Identity=29.3388429752066, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640124, Length=282, Percent_Identity=28.7234042553192, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640126, Length=282, Percent_Identity=28.7234042553192, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 32392; Mature: 32392

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPMTALRTSLSYIAHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPN
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDLSYAANFLYMLRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIV
CCHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
AAVGSLKGPLHGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRA
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCHHHCCCCCHHH
KYLREMSRQITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHH
IFAVSRSAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK
HHHHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCEECCCCCCC
>Mature Secondary Structure
MHPMTALRTSLSYIAHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPN
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDLSYAANFLYMLRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIV
CCHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
AAVGSLKGPLHGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRA
HHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHCCHHHCCCCCHHH
KYLREMSRQITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTP
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHH
IFAVSRSAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK
HHHHHCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]