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The map label for this gene is thiG [H]

Identifier: 87201302

GI number: 87201302

Start: 3507383

End: 3508378

Strand: Direct

Name: thiG [H]

Synonym: Saro_3290

Alternate gene names: 87201302

Gene position: 3507383-3508378 (Clockwise)

Preceding gene: 87201301

Following gene: 87201305

Centisome position: 98.48

GC content: 66.47

Gene sequence:

>996_bases
ATGACCGGACAGCTATCCCTTACCGTCAACGGCGAACCCCGCCGCGCCGCGCCCGGATCGATCGCCGACCTGGTGCGCAG
CCTGGAACTCGATCCGGCCAAGGTCGCGGTCGAACGCAATGGCGAGATCGTCCCGCGCTCGACCTTGGCCAGCGTGGCGA
TCGCCGATGGGGACGTGCTGGAAATCGTGCATTTCGTGGGTGGAGGACAATCGGACGTGACCGACAACAACGATACCTGG
ACCGTCGCCGGACGCACCTTCACCTCGCGCCTGATCGTGGGCACGGGCAAGTACAAGGACTTCGAGCAGAACGCCGCCGC
GGTCGAAGCATCGGGCGCGGAGATCGTCACCGTCGCCGTGCGCAGGGTCAACGTCTCGGACCCCAAGGCGCCGATGCTGA
CCGACTACATCGACCCGAAGAAAATCACCTACCTGCCCAACACCGCCGGCTGCTTTACCGCCGAGGACGCGATCCGCACG
CTGCGCCTTGCGCGCGAGGCGGGCGGCTGGGATCTGGTGAAGCTGGAAGTCCTGGGCGAGGCGCGCACGCTCTATCCCAA
CATGATCGAAACGATCCGCGCGACCGAAGTCCTGGCCAAGGAAGGCTTCCTGCCAATGGTCTATTGCGTCGACGATCCGA
TCGCTGCCAAGCAGCTTGAAGACGCGGGCGCGGTCGCCGTCATGCCACTGGGCGCGCCGATCGGTTCGGGCCTCGGCATC
CAGAACAAGGTAACGGTGCGGCTGATCGTCGAAGGCGCCAAGGTGCCGGTGCTCGTCGACGCAGGCGTGGGCACCGCTTC
CGAAGCCGCCGTGGCGATGGAGCTTGGCTGCGATGGCGTGCTGATGAACACCGCCATCGCCGAGGCCAAGGACCCGATCC
GCATGGCCCGCGCAATGAAGCTGGCCGTTCAGGCCGGACGCGACGCCTATCTCGCCGGCCGCATGCCGACGCGCAAGTAC
GCCGATCCGTCGAGCCCGCTGGCCGGGTTGATCTGA

Upstream 100 bases:

>100_bases
TTGCGGCGTCGTGCTGGTGGCCGCCGCCCACGTCATCAACATGCGGCACGCGCACTAAGCGCCACTTGCGCCCGCACACT
GCGCCGCTAAAGCGGGCCGC

Downstream 100 bases:

>100_bases
GGATCGAGGCCCCCCGCTTTCGCGGGGAGCGCCCCATCCCGCTTGCGGATCAGCCGACGATGCCCGCCGCTGCCAGCACG
GCCAGGGTCAGGATGTCGGG

Product: bifunctional sulfur carrier protein/thiazole synthase protein

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTGQLSLTVNGEPRRAAPGSIADLVRSLELDPAKVAVERNGEIVPRSTLASVAIADGDVLEIVHFVGGGQSDVTDNNDTW
TVAGRTFTSRLIVGTGKYKDFEQNAAAVEASGAEIVTVAVRRVNVSDPKAPMLTDYIDPKKITYLPNTAGCFTAEDAIRT
LRLAREAGGWDLVKLEVLGEARTLYPNMIETIRATEVLAKEGFLPMVYCVDDPIAAKQLEDAGAVAVMPLGAPIGSGLGI
QNKVTVRLIVEGAKVPVLVDAGVGTASEAAVAMELGCDGVLMNTAIAEAKDPIRMARAMKLAVQAGRDAYLAGRMPTRKY
ADPSSPLAGLI

Sequences:

>Translated_331_residues
MTGQLSLTVNGEPRRAAPGSIADLVRSLELDPAKVAVERNGEIVPRSTLASVAIADGDVLEIVHFVGGGQSDVTDNNDTW
TVAGRTFTSRLIVGTGKYKDFEQNAAAVEASGAEIVTVAVRRVNVSDPKAPMLTDYIDPKKITYLPNTAGCFTAEDAIRT
LRLAREAGGWDLVKLEVLGEARTLYPNMIETIRATEVLAKEGFLPMVYCVDDPIAAKQLEDAGAVAVMPLGAPIGSGLGI
QNKVTVRLIVEGAKVPVLVDAGVGTASEAAVAMELGCDGVLMNTAIAEAKDPIRMARAMKLAVQAGRDAYLAGRMPTRKY
ADPSSPLAGLI
>Mature_330_residues
TGQLSLTVNGEPRRAAPGSIADLVRSLELDPAKVAVERNGEIVPRSTLASVAIADGDVLEIVHFVGGGQSDVTDNNDTWT
VAGRTFTSRLIVGTGKYKDFEQNAAAVEASGAEIVTVAVRRVNVSDPKAPMLTDYIDPKKITYLPNTAGCFTAEDAIRTL
RLAREAGGWDLVKLEVLGEARTLYPNMIETIRATEVLAKEGFLPMVYCVDDPIAAKQLEDAGAVAVMPLGAPIGSGLGIQ
NKVTVRLIVEGAKVPVLVDAGVGTASEAAVAMELGCDGVLMNTAIAEAKDPIRMARAMKLAVQAGRDAYLAGRMPTRKYA
DPSSPLAGLI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=250, Percent_Identity=45.6, Blast_Score=216, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 34799; Mature: 34668

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQLSLTVNGEPRRAAPGSIADLVRSLELDPAKVAVERNGEIVPRSTLASVAIADGDVL
CCCEEEEEECCCCCCCCCCHHHHHHHHCCCCHHEEEEECCCCCCCCHHHHEEEECCCCHH
EIVHFVGGGQSDVTDNNDTWTVAGRTFTSRLIVGTGKYKDFEQNAAAVEASGAEIVTVAV
HHHHHHCCCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEE
RRVNVSDPKAPMLTDYIDPKKITYLPNTAGCFTAEDAIRTLRLAREAGGWDLVKLEVLGE
EEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEECC
ARTLYPNMIETIRATEVLAKEGFLPMVYCVDDPIAAKQLEDAGAVAVMPLGAPIGSGLGI
CHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCC
QNKVTVRLIVEGAKVPVLVDAGVGTASEAAVAMELGCDGVLMNTAIAEAKDPIRMARAMK
CCEEEEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCEEEHHHHHHCCCHHHHHHHHH
LAVQAGRDAYLAGRMPTRKYADPSSPLAGLI
HHHHCCCCEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGQLSLTVNGEPRRAAPGSIADLVRSLELDPAKVAVERNGEIVPRSTLASVAIADGDVL
CCEEEEEECCCCCCCCCCHHHHHHHHCCCCHHEEEEECCCCCCCCHHHHEEEECCCCHH
EIVHFVGGGQSDVTDNNDTWTVAGRTFTSRLIVGTGKYKDFEQNAAAVEASGAEIVTVAV
HHHHHHCCCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEEE
RRVNVSDPKAPMLTDYIDPKKITYLPNTAGCFTAEDAIRTLRLAREAGGWDLVKLEVLGE
EEECCCCCCCCCCCCCCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEEECC
ARTLYPNMIETIRATEVLAKEGFLPMVYCVDDPIAAKQLEDAGAVAVMPLGAPIGSGLGI
CHHHCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCCCCC
QNKVTVRLIVEGAKVPVLVDAGVGTASEAAVAMELGCDGVLMNTAIAEAKDPIRMARAMK
CCEEEEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCEEEHHHHHHCCCHHHHHHHHH
LAVQAGRDAYLAGRMPTRKYADPSSPLAGLI
HHHHCCCCEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA