Click here to switch to the map view.

The map label for this gene is pyrB

Identifier: 87201073

GI number: 87201073

Start: 3279684

End: 3280709

Strand: Direct

Name: pyrB

Synonym: Saro_3061

Alternate gene names: 87201073

Gene position: 3279684-3280709 (Clockwise)

Preceding gene: 87201069

Following gene: 87201074

Centisome position: 92.08

GC content: 65.69

Gene sequence:

>1026_bases
ATGCAAAGCCAGAACACCCCAGCAGCGGGCCGCTATCCAGCGGGCGGCCTCGCCTTCCCGCACCGTGACCTGACCGGCAT
CGGCCAGCTTGCGCGCCACGAGATTCTCTACCTCCTCGACGAGGCGGAACAGTGGGTGGAACTGAACCGGCAGAGCCAGA
AGACGACCGATCTTCTCAACGGCCTGACGATTATCAACGCCTTCTTCGAGAACTCGACCCGCACGCTGCTGAGCTTCGAG
ATCGCGGGCAAAAGGCTGGGCGCGGACGTGGTCAACATGCACGCCGCCACCAGCAGCGTGAAGAAGGGCGAAACGCTGAT
CGACACGGCGATGACGCTCAATGCCATGCGCGCGGACGCAATCGTCATCCGTCACGCCAGTTCGGGCGCGGTGCGCCTCA
TCGCGGAAAAGGTGGACTGCCCGGTGCTGAATGCCGGCGACGGACAGCACGAGCACCCGACGCAGGCCCTGCTCGATGCG
CTGACGATGCGCCATGCGATGAAGCTGCCCGCAGGATCGGACCTCAATGGTCTGAAAGTGACGATCTGCGGGGACATCCT
GCACAGCCGCGTGGCGCGATCCAACATTCTGAGCCTGACCGCCCTGGGCGCGGAAGTCCGCGTATGCGCGCCTCCCGCGC
TGATGCCGGCCGAGATCGAGGCAATGGGCGTGACGCCCTTCCACGACTTCGACGCGGCGCTCAAAGGGGCCAACATCGTG
ATGATGCTGCGCCTTCAGCAGGAGCGCATGTCGGGACAGTTCATCCCCTCCCCGCGCGAGTATCGCCACCTCTACGGGTT
GACGCCCGAACGGCTGGCCAGGGCGGAGGCCGACGCTTTCGTGATGCATCCCGGGCCGATGAACCGCGGCATAGAGATCG
ATTCGACCGTGGCGGACCATCCCACGCGCAGCCTGATCACGCGGCAGGTCGAAATGGGTGTTGCCATCCGCATGGCCTGC
CTCGAGGTGCTGACGCGGCGGGCACGGCGCACCCCGGGCTGGGAAGCGGAAGGAGCCTCGGCATGA

Upstream 100 bases:

>100_bases
GGGCAAAGCTCATGGGTTCGACTCTATTGTGGACGACTCCCCAAGGCCAGCGGGAAACCGCTTGAGCCCGCTTCAACCCG
CGACTAGGGAACTGGTTTCA

Downstream 100 bases:

>100_bases
TCGCGCAGCCTCTCACGATCATAGGCGGCCGGCTGGTGCCGGGCTCGGGCGAGCCGGTCGCCGGGAACCTGCGCTGCGAG
GGCGGACGCATCGTCGCGCT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MQSQNTPAAGRYPAGGLAFPHRDLTGIGQLARHEILYLLDEAEQWVELNRQSQKTTDLLNGLTIINAFFENSTRTLLSFE
IAGKRLGADVVNMHAATSSVKKGETLIDTAMTLNAMRADAIVIRHASSGAVRLIAEKVDCPVLNAGDGQHEHPTQALLDA
LTMRHAMKLPAGSDLNGLKVTICGDILHSRVARSNILSLTALGAEVRVCAPPALMPAEIEAMGVTPFHDFDAALKGANIV
MMLRLQQERMSGQFIPSPREYRHLYGLTPERLARAEADAFVMHPGPMNRGIEIDSTVADHPTRSLITRQVEMGVAIRMAC
LEVLTRRARRTPGWEAEGASA

Sequences:

>Translated_341_residues
MQSQNTPAAGRYPAGGLAFPHRDLTGIGQLARHEILYLLDEAEQWVELNRQSQKTTDLLNGLTIINAFFENSTRTLLSFE
IAGKRLGADVVNMHAATSSVKKGETLIDTAMTLNAMRADAIVIRHASSGAVRLIAEKVDCPVLNAGDGQHEHPTQALLDA
LTMRHAMKLPAGSDLNGLKVTICGDILHSRVARSNILSLTALGAEVRVCAPPALMPAEIEAMGVTPFHDFDAALKGANIV
MMLRLQQERMSGQFIPSPREYRHLYGLTPERLARAEADAFVMHPGPMNRGIEIDSTVADHPTRSLITRQVEMGVAIRMAC
LEVLTRRARRTPGWEAEGASA
>Mature_341_residues
MQSQNTPAAGRYPAGGLAFPHRDLTGIGQLARHEILYLLDEAEQWVELNRQSQKTTDLLNGLTIINAFFENSTRTLLSFE
IAGKRLGADVVNMHAATSSVKKGETLIDTAMTLNAMRADAIVIRHASSGAVRLIAEKVDCPVLNAGDGQHEHPTQALLDA
LTMRHAMKLPAGSDLNGLKVTICGDILHSRVARSNILSLTALGAEVRVCAPPALMPAEIEAMGVTPFHDFDAALKGANIV
MMLRLQQERMSGQFIPSPREYRHLYGLTPERLARAEADAFVMHPGPMNRGIEIDSTVADHPTRSLITRQVEMGVAIRMAC
LEVLTRRARRTPGWEAEGASA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=36.0128617363344, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI2367364, Length=321, Percent_Identity=33.9563862928349, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790703, Length=203, Percent_Identity=33.0049261083744, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786469, Length=208, Percent_Identity=32.2115384615385, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=344, Percent_Identity=31.1046511627907, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=319, Percent_Identity=34.1692789968652, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24642586, Length=275, Percent_Identity=38.1818181818182, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_NOVAD (Q2G3S7)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_498330.1
- HSSP:   P96108
- ProteinModelPortal:   Q2G3S7
- SMR:   Q2G3S7
- STRING:   Q2G3S7
- GeneID:   3916675
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_3061
- NMPDR:   fig|48935.1.peg.1488
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   NARO279238:SARO_3061-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 37079; Mature: 37079

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSQNTPAAGRYPAGGLAFPHRDLTGIGQLARHEILYLLDEAEQWVELNRQSQKTTDLLN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLTIINAFFENSTRTLLSFEIAGKRLGADVVNMHAATSSVKKGETLIDTAMTLNAMRADA
HHHHHHHHHCCCCCEEEEEEECCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCE
IVIRHASSGAVRLIAEKVDCPVLNAGDGQHEHPTQALLDALTMRHAMKLPAGSDLNGLKV
EEEEECCCCCEEEEEHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
TICGDILHSRVARSNILSLTALGAEVRVCAPPALMPAEIEAMGVTPFHDFDAALKGANIV
EEEHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHCCCCEE
MMLRLQQERMSGQFIPSPREYRHLYGLTPERLARAEADAFVMHPGPMNRGIEIDSTVADH
EEEEEHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCEEECCCCCCC
PTRSLITRQVEMGVAIRMACLEVLTRRARRTPGWEAEGASA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQSQNTPAAGRYPAGGLAFPHRDLTGIGQLARHEILYLLDEAEQWVELNRQSQKTTDLLN
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLTIINAFFENSTRTLLSFEIAGKRLGADVVNMHAATSSVKKGETLIDTAMTLNAMRADA
HHHHHHHHHCCCCCEEEEEEECCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCE
IVIRHASSGAVRLIAEKVDCPVLNAGDGQHEHPTQALLDALTMRHAMKLPAGSDLNGLKV
EEEEECCCCCEEEEEHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
TICGDILHSRVARSNILSLTALGAEVRVCAPPALMPAEIEAMGVTPFHDFDAALKGANIV
EEEHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHCCCCEE
MMLRLQQERMSGQFIPSPREYRHLYGLTPERLARAEADAFVMHPGPMNRGIEIDSTVADH
EEEEEHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCEEECCCCCCC
PTRSLITRQVEMGVAIRMACLEVLTRRARRTPGWEAEGASA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA