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The map label for this gene is argF

Identifier: 87201152

GI number: 87201152

Start: 3350414

End: 3351349

Strand: Reverse

Name: argF

Synonym: Saro_3140

Alternate gene names: 87201152

Gene position: 3351349-3350414 (Counterclockwise)

Preceding gene: 87201153

Following gene: 87201151

Centisome position: 94.1

GC content: 66.35

Gene sequence:

>936_bases
GTGAGCCAGGCCCTCAAGCACTTCCTGAACCTGACGGATGCGGGCGGCGATGCCATCGCCGCGATGCTGAACGACGCGCT
CGACCGCAAGTCCGCGCGCGCTGGCCAGCCGAAGGGCAAGGCCGATGCCGACGCCCCTCTCGGCGGGCGGGTCCTGGCCA
TGGTTTTCGAGAAGAATTCGACCCGCACGCGCGTGTCATTCGACATCGCGATGCGCCAGCTCGGCGGTTCGGCGCTGATC
CTCGACGCGGGCACGACCCAGTTGGGCCGCGGCGAGACCATCGCCGATACCGCCCGCGTACTGAGCCGCATGGCCGATGC
CATCATGCTGCGGACGGACGACCACGCCAAGGTCGAGGAACTGGCGCGCCACGCTACCGTGCCGGTGATCAACGGGCTGA
CCGACCTGTCGCATCCCTGCCAGATCATGGCTGATCTTCTGACGGTGATCGAGCATGGCAAGGCCCTGCCCGGACTTGAG
GTGGCTTGGCTGGGCGACGGCAACAACGTGCTCAATTCGATCGTCGAGGCGGCGGGACTGATGAAGTTCAACGTGCGCAT
CGGCGTTCCCGAAGGCTATGAGTCCGATGCGGGAATGATCGAGGCCGCCGTTCTGGCCGGCGCCGGCATCCGTGTGATCC
GCGATCCGGTGGAAGCCGTGCGCGGGGCGGATGTCGTCGTGACCGACACCTGGATCTCGATGGGCCAGGCGCATGCCGAA
GCGAAGCTTGCGGCAATGGCGCCCTATCAGGTCAACGACGCGCTCATGGCCCACGCCAAGCCGGATGCGGTCTTCCTGCA
CTGCCTGCCCGCGCACCGCGGTGAGGAGGTGACCGATGCCGTCATCGACGGGCCGCGCTCGGTGGTCTGGGACGAGGCGG
AAAACCGTATCCATGCCCAGAAGTCGGTTCTGCGCTGGGTATTCGGACAGATTTGA

Upstream 100 bases:

>100_bases
GCGTGATCCCGCCGCTGGTCATCGACGACAGCCACATCGACGAATTCATGGAGCGGCTTTCGGCCGCTGCCGCAAGCTAT
GCGCCGGAGGCGCCGGTCAC

Downstream 100 bases:

>100_bases
GGACGATTGAATTGAACGAACAGGTCCAGCCTTCCACCGGGTTCGACCGGGTGCTGCATTTCACCTTGCCGGATCGCAAC
GCCCGCGGGCGCGCGGTCCG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSQALKHFLNLTDAGGDAIAAMLNDALDRKSARAGQPKGKADADAPLGGRVLAMVFEKNSTRTRVSFDIAMRQLGGSALI
LDAGTTQLGRGETIADTARVLSRMADAIMLRTDDHAKVEELARHATVPVINGLTDLSHPCQIMADLLTVIEHGKALPGLE
VAWLGDGNNVLNSIVEAAGLMKFNVRIGVPEGYESDAGMIEAAVLAGAGIRVIRDPVEAVRGADVVVTDTWISMGQAHAE
AKLAAMAPYQVNDALMAHAKPDAVFLHCLPAHRGEEVTDAVIDGPRSVVWDEAENRIHAQKSVLRWVFGQI

Sequences:

>Translated_311_residues
MSQALKHFLNLTDAGGDAIAAMLNDALDRKSARAGQPKGKADADAPLGGRVLAMVFEKNSTRTRVSFDIAMRQLGGSALI
LDAGTTQLGRGETIADTARVLSRMADAIMLRTDDHAKVEELARHATVPVINGLTDLSHPCQIMADLLTVIEHGKALPGLE
VAWLGDGNNVLNSIVEAAGLMKFNVRIGVPEGYESDAGMIEAAVLAGAGIRVIRDPVEAVRGADVVVTDTWISMGQAHAE
AKLAAMAPYQVNDALMAHAKPDAVFLHCLPAHRGEEVTDAVIDGPRSVVWDEAENRIHAQKSVLRWVFGQI
>Mature_310_residues
SQALKHFLNLTDAGGDAIAAMLNDALDRKSARAGQPKGKADADAPLGGRVLAMVFEKNSTRTRVSFDIAMRQLGGSALIL
DAGTTQLGRGETIADTARVLSRMADAIMLRTDDHAKVEELARHATVPVINGLTDLSHPCQIMADLLTVIEHGKALPGLEV
AWLGDGNNVLNSIVEAAGLMKFNVRIGVPEGYESDAGMIEAAVLAGAGIRVIRDPVEAVRGADVVVTDTWISMGQAHAEA
KLAAMAPYQVNDALMAHAKPDAVFLHCLPAHRGEEVTDAVIDGPRSVVWDEAENRIHAQKSVLRWVFGQI

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=296, Percent_Identity=42.5675675675676, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI18105007, Length=268, Percent_Identity=26.1194029850746, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=39.7553516819572, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1786469, Length=333, Percent_Identity=38.7387387387387, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI48994908, Length=296, Percent_Identity=26.3513513513513, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=29.153605015674, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=124, Percent_Identity=37.0967741935484, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322373, Length=316, Percent_Identity=34.8101265822785, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322331, Length=273, Percent_Identity=28.2051282051282, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24642586, Length=269, Percent_Identity=26.0223048327138, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_NOVAD (Q2G3J8)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_498409.1
- HSSP:   P96108
- ProteinModelPortal:   Q2G3J8
- SMR:   Q2G3J8
- STRING:   Q2G3J8
- GeneID:   3918182
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_3140
- NMPDR:   fig|48935.1.peg.698
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   AKFLHCL
- PhylomeDB:   Q2G3J8
- ProtClustDB:   PRK00779
- BioCyc:   NARO279238:SARO_3140-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33086; Mature: 32955

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 111-111 BINDING 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQALKHFLNLTDAGGDAIAAMLNDALDRKSARAGQPKGKADADAPLGGRVLAMVFEKNS
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
TRTRVSFDIAMRQLGGSALILDAGTTQLGRGETIADTARVLSRMADAIMLRTDDHAKVEE
CCEEEHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCHHHHH
LARHATVPVINGLTDLSHPCQIMADLLTVIEHGKALPGLEVAWLGDGNNVLNSIVEAAGL
HHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCC
MKFNVRIGVPEGYESDAGMIEAAVLAGAGIRVIRDPVEAVRGADVVVTDTWISMGQAHAE
EEEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
AKLAAMAPYQVNDALMAHAKPDAVFLHCLPAHRGEEVTDAVIDGPRSVVWDEAENRIHAQ
HHHHEECCCCCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCCCEECCHHHHHHHHH
KSVLRWVFGQI
HHHHHHHHHCC
>Mature Secondary Structure 
SQALKHFLNLTDAGGDAIAAMLNDALDRKSARAGQPKGKADADAPLGGRVLAMVFEKNS
CHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
TRTRVSFDIAMRQLGGSALILDAGTTQLGRGETIADTARVLSRMADAIMLRTDDHAKVEE
CCEEEHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCHHHHH
LARHATVPVINGLTDLSHPCQIMADLLTVIEHGKALPGLEVAWLGDGNNVLNSIVEAAGL
HHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCC
MKFNVRIGVPEGYESDAGMIEAAVLAGAGIRVIRDPVEAVRGADVVVTDTWISMGQAHAE
EEEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHH
AKLAAMAPYQVNDALMAHAKPDAVFLHCLPAHRGEEVTDAVIDGPRSVVWDEAENRIHAQ
HHHHEECCCCCCHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCCCEECCHHHHHHHHH
KSVLRWVFGQI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA