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The map label for this gene is gloA [H]

Identifier: 87201049

GI number: 87201049

Start: 3252293

End: 3252730

Strand: Reverse

Name: gloA [H]

Synonym: Saro_3037

Alternate gene names: 87201049

Gene position: 3252730-3252293 (Counterclockwise)

Preceding gene: 87201051

Following gene: 87201048

Centisome position: 91.33

GC content: 63.24

Gene sequence:

>438_bases
GTGACCAAGTTCCTGCATACCATGATTCGCGTGACCGATCCCGACGCGACTATCGCGTTCTTCGAACTGATCGGCCTGAA
GGAGACGCGGCGCTTCGACAGCGAGAAGGGCCGCTTCACCCTGATCTTCCTGGCGGCTCCCGGCGAGGAGGGCATCGCCG
AGGTCGAGCTGACCTACAACTGGCCGCCCGAGGACGGTTCGCCCGCAGAGGGGTATTCGGGCGGGCGCAATTTCGGGCAC
CTCGCGTACCGCGTCGACAATATCTATGATACCTGCCGGCGCATCATGGATGCAGGCCATATCGTCCACCGTCCTCCGCG
CGACGGACACATGGCCTTCGTGAAGACGCCGGACGGAATTTCGATCGAACTGCTGCAGGACGGTCACCTGCCGCCGCAGG
AGCCGTGGGCGAGCATGGAGAACACCGGAACCTGGTAA

Upstream 100 bases:

>100_bases
CTATCCTCTGCCGGCATCGTCATCTCCTTGCGGGCGTGGCCTGCGATGAAATGTGCGATTTCATCGATGAACCGTTTTTG
AACGAGAAGCGAGCTTCCCC

Downstream 100 bases:

>100_bases
GCCGCGCGCGGCTGAACCAGAGGAACGCCACCGGCGTCGGACCAAGCGGTCCGGCGCCGGTTTTTTCGTGCCGGCGGTGC
ACCTGCGGAAAAGGGTGGAG

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MTKFLHTMIRVTDPDATIAFFELIGLKETRRFDSEKGRFTLIFLAAPGEEGIAEVELTYNWPPEDGSPAEGYSGGRNFGH
LAYRVDNIYDTCRRIMDAGHIVHRPPRDGHMAFVKTPDGISIELLQDGHLPPQEPWASMENTGTW

Sequences:

>Translated_145_residues
MTKFLHTMIRVTDPDATIAFFELIGLKETRRFDSEKGRFTLIFLAAPGEEGIAEVELTYNWPPEDGSPAEGYSGGRNFGH
LAYRVDNIYDTCRRIMDAGHIVHRPPRDGHMAFVKTPDGISIELLQDGHLPPQEPWASMENTGTW
>Mature_144_residues
TKFLHTMIRVTDPDATIAFFELIGLKETRRFDSEKGRFTLIFLAAPGEEGIAEVELTYNWPPEDGSPAEGYSGGRNFGHL
AYRVDNIYDTCRRIMDAGHIVHRPPRDGHMAFVKTPDGISIELLQDGHLPPQEPWASMENTGTW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=143, Percent_Identity=32.8671328671329, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1787940, Length=131, Percent_Identity=35.1145038167939, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323639, Length=144, Percent_Identity=31.9444444444444, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=30.1369863013699, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16361; Mature: 16229

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFLHTMIRVTDPDATIAFFELIGLKETRRFDSEKGRFTLIFLAAPGEEGIAEVELTYN
CCHHHEEHEEEECCCCCEEEHHHHCCHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEE
WPPEDGSPAEGYSGGRNFGHLAYRVDNIYDTCRRIMDAGHIVHRPPRDGHMAFVKTPDGI
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCC
SIELLQDGHLPPQEPWASMENTGTW
EEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKFLHTMIRVTDPDATIAFFELIGLKETRRFDSEKGRFTLIFLAAPGEEGIAEVELTYN
CHHHEEHEEEECCCCCEEEHHHHCCHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEE
WPPEDGSPAEGYSGGRNFGHLAYRVDNIYDTCRRIMDAGHIVHRPPRDGHMAFVKTPDGI
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCC
SIELLQDGHLPPQEPWASMENTGTW
EEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]