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The map label for this gene is hmp [H]

Identifier: 88194014

GI number: 88194014

Start: 226620

End: 227765

Strand: Reverse

Name: hmp [H]

Synonym: SAOUHSC_00204

Alternate gene names: 88194014

Gene position: 227765-226620 (Counterclockwise)

Preceding gene: 88194018

Following gene: 88194013

Centisome position: 8.07

GC content: 34.82

Gene sequence:

>1146_bases
ATGCTTACAGAACAAGAGAAAGACATTATCAAACAAACGGTGCCTTTACTTAAAGAGAAAGGGACAGAAATTACGTCAAT
CTTTTATCCAAAAATGTTTAAAGCGCATCCTGAACTTTTAAACATGTTTAATCAAACGAACCAAAAACGAGGCATGCAAT
CTTCAGCATTAGCACAAGCTGTAATGGCCGCAGCGGTTAATATCGATAACTTAAGTGTTATTAAACCAGTCATTATGCCA
GTCGCATATAAACACTGCGCACTACAAGTTTATGCTGAACATTATCCAATTGTGGGGAAAAATTTATTAAAAGCCATTCA
AGACGTGACAGGATTAGAAGAAAATGACCCTGTCATTCAAGCTTGGGCAAAAGCATATGGCGTTATTGCGGATGTGTTCA
TCCAAATTGAAAAAGAAATTTATGATCAAATGATGTGGATTGGTTTTAAACCATTCAAAATTACCAATATTAAACAAGAA
TCTGAAGACATTAAATCATTTACAGTTGAAACTGAAGAATATGACTTTAGTGAATTTACACCAGGCCAATACATCACAGT
TGATGTTTCTAGTGATAAACTTCCATATAGAGCTAAACGTCACTATTCTATCGTATCAGGTGAAAAAAACCATTTAACTT
TTGGCGTTAAACGTGATGTCACAACAGAACATGAAGGCGAAGTTTCAACAATTTTACACGATGAGATTAAAGAAGGCGAT
ATGATTAATTTAGCTGCGCCTGTAGGTGGATTCGTATTAGAGAATACGACTGAACCACAACTTTTCTTAGGTTCAGGTAT
TGGTGTTACACCTTTAGTAGCTATGTATGAAGCTGCCTCTGCCAAAGGTTTAGATACACAGATGGTTCAAGTTGCTGAAA
ATGAACAACATTTACCTTTCAAAGACAACTTCAACAGTATCGCAAGCCATCATGACAACGCTAAATTATATACACACTTA
AAAGATAAACAAGGCTATATTGGTGCTGAAGAATTACAAGTATTTTTAGCAAATAAACCTGAAATTTATATCTGTGGTGG
TACAAAATTCTTACAATCTATGATCGAAGCACTTAAATCTTTAAATTACGATATGGATCGCGTACACTACGAAACATTTA
TTCCAAGACTAAGCGTTGCAGTATAA

Upstream 100 bases:

>100_bases
ATCAAATTTGCCATTTTATTTATATTTTCTCAAACCAATTAATTGAATATCGAAATTTTTAGTAGAATAATCAAAATATA
CAGATTAAAGGAGGAGTATC

Downstream 100 bases:

>100_bases
CTGAGAAAAGAGGTTTAGTGCTCATATGAAACTTAATCAACGTTACGTAAAAGTATTTGCATTATATTTCGTAAGTATTG
TTACTGCAAATATTATTGTT

Product: globin domain-containing protein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFNQTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMP
VAYKHCALQVYAEHYPIVGKNLLKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKRDVTTEHEGEVSTILHDEIKEGD
MINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMYEAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHL
KDKQGYIGAEELQVFLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV

Sequences:

>Translated_381_residues
MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFNQTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMP
VAYKHCALQVYAEHYPIVGKNLLKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKRDVTTEHEGEVSTILHDEIKEGD
MINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMYEAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHL
KDKQGYIGAEELQVFLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV
>Mature_381_residues
MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFNQTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMP
VAYKHCALQVYAEHYPIVGKNLLKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKRDVTTEHEGEVSTILHDEIKEGD
MINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMYEAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHL
KDKQGYIGAEELQVFLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=397, Percent_Identity=31.7380352644836, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788104, Length=222, Percent_Identity=22.0720720720721, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321673, Length=396, Percent_Identity=41.4141414141414, Blast_Score=258, Evalue=9e-70,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR001834
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 42908; Mature: 42908

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFNQTNQKRGMQSSALAQA
CCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHH
VMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNLLKAIQDVTGLEENDPVIQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHH
AWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQESEDIKSFTVETEEYDFSEFT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHEEEECCCCCCCCCC
PGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKRDVTTEHEGEVSTILHDEIKEGD
CCCEEEEEECCCCCCCHHHHCEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCC
MINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMYEAASAKGLDTQMVQVAENEQHLPF
EEEEECCCCCEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
KDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQVFLANKPEIYICGGTKFLQSMIEALKS
CCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHEEECCCCEEEEECCHHHHHHHHHHHHH
LNYDMDRVHYETFIPRLSVAV
CCCCHHHHHHHHHHCHHHHCC
>Mature Secondary Structure
MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFNQTNQKRGMQSSALAQA
CCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHH
VMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGKNLLKAIQDVTGLEENDPVIQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHH
AWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQESEDIKSFTVETEEYDFSEFT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHEEEECCCCCCCCCC
PGQYITVDVSSDKLPYRAKRHYSIVSGEKNHLTFGVKRDVTTEHEGEVSTILHDEIKEGD
CCCEEEEEECCCCCCCHHHHCEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCC
MINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMYEAASAKGLDTQMVQVAENEQHLPF
EEEEECCCCCEEECCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
KDNFNSIASHHDNAKLYTHLKDKQGYIGAEELQVFLANKPEIYICGGTKFLQSMIEALKS
CCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHEEECCCCEEEEECCHHHHHHHHHHHHH
LNYDMDRVHYETFIPRLSVAV
CCCCHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]