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The map label for this gene is dmlA [H]

Identifier: 87200947

GI number: 87200947

Start: 3150843

End: 3151922

Strand: Reverse

Name: dmlA [H]

Synonym: Saro_2935

Alternate gene names: 87200947

Gene position: 3151922-3150843 (Counterclockwise)

Preceding gene: 87200948

Following gene: 87200946

Centisome position: 88.5

GC content: 65.0

Gene sequence:

>1080_bases
ATGAGCAGCAACACCCGCAGCTATTCGATCGCGGTCATCCCCGGGGACGGCATCGGCAAGGAAGTCATGCCCGAAGGCAT
GCGCGTGCTGGAAAAGGCCGCATCGATCTTCGGTTTCGAGATCGAACAGAAGTGGCACGACTTTGCGTGCTGCGATTACT
ACGCGCGCCACGGCAAGATGATGCCCGACAACTGGAAGGAAGAGATCGGCCGCCCCGACGCGATCTTCTTCGGCGCGGTC
GGCTGGCCGGATACCGTGCCGGATCACGTGTCGCTGTGGGGTTCGCTTCTCCAGTTTCGCCGCGAGTATGACCAGTACGT
CAACCTGCGCCCCGTCCGCCTGATGCCCGGCGTGCCGTGCCCCCTGGCCGGGCGCGAGCCCGGCGACATCGACTTCTGGG
TGGTGCGCGAAAACACCGAGGGCGAATATTCCTCGGTCGGCGGCATCATGTTTCCCAATACCGACCGCGAAGTCGTGATC
CAGGAAACGGTGATGACCCGCATCGGCGTCGACCGCGTGCTGCGTTATGCCTTCGACCTCGCGCAGAAGCGCGAGCGCAA
GCACCTGACCAGCGCGACCAAGTCCAACGGCATCGCTATCACCATGCCCTATTGGGACCAGCGCGTCGAAGCCATCGGCG
CCGAGTATCCCGACGTCAGCCACGACAAGTACCACATCGACATCCTGACCGCGCAGTTCGTGCTGAACCCCGACCGCTTC
GACGTGGTCGTCGGCTCCAACCTGTTCGGCGATATCCTCTCCGACCTCGGCCCGGCCTGCACCGGGACGATCGGCGTTGC
ACCTTCGGGCAACATCAATCCCGAAGGCATCCACCCCTCGCTGTTCGAGCCGGTCCACGGTTCTGCCCCCGACATCGCGG
GCCAGGGCATCGCCAATCCCGTCGGCCAGATCTGGTCGGCGGCGATGATGCTGGAACACCTGGGCGAGGCAGAGGCCGCC
GCTGCCATCATGCGCGCGGTCGAGCAGGTCCTCGCTACGCCATCGGCCCGCACTCGCGATCTCAAGGGCGCTGCAAGCAC
CGATGCCTGCGGCAAGGCCATCGCCGAAGCGCTCGGCTAG

Upstream 100 bases:

>100_bases
GGCCTCGGCCGCGAGGGTGGCCTTGCCGGCATCGAGGAATACCTTGAAACCAAGGCCTTCCACATCGGCGGCCTTAAGAT
CTGACCACTTGGGGGGAACC

Downstream 100 bases:

>100_bases
GCCATTGCGGAGCGCGGCAGAAAATGCTGCGCTCCGCCGTTCGAAAGCGCCGGGGGCGCGCGTTCATCCCCGCATTCGGC
CAAACCCTCTCTTGTCGAGG

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSSNTRSYSIAVIPGDGIGKEVMPEGMRVLEKAASIFGFEIEQKWHDFACCDYYARHGKMMPDNWKEEIGRPDAIFFGAV
GWPDTVPDHVSLWGSLLQFRREYDQYVNLRPVRLMPGVPCPLAGREPGDIDFWVVRENTEGEYSSVGGIMFPNTDREVVI
QETVMTRIGVDRVLRYAFDLAQKRERKHLTSATKSNGIAITMPYWDQRVEAIGAEYPDVSHDKYHIDILTAQFVLNPDRF
DVVVGSNLFGDILSDLGPACTGTIGVAPSGNINPEGIHPSLFEPVHGSAPDIAGQGIANPVGQIWSAAMMLEHLGEAEAA
AAIMRAVEQVLATPSARTRDLKGAASTDACGKAIAEALG

Sequences:

>Translated_359_residues
MSSNTRSYSIAVIPGDGIGKEVMPEGMRVLEKAASIFGFEIEQKWHDFACCDYYARHGKMMPDNWKEEIGRPDAIFFGAV
GWPDTVPDHVSLWGSLLQFRREYDQYVNLRPVRLMPGVPCPLAGREPGDIDFWVVRENTEGEYSSVGGIMFPNTDREVVI
QETVMTRIGVDRVLRYAFDLAQKRERKHLTSATKSNGIAITMPYWDQRVEAIGAEYPDVSHDKYHIDILTAQFVLNPDRF
DVVVGSNLFGDILSDLGPACTGTIGVAPSGNINPEGIHPSLFEPVHGSAPDIAGQGIANPVGQIWSAAMMLEHLGEAEAA
AAIMRAVEQVLATPSARTRDLKGAASTDACGKAIAEALG
>Mature_358_residues
SSNTRSYSIAVIPGDGIGKEVMPEGMRVLEKAASIFGFEIEQKWHDFACCDYYARHGKMMPDNWKEEIGRPDAIFFGAVG
WPDTVPDHVSLWGSLLQFRREYDQYVNLRPVRLMPGVPCPLAGREPGDIDFWVVRENTEGEYSSVGGIMFPNTDREVVIQ
ETVMTRIGVDRVLRYAFDLAQKRERKHLTSATKSNGIAITMPYWDQRVEAIGAEYPDVSHDKYHIDILTAQFVLNPDRFD
VVVGSNLFGDILSDLGPACTGTIGVAPSGNINPEGIHPSLFEPVHGSAPDIAGQGIANPVGQIWSAAMMLEHLGEAEAAA
AIMRAVEQVLATPSARTRDLKGAASTDACGKAIAEALG

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=338, Percent_Identity=31.9526627218935, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI28178821, Length=350, Percent_Identity=28, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI28178816, Length=354, Percent_Identity=27.9661016949153, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4758582, Length=342, Percent_Identity=28.9473684210526, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28178838, Length=321, Percent_Identity=29.595015576324, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI28178819, Length=201, Percent_Identity=31.3432835820896, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=65.6424581005587, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=39.3939393939394, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787381, Length=368, Percent_Identity=29.6195652173913, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71986051, Length=349, Percent_Identity=32.378223495702, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17550882, Length=344, Percent_Identity=29.6511627906977, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=27.0949720670391, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=340, Percent_Identity=26.7647058823529, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322097, Length=364, Percent_Identity=36.5384615384615, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=359, Percent_Identity=32.3119777158774, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324291, Length=355, Percent_Identity=32.9577464788732, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=32.5068870523416, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24643270, Length=359, Percent_Identity=32.033426183844, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24643268, Length=359, Percent_Identity=32.033426183844, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24661184, Length=356, Percent_Identity=31.7415730337079, Blast_Score=155, Evalue=6e-38,
Organism=Drosophila melanogaster, GI161078633, Length=353, Percent_Identity=28.8951841359773, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24650122, Length=353, Percent_Identity=28.8951841359773, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI161078637, Length=353, Percent_Identity=28.8951841359773, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI161078635, Length=353, Percent_Identity=28.8951841359773, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI161078639, Length=263, Percent_Identity=32.6996197718631, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=27.2727272727273, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=27.2727272727273, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI20130355, Length=360, Percent_Identity=25, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 39250; Mature: 39119

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNTRSYSIAVIPGDGIGKEVMPEGMRVLEKAASIFGFEIEQKWHDFACCDYYARHGKM
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCC
MPDNWKEEIGRPDAIFFGAVGWPDTVPDHVSLWGSLLQFRREYDQYVNLRPVRLMPGVPC
CCCCHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PLAGREPGDIDFWVVRENTEGEYSSVGGIMFPNTDREVVIQETVMTRIGVDRVLRYAFDL
CCCCCCCCCEEEEEEEECCCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQKRERKHLTSATKSNGIAITMPYWDQRVEAIGAEYPDVSHDKYHIDILTAQFVLNPDRF
HHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCE
DVVVGSNLFGDILSDLGPACTGTIGVAPSGNINPEGIHPSLFEPVHGSAPDIAGQGIANP
EEEECCHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCH
VGQIWSAAMMLEHLGEAEAAAAIMRAVEQVLATPSARTRDLKGAASTDACGKAIAEALG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
SSNTRSYSIAVIPGDGIGKEVMPEGMRVLEKAASIFGFEIEQKWHDFACCDYYARHGKM
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCC
MPDNWKEEIGRPDAIFFGAVGWPDTVPDHVSLWGSLLQFRREYDQYVNLRPVRLMPGVPC
CCCCHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
PLAGREPGDIDFWVVRENTEGEYSSVGGIMFPNTDREVVIQETVMTRIGVDRVLRYAFDL
CCCCCCCCCEEEEEEEECCCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQKRERKHLTSATKSNGIAITMPYWDQRVEAIGAEYPDVSHDKYHIDILTAQFVLNPDRF
HHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCE
DVVVGSNLFGDILSDLGPACTGTIGVAPSGNINPEGIHPSLFEPVHGSAPDIAGQGIANP
EEEECCHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCH
VGQIWSAAMMLEHLGEAEAAAAIMRAVEQVLATPSARTRDLKGAASTDACGKAIAEALG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]