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The map label for this gene is gatA [H]

Identifier: 87201069

GI number: 87201069

Start: 3272946

End: 3274487

Strand: Direct

Name: gatA [H]

Synonym: Saro_3057

Alternate gene names: 87201069

Gene position: 3272946-3274487 (Clockwise)

Preceding gene: 87201065

Following gene: 87201073

Centisome position: 91.9

GC content: 67.51

Gene sequence:

>1542_bases
ATGGGCGGACTTCCCGCAATTCTCCTGCATTCTTCAAAGCCATTAAGGAGCAGAGGCTTGCGCAAAGGTCCGATGCGGAG
ACTTGTCCGGCTGGGATGGGTCATTCCAGTGCTGGTTGCGGCTTCACCGGCCACCGCATCGCAGGGCGTGAGCGCCGACC
TCGACCGCATCGCGCGCATCGACGACTCCGGGCCGAGGCTGAATGCCGTGGTCACGATCAACCCCGACGCGCGAAAGATG
GAGGAGGCGGCGCGCAAGGTTCGCGGCCCACTGGCGGGCCGGACCCTGCTGATAAAGGACAACATCGAAACGGCCGACCC
GATGGCGACGACCGCCGGCAGCCTTGCCCTGAAGGACAACGTCACCGGACGGGACGCCCCGCTGGTGGCCCGCCTGCGCA
GCGCGGGCGTCGTGATCCTGGGGAAGACGAACCTGTCCGAATGGGCCAACATCCGCTCCTCCGCCTCATCGAGCGGCTGG
AGCGCTGTCGGCGGGCAGACGAAGAACCCGCACGCACTTGACCGCAGCCCGTGCGGATCGTCCTCGGGAAGCGGAGCAGC
CGTGGCGGCGGGCCTCGCCTGGGGTGCCATCGGAACGGAAACGGACGGGTCGATCACCTGCCCGGCTTCGGTCAACGGCA
TCGTCGGCTTCAAGCCGACGGTGGGCATGGTCAGCCGCACCCACGTAGTTCCAATCAGCCACAGCCAGGACACCGCAGGG
CCGATGACACGAAGCGTGCGGGATGCCGCGCTGCTGATGAATGCACTGGTCGGCAGCGATCCGCAGGACGCCGCGACGGC
GGAGGCAGACCGGCGAAAGGTGGACTTTAGCGCCGGCCTGGAAACCGCGAGCCTGAACGGCGTGCGGATCGGCGTGCTGC
GCAAGCAGGCTGGCGCGCACCCGGGCGTCGTGACCCTGTTCGACAATGCACTGGCCGACCTTGCCCGGGCAGGCGCGCGG
CTGGTCGAGATCGACTACTCTCCGCCGCCGGAAATGGGACGCGACGAACTGACGGTATTGCTGTTCGAACTGCGCGAGGA
TCTGGGCGCATACTTGCGCTCGCTTCCGGGCGAGCCGCCCGTGCGCAGCCTTGCCGACGTAATCGCCTTCAACAAGGCGA
ATGGCGCCGAGTTGCGCTGGTTCGGGCAGGACACGTTCGAACAGGCCGAAGCGACGACCGACCGCGCTGCCTATGAAACG
GCACGCGCCAATTCGCTGCGCTTGGCGGGCGCGGAAGGGATCGACCGGTTGCTGAAGGAAAACGACGTCGCTTTCCTCGT
CGCGCCGACGGCTGGTCCTGCCTGGCCGATCGACCTCGTGACGGGAGACCACTTCCTGGACGTGGGCGCGGGCAGCCTTG
CCGCGATTGCCGGCTACCCTCACCTGTCCGTGCCGATGGGCGCGGTGGAAGGCCTGCCGGTGGGCCTTTCGATCATGGGG
GCGAAGTGGGACGACGCGCGGGTGCTGAAGGCAGGTGCGGCCTACGAACGGGCGCGCAGCGTTGCAGTACCCACGCCGCG
TCTCAAACGCTGGGGCGAATGA

Upstream 100 bases:

>100_bases
TAAGCGGCGGCAAGGATTTCGTCGGTTAACACGCAATCTGCGGCATGGCCGCGGCCCGCAACATCCCGCGACATGCCCCT
TGTTCGGGGGCCTCTTGCGG

Downstream 100 bases:

>100_bases
CCGGCGGAACCCGGGTGGCATCGGCGGCGAGCTGCGACTGGATGGCCATGTGCCGCGCGCTTGCCCGGGCGAGCATGGCC
CTTTCCGCCGGAGACTGACG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MGGLPAILLHSSKPLRSRGLRKGPMRRLVRLGWVIPVLVAASPATASQGVSADLDRIARIDDSGPRLNAVVTINPDARKM
EEAARKVRGPLAGRTLLIKDNIETADPMATTAGSLALKDNVTGRDAPLVARLRSAGVVILGKTNLSEWANIRSSASSSGW
SAVGGQTKNPHALDRSPCGSSSGSGAAVAAGLAWGAIGTETDGSITCPASVNGIVGFKPTVGMVSRTHVVPISHSQDTAG
PMTRSVRDAALLMNALVGSDPQDAATAEADRRKVDFSAGLETASLNGVRIGVLRKQAGAHPGVVTLFDNALADLARAGAR
LVEIDYSPPPEMGRDELTVLLFELREDLGAYLRSLPGEPPVRSLADVIAFNKANGAELRWFGQDTFEQAEATTDRAAYET
ARANSLRLAGAEGIDRLLKENDVAFLVAPTAGPAWPIDLVTGDHFLDVGAGSLAAIAGYPHLSVPMGAVEGLPVGLSIMG
AKWDDARVLKAGAAYERARSVAVPTPRLKRWGE

Sequences:

>Translated_513_residues
MGGLPAILLHSSKPLRSRGLRKGPMRRLVRLGWVIPVLVAASPATASQGVSADLDRIARIDDSGPRLNAVVTINPDARKM
EEAARKVRGPLAGRTLLIKDNIETADPMATTAGSLALKDNVTGRDAPLVARLRSAGVVILGKTNLSEWANIRSSASSSGW
SAVGGQTKNPHALDRSPCGSSSGSGAAVAAGLAWGAIGTETDGSITCPASVNGIVGFKPTVGMVSRTHVVPISHSQDTAG
PMTRSVRDAALLMNALVGSDPQDAATAEADRRKVDFSAGLETASLNGVRIGVLRKQAGAHPGVVTLFDNALADLARAGAR
LVEIDYSPPPEMGRDELTVLLFELREDLGAYLRSLPGEPPVRSLADVIAFNKANGAELRWFGQDTFEQAEATTDRAAYET
ARANSLRLAGAEGIDRLLKENDVAFLVAPTAGPAWPIDLVTGDHFLDVGAGSLAAIAGYPHLSVPMGAVEGLPVGLSIMG
AKWDDARVLKAGAAYERARSVAVPTPRLKRWGE
>Mature_512_residues
GGLPAILLHSSKPLRSRGLRKGPMRRLVRLGWVIPVLVAASPATASQGVSADLDRIARIDDSGPRLNAVVTINPDARKME
EAARKVRGPLAGRTLLIKDNIETADPMATTAGSLALKDNVTGRDAPLVARLRSAGVVILGKTNLSEWANIRSSASSSGWS
AVGGQTKNPHALDRSPCGSSSGSGAAVAAGLAWGAIGTETDGSITCPASVNGIVGFKPTVGMVSRTHVVPISHSQDTAGP
MTRSVRDAALLMNALVGSDPQDAATAEADRRKVDFSAGLETASLNGVRIGVLRKQAGAHPGVVTLFDNALADLARAGARL
VEIDYSPPPEMGRDELTVLLFELREDLGAYLRSLPGEPPVRSLADVIAFNKANGAELRWFGQDTFEQAEATTDRAAYETA
RANSLRLAGAEGIDRLLKENDVAFLVAPTAGPAWPIDLVTGDHFLDVGAGSLAAIAGYPHLSVPMGAVEGLPVGLSIMGA
KWDDARVLKAGAAYERARSVAVPTPRLKRWGE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=229, Percent_Identity=32.3144104803493, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI195972892, Length=220, Percent_Identity=30.4545454545455, Blast_Score=89, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=214, Percent_Identity=30.8411214953271, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=267, Percent_Identity=26.9662921348315, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=189, Percent_Identity=30.1587301587302, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=494, Percent_Identity=27.9352226720648, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323950, Length=223, Percent_Identity=33.1838565022422, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648113, Length=239, Percent_Identity=38.4937238493724, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=25.6862745098039, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=28.6290322580645, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=28.6290322580645, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=28.6290322580645, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648441, Length=177, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648439, Length=177, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356731, Length=477, Percent_Identity=26.6247379454927, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652985, Length=482, Percent_Identity=24.896265560166, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922090, Length=482, Percent_Identity=24.896265560166, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652981, Length=482, Percent_Identity=24.896265560166, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652983, Length=482, Percent_Identity=24.896265560166, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161078093, Length=435, Percent_Identity=25.5172413793103, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 53554; Mature: 53422

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLPAILLHSSKPLRSRGLRKGPMRRLVRLGWVIPVLVAASPATASQGVSADLDRIARI
CCCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
DDSGPRLNAVVTINPDARKMEEAARKVRGPLAGRTLLIKDNIETADPMATTAGSLALKDN
CCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHCCCCEEEECC
VTGRDAPLVARLRSAGVVILGKTNLSEWANIRSSASSSGWSAVGGQTKNPHALDRSPCGS
CCCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SSGSGAAVAAGLAWGAIGTETDGSITCPASVNGIVGFKPTVGMVSRTHVVPISHSQDTAG
CCCCCCEEEHHHHHCCCCCCCCCCEECCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCC
PMTRSVRDAALLMNALVGSDPQDAATAEADRRKVDFSAGLETASLNGVRIGVLRKQAGAH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEHHCCCCEECCCCEEEEEEEHHCCCC
PGVVTLFDNALADLARAGARLVEIDYSPPPEMGRDELTVLLFELREDLGAYLRSLPGEPP
CCEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VRSLADVIAFNKANGAELRWFGQDTFEQAEATTDRAAYETARANSLRLAGAEGIDRLLKE
HHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHC
NDVAFLVAPTAGPAWPIDLVTGDHFLDVGAGSLAAIAGYPHLSVPMGAVEGLPVGLSIMG
CCEEEEEECCCCCCCEEEEECCCCEEECCCCCEEEEECCCCCCCCCCHHCCCCCCEEEEC
AKWDDARVLKAGAAYERARSVAVPTPRLKRWGE
CCCCCCHHHHHCHHHHHHCCCCCCCHHHHCCCC
>Mature Secondary Structure 
GGLPAILLHSSKPLRSRGLRKGPMRRLVRLGWVIPVLVAASPATASQGVSADLDRIARI
CCCCEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
DDSGPRLNAVVTINPDARKMEEAARKVRGPLAGRTLLIKDNIETADPMATTAGSLALKDN
CCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHCCCCEEEECC
VTGRDAPLVARLRSAGVVILGKTNLSEWANIRSSASSSGWSAVGGQTKNPHALDRSPCGS
CCCCCCHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
SSGSGAAVAAGLAWGAIGTETDGSITCPASVNGIVGFKPTVGMVSRTHVVPISHSQDTAG
CCCCCCEEEHHHHHCCCCCCCCCCEECCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCC
PMTRSVRDAALLMNALVGSDPQDAATAEADRRKVDFSAGLETASLNGVRIGVLRKQAGAH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEHHCCCCEECCCCEEEEEEEHHCCCC
PGVVTLFDNALADLARAGARLVEIDYSPPPEMGRDELTVLLFELREDLGAYLRSLPGEPP
CCEEEHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VRSLADVIAFNKANGAELRWFGQDTFEQAEATTDRAAYETARANSLRLAGAEGIDRLLKE
HHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHC
NDVAFLVAPTAGPAWPIDLVTGDHFLDVGAGSLAAIAGYPHLSVPMGAVEGLPVGLSIMG
CCEEEEEECCCCCCCEEEEECCCCEEECCCCCEEEEECCCCCCCCCCHHCCCCCCEEEEC
AKWDDARVLKAGAAYERARSVAVPTPRLKRWGE
CCCCCCHHHHHCHHHHHHCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]