Click here to switch to the map view.

The map label for this gene is metF [H]

Identifier: 87201220

GI number: 87201220

Start: 3425534

End: 3426475

Strand: Reverse

Name: metF [H]

Synonym: Saro_3208

Alternate gene names: 87201220

Gene position: 3426475-3425534 (Counterclockwise)

Preceding gene: 87201221

Following gene: 87201219

Centisome position: 96.21

GC content: 67.09

Gene sequence:

>942_bases
ATGAACGCCAGCTACGACCAGCTCCGCGAGGCCCGCACCGCACTCGACACCCCGCTTTTTGCAGGGCTGCCGGGGGACAT
TGCCGTCTCGTTCGAGTTCTTCCCGCCAAAGAGCGATGCGCTGATGGCGCAGCTCTGGGATGTCGTCGAAACGCTGGCGC
CGCTACAGCCTACGTTCGTCTCCGTCACCTACGGCGCAGGTGGCTCAACGCGCGATCGCACTCACGGCACCGTTGCGCGG
ATCATCAAGGAGGCGAAGCTGCCAGCGGCCGCGCACCTCACTTGCGTGGACGCTAGCAAGGCCGAGATTCGCGAAGTCGC
CGAAAGCTACTGGGAAGCGGGCGTTCGCCATATCGTTGCCCTGCGCGGCGACATGGGTGCGCCCGGCGTGCCCTTCACTC
CCCACCCGGACGGCTATGCCAACGCGGCCGAACTCGTCGCCGGGCTGAAGCAGATCGCCCCGTTCGAGATCTCGGTCGCC
GCCTATCCGGAATCGCACCCGGACTCGCCGGACCAGCAGGCCGATATCGACAACCTCAAGCGCAAGCTCGATGCCGGGGC
CACGCGCGCAATCAGCCAGTTCTTCTTCGAGCCTGAAACCTTCTTCCGCTTCCGCGACAAGCTGGCGGCGGCCGGCATCG
ATGCCCCGGTCCTTCCCGGCATCCTGCCGGTTTCCAACGTCGCCCAGACGCGCAAGTTCGCCGCCGCCTGCGGTGCCGCG
ATCCCGGCGTGGATGGACGGCCTGTTCGAAGGGCTAGACACCCACCCCGCCTCGCGCCAGCTCGTCGCCGCGACTATCGC
CGCCGAGTTCTGCCGCCGCCTCTACGCCGGCGGCGTGCGCGATTTCCACTTCTACACCCTCAACCGCGCCGAACTGTCCT
ATGCGATCTGCCACCTGCTTGGCCTTCGTCCCCGGACTCCGGCGCTGGAGAAAGCCGCGTGA

Upstream 100 bases:

>100_bases
TGAACTCGTCGTCAAGCTGTGGACCGCAGTCCGCCGCGAAGACAGCGTCACCCAGCTTGAACCCCGTCAGAAATCCAGCT
CCGGAAAGACCTGAAGTCCC

Downstream 100 bases:

>100_bases
CCCTGATCCCTTCCCCCTTGATTCCTTCAGGCGCCCGCACCGCCTTTCTTGAACAGGCGAGCCAGCGCATCCTCATCACC
GATGGCGCGTTCGGAACCGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNASYDQLREARTALDTPLFAGLPGDIAVSFEFFPPKSDALMAQLWDVVETLAPLQPTFVSVTYGAGGSTRDRTHGTVAR
IIKEAKLPAAAHLTCVDASKAEIREVAESYWEAGVRHIVALRGDMGAPGVPFTPHPDGYANAAELVAGLKQIAPFEISVA
AYPESHPDSPDQQADIDNLKRKLDAGATRAISQFFFEPETFFRFRDKLAAAGIDAPVLPGILPVSNVAQTRKFAAACGAA
IPAWMDGLFEGLDTHPASRQLVAATIAAEFCRRLYAGGVRDFHFYTLNRAELSYAICHLLGLRPRTPALEKAA

Sequences:

>Translated_313_residues
MNASYDQLREARTALDTPLFAGLPGDIAVSFEFFPPKSDALMAQLWDVVETLAPLQPTFVSVTYGAGGSTRDRTHGTVAR
IIKEAKLPAAAHLTCVDASKAEIREVAESYWEAGVRHIVALRGDMGAPGVPFTPHPDGYANAAELVAGLKQIAPFEISVA
AYPESHPDSPDQQADIDNLKRKLDAGATRAISQFFFEPETFFRFRDKLAAAGIDAPVLPGILPVSNVAQTRKFAAACGAA
IPAWMDGLFEGLDTHPASRQLVAATIAAEFCRRLYAGGVRDFHFYTLNRAELSYAICHLLGLRPRTPALEKAA
>Mature_313_residues
MNASYDQLREARTALDTPLFAGLPGDIAVSFEFFPPKSDALMAQLWDVVETLAPLQPTFVSVTYGAGGSTRDRTHGTVAR
IIKEAKLPAAAHLTCVDASKAEIREVAESYWEAGVRHIVALRGDMGAPGVPFTPHPDGYANAAELVAGLKQIAPFEISVA
AYPESHPDSPDQQADIDNLKRKLDAGATRAISQFFFEPETFFRFRDKLAAAGIDAPVLPGILPVSNVAQTRKFAAACGAA
IPAWMDGLFEGLDTHPASRQLVAATIAAEFCRRLYAGGVRDFHFYTLNRAELSYAICHLLGLRPRTPALEKAA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=290, Percent_Identity=30.3448275862069, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI1790377, Length=293, Percent_Identity=53.5836177474403, Blast_Score=323, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=30.4964539007092, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=30.4964539007092, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=30.6122448979592, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321313, Length=287, Percent_Identity=27.5261324041812, Blast_Score=121, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33751; Mature: 33751

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASYDQLREARTALDTPLFAGLPGDIAVSFEFFPPKSDALMAQLWDVVETLAPLQPTFV
CCCCHHHHHHHHHHHCCCHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEE
SVTYGAGGSTRDRTHGTVARIIKEAKLPAAAHLTCVDASKAEIREVAESYWEAGVRHIVA
EEEECCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LRGDMGAPGVPFTPHPDGYANAAELVAGLKQIAPFEISVAAYPESHPDSPDQQADIDNLK
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHH
RKLDAGATRAISQFFFEPETFFRFRDKLAAAGIDAPVLPGILPVSNVAQTRKFAAACGAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IPAWMDGLFEGLDTHPASRQLVAATIAAEFCRRLYAGGVRDFHFYTLNRAELSYAICHLL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHH
GLRPRTPALEKAA
CCCCCCCCCCCCC
>Mature Secondary Structure
MNASYDQLREARTALDTPLFAGLPGDIAVSFEFFPPKSDALMAQLWDVVETLAPLQPTFV
CCCCHHHHHHHHHHHCCCHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEE
SVTYGAGGSTRDRTHGTVARIIKEAKLPAAAHLTCVDASKAEIREVAESYWEAGVRHIVA
EEEECCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LRGDMGAPGVPFTPHPDGYANAAELVAGLKQIAPFEISVAAYPESHPDSPDQQADIDNLK
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHH
RKLDAGATRAISQFFFEPETFFRFRDKLAAAGIDAPVLPGILPVSNVAQTRKFAAACGAA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IPAWMDGLFEGLDTHPASRQLVAATIAAEFCRRLYAGGVRDFHFYTLNRAELSYAICHLL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHH
GLRPRTPALEKAA
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA