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The map label for this gene is hemC

Identifier: 87200799

GI number: 87200799

Start: 3006518

End: 3007456

Strand: Direct

Name: hemC

Synonym: Saro_2786

Alternate gene names: 87200799

Gene position: 3006518-3007456 (Clockwise)

Preceding gene: 87200794

Following gene: 87200800

Centisome position: 84.42

GC content: 70.07

Gene sequence:

>939_bases
ATGAACAACCAAGCTCCGCAAAGGCCCCTGAAGCTGGGCACCCGCCGCTCTCCCCTTGCCATGGCACAGGCCGAGGAAGC
CCGTGCCCGCCTCTGCGCGGCGCATGGCTGGGCCGAATCGCAGGTGGAACTTGTCCCGGTGACGGCCAGCGGCGACAAGA
TCCAGGACCGTCCGCTCGCCGACATCGGCGGCAAGGCGCTCTGGACCAAGGAGCTCGACGCCTGGCTCGTCTCGGGCGAG
ATCGACTTTGCCGTGCATTCGATGAAGGACGTCGAGACGATCCGTCCTGAAAACTTCGCCATCGCCGCCGTCCTTCCCCG
CGCCGACGTGCGCGACGTGCTGATCGGCGCGGCCTCCATCCACGCAATCCCGCCCGGTGCGCGCGTCGGCACCAGCGCCC
CGCGCCGCGCCGCGCAGATGCTCCACGCCCGGCCCGACCTCACTGTGGTTTCGTTCCGGGGCAATGTCGCGACCCGTCTG
GCAAAGCTCCAGGCGGGCGAGGCCGACGTGACCCTGCTTGCCGCCGCCGGCCTCGGACGCCTGGGCGAGACCGGGGTCGG
CCATCCGCTTTCGGAGTACGAGTGGCTCCCCGCGCCTGCCCAGGGCGCGATCGGAATCGAATGCCTCGCGTCGAGAGCCG
ATCTCTGCGCATGGCTCGGCGCCATCGACGACAAGCCGACCCATCACGCGATCCGCGCCGAACGCGCCCTGCTCCTCGCG
CTCGGCGGCAATTGCCACAGCCCGATCGCGGTCCTGACCACGCATCACGAAAGCTCGCTGACGCTTCGCGCCGCGTTGTT
CAGCCCGGACGGTGCCGAGCGCGTCGAAGCGCACGAGACCTTCGCGGCGGGCGACGATGCAGGCCCCGCCCATCTCGCCC
ATCGCCTCCTAGGCGAAGCGCCCGACGCGATCCGCGTGCATTTCCACGGAGCCGGATGA

Upstream 100 bases:

>100_bases
TCACCGCGGCGGCGGTTTCATCGCAGCTGGATTCTATGCCAAGGATCAGGGCCATGTGGCGCTTTCCTCTAGAGATAATC
CCGTTTACGGCAAGCGGCGC

Downstream 100 bases:

>100_bases
AGCTCCTGCCGCTCATCGTCATCCGTCCCGAGCCCGGCAATGCCGCAACCATTGCCGCCGCGCGCGAACGGGGCCTTCAT
GCGATCGGCTACCCGCTCTT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNNQAPQRPLKLGTRRSPLAMAQAEEARARLCAAHGWAESQVELVPVTASGDKIQDRPLADIGGKALWTKELDAWLVSGE
IDFAVHSMKDVETIRPENFAIAAVLPRADVRDVLIGAASIHAIPPGARVGTSAPRRAAQMLHARPDLTVVSFRGNVATRL
AKLQAGEADVTLLAAAGLGRLGETGVGHPLSEYEWLPAPAQGAIGIECLASRADLCAWLGAIDDKPTHHAIRAERALLLA
LGGNCHSPIAVLTTHHESSLTLRAALFSPDGAERVEAHETFAAGDDAGPAHLAHRLLGEAPDAIRVHFHGAG

Sequences:

>Translated_312_residues
MNNQAPQRPLKLGTRRSPLAMAQAEEARARLCAAHGWAESQVELVPVTASGDKIQDRPLADIGGKALWTKELDAWLVSGE
IDFAVHSMKDVETIRPENFAIAAVLPRADVRDVLIGAASIHAIPPGARVGTSAPRRAAQMLHARPDLTVVSFRGNVATRL
AKLQAGEADVTLLAAAGLGRLGETGVGHPLSEYEWLPAPAQGAIGIECLASRADLCAWLGAIDDKPTHHAIRAERALLLA
LGGNCHSPIAVLTTHHESSLTLRAALFSPDGAERVEAHETFAAGDDAGPAHLAHRLLGEAPDAIRVHFHGAG
>Mature_312_residues
MNNQAPQRPLKLGTRRSPLAMAQAEEARARLCAAHGWAESQVELVPVTASGDKIQDRPLADIGGKALWTKELDAWLVSGE
IDFAVHSMKDVETIRPENFAIAAVLPRADVRDVLIGAASIHAIPPGARVGTSAPRRAAQMLHARPDLTVVSFRGNVATRL
AKLQAGEADVTLLAAAGLGRLGETGVGHPLSEYEWLPAPAQGAIGIECLASRADLCAWLGAIDDKPTHHAIRAERALLLA
LGGNCHSPIAVLTTHHESSLTLRAALFSPDGAERVEAHETFAAGDDAGPAHLAHRLLGEAPDAIRVHFHGAG

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps [H]

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family [H]

Homologues:

Organism=Homo sapiens, GI20149500, Length=288, Percent_Identity=37.1527777777778, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI66933009, Length=283, Percent_Identity=37.4558303886926, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI48994974, Length=280, Percent_Identity=41.0714285714286, Blast_Score=184, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6319996, Length=281, Percent_Identity=34.5195729537367, Blast_Score=145, Evalue=9e-36,
Organism=Drosophila melanogaster, GI20130425, Length=291, Percent_Identity=35.7388316151203, Blast_Score=160, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418 [H]

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC [H]

EC number: =2.5.1.61 [H]

Molecular weight: Translated: 32886; Mature: 32886

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00436 PEROXIDASE_2 ; PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQAPQRPLKLGTRRSPLAMAQAEEARARLCAAHGWAESQVELVPVTASGDKIQDRPLA
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCH
DIGGKALWTKELDAWLVSGEIDFAVHSMKDVETIRPENFAIAAVLPRADVRDVLIGAASI
HCCCCEEEEECCCEEEECCCEEEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHE
HAIPPGARVGTSAPRRAAQMLHARPDLTVVSFRGNVATRLAKLQAGEADVTLLAAAGLGR
EECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCEEEEEECCCCC
LGETGVGHPLSEYEWLPAPAQGAIGIECLASRADLCAWLGAIDDKPTHHAIRAERALLLA
CCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEEEE
LGGNCHSPIAVLTTHHESSLTLRAALFSPDGAERVEAHETFAAGDDAGPAHLAHRLLGEA
ECCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
PDAIRVHFHGAG
CCEEEEEEECCC
>Mature Secondary Structure
MNNQAPQRPLKLGTRRSPLAMAQAEEARARLCAAHGWAESQVELVPVTASGDKIQDRPLA
CCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCH
DIGGKALWTKELDAWLVSGEIDFAVHSMKDVETIRPENFAIAAVLPRADVRDVLIGAASI
HCCCCEEEEECCCEEEECCCEEEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHE
HAIPPGARVGTSAPRRAAQMLHARPDLTVVSFRGNVATRLAKLQAGEADVTLLAAAGLGR
EECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCEEEEEECCCCC
LGETGVGHPLSEYEWLPAPAQGAIGIECLASRADLCAWLGAIDDKPTHHAIRAERALLLA
CCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCEEEEE
LGGNCHSPIAVLTTHHESSLTLRAALFSPDGAERVEAHETFAAGDDAGPAHLAHRLLGEA
ECCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCC
PDAIRVHFHGAG
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA