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The map label for this gene is epsD [H]

Identifier: 87200666

GI number: 87200666

Start: 2889499

End: 2891745

Strand: Reverse

Name: epsD [H]

Synonym: Saro_2653

Alternate gene names: 87200666

Gene position: 2891745-2889499 (Counterclockwise)

Preceding gene: 87200667

Following gene: 87200665

Centisome position: 81.19

GC content: 66.09

Gene sequence:

>2247_bases
ATGAAACTGCGCACGCTTTTCGGCCCCCGGATGCTTCTCGGCGCCGCCGCACTTGCCCTTGCTGTCCCACAGGCGGCTCT
GGCCCAATACACCCTCAACGTGCGCGAGGCCGACATTCGCGCCTTCGTTGCCGATGCCGCAGAGGTCACCGGGCGCACGT
TCATCGTCGACAGCCGCGTGCAGGCAAAGATCAGCGTGGTCTCGGACCGGCCGTTGAGCCGCTCGGAATACTTCGAGGTC
TTCCTTTCCACCTTGCGCGCCAACGGCCTCGTTGCCGTGCCGACCGGCAATGGCGCATTCCGCATCCAGCCCGCGGAAGG
CGCGGCCACCAATCCCACTCGCATCGGCAGCCGAGGCGCGGCGCAGAGCCAGATGGTGACCGAGGTCGTCCGACTGCGGA
CCATCGATGCCGCCCAGGCGGTCGAAACCCTGCGCCCGCTCGTCAGCAAGGAAGGCGCGATCACCGCGAACAAGGCGGGC
AATTCGCTGGTCATCGTCGACTATGCCGACAATCTGCGCCGCATTCGTGCGCTGCTCGGCGATATCGACCGCGACAGCAC
GACGACCCAGACCGTCATCCTCGAACATGCCGGTGCGCGCGAAATCGCCACTGCGCTTACCCAGCTCGTCCCCGCAGGTG
CCGAAGGCGCGCGGGCGCTGGCAACTGTCGTTGCCGTCGACAGCGCGAACGCGGTGCTGATGCGCGGCGATGGCTCGACG
TTGTCGCGCCTCTCCGCGATGGCTCGGCAGCTTGATGCCAAGGCCGCGCTGGGTGGCGAAATCAAGGTCGTCTGGCTCGA
TTATGCCGATTCGGCCGCGCTCGTGCCGGTGCTGGAACGGCTGATCGGCGGGCAGGGCGGCGGCGAAGTGGCTTCGGCCA
GCGCCGCGCCCATCTCGCTCGGCGGGGTTGGCAGCAACGCGACCTCGACGGCGGGAACGCAGGGCAGCACCGGCCAGGCC
GCGTCGACCGGCGGCTCCTCGACCAGCCCGATCGGCGCTACCTCGGGCGGGCTCGGCAATGGGCCGATCAAGCTGGCGAA
CGGACGCGGGACCGCCACGATCACGCGTTATCCGGGTGCCAACGCGATCATCATCGCCGCCCCCGCCGACGAGCAGCGTC
GCCTCAGCGAACTGATCCGCCAGCTCGACCTTCCGCAGGAACAGGTTCTGGTAGAGGCGATCATCGTCGAGATCTCGAAC
GACGTGGCGAAGGAATTGGGCGTCCAGATGCTGTTCGGCGGCAAGAACATGCCCTTTGCCGTCACCAACTATTCCAACAG
TTCGCCCAACATCATCGACATCGCGGGTGGGCTGCTGGCTGACAACCTCAACCAGACCACGACCGTGGTGAATGGCGATA
CCGTCACCACCACGACCAACAGCACGGCGGGCGATCTCCTGCGCGAGAATGCGGCAAGCAAGCTCCTGTCCGCGCGCGGG
GCCTATACCGGTTTCCTCACCGAGCTGTCCAAGGACACTTATCTCGGCGCGATCATCAACGCGGTGCAGACCGACAAGAA
CTCGAACATCCTGTCGACGCCGCACATCACCACCAACAACAACGTTCCCGCCTCGATCCTGTTCGGGCAGGAAATCCCTG
TGTCGACCGGCGAGGCCCTCTCGGGCGGCAATTTCAGCGACACTTTCCGCACGATCCAGCGCCAGAACGTCGGCATCGAA
CTGGACGTGACGCCGCAGATCAATGCCAGCAACATGATCCGGCTGGACCTGCGCCAGGAAGTCAGCTCCATCGCCGGGAC
CGTGTCGAAGACCTCGAACGAACTCATCGTCAACAAGCGCGAGATCAAGACCACCGTCACCGTGGGCGACCGCGAGATCG
TCGCCCTCGGCGGCCTGCTCGACGACAACGAGCAGCGCACCTTGCAGAAGGTGCCGCTCCTCGGCGACATTCCCATCCTG
GGCGAGGCGTTCAAGTCGCGCGCGCGCAGCCGGGTGAAGACCAACCTCATGGTGTTCATCCGCCCGACCATCCTGCGCAA
TGCCGCCGACCGCCAGGCTCTTGCCGCGCGGCGCTATGGCGTGGTGCGCGATGCGCAGGTCAAGTTCGATTCCAAGCGTG
AACCGACCATCGACGATCTGATCGTCGACTACATGGGCGCCACCTTGCCGGTCGCTCCTTCCGCCGAGGTCGCGGTGGTA
GCGCCGGGCGATACGGTCGTGCGCCCAAGGGCTGCCGCGACGCCGCTCGAACCCGCCGAACTCCCGCCCAGCAGCGCGCG
GAACTGA

Upstream 100 bases:

>100_bases
ATGCTGCAATGCTTGCCGGCGCACTGCGTCCCGGCGCTTCGATCTCGGTCACCGTCAGGCGCGGCGACCGGCAACTTCCT
CTCGCGATCACATTGGCACC

Downstream 100 bases:

>100_bases
CATCGTGGAGGCGGGCGAAGACATCGAGGGCATGACCGTTGCGGTCGCGCAGGCCGGGCCTGCGATGCCCTATGCCTTCG
CGCGCGACCGCGGCGTCCTC

Product: type II and III secretion system protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MKLRTLFGPRMLLGAAALALAVPQAALAQYTLNVREADIRAFVADAAEVTGRTFIVDSRVQAKISVVSDRPLSRSEYFEV
FLSTLRANGLVAVPTGNGAFRIQPAEGAATNPTRIGSRGAAQSQMVTEVVRLRTIDAAQAVETLRPLVSKEGAITANKAG
NSLVIVDYADNLRRIRALLGDIDRDSTTTQTVILEHAGAREIATALTQLVPAGAEGARALATVVAVDSANAVLMRGDGST
LSRLSAMARQLDAKAALGGEIKVVWLDYADSAALVPVLERLIGGQGGGEVASASAAPISLGGVGSNATSTAGTQGSTGQA
ASTGGSSTSPIGATSGGLGNGPIKLANGRGTATITRYPGANAIIIAAPADEQRRLSELIRQLDLPQEQVLVEAIIVEISN
DVAKELGVQMLFGGKNMPFAVTNYSNSSPNIIDIAGGLLADNLNQTTTVVNGDTVTTTTNSTAGDLLRENAASKLLSARG
AYTGFLTELSKDTYLGAIINAVQTDKNSNILSTPHITTNNNVPASILFGQEIPVSTGEALSGGNFSDTFRTIQRQNVGIE
LDVTPQINASNMIRLDLRQEVSSIAGTVSKTSNELIVNKREIKTTVTVGDREIVALGGLLDDNEQRTLQKVPLLGDIPIL
GEAFKSRARSRVKTNLMVFIRPTILRNAADRQALAARRYGVVRDAQVKFDSKREPTIDDLIVDYMGATLPVAPSAEVAVV
APGDTVVRPRAAATPLEPAELPPSSARN

Sequences:

>Translated_748_residues
MKLRTLFGPRMLLGAAALALAVPQAALAQYTLNVREADIRAFVADAAEVTGRTFIVDSRVQAKISVVSDRPLSRSEYFEV
FLSTLRANGLVAVPTGNGAFRIQPAEGAATNPTRIGSRGAAQSQMVTEVVRLRTIDAAQAVETLRPLVSKEGAITANKAG
NSLVIVDYADNLRRIRALLGDIDRDSTTTQTVILEHAGAREIATALTQLVPAGAEGARALATVVAVDSANAVLMRGDGST
LSRLSAMARQLDAKAALGGEIKVVWLDYADSAALVPVLERLIGGQGGGEVASASAAPISLGGVGSNATSTAGTQGSTGQA
ASTGGSSTSPIGATSGGLGNGPIKLANGRGTATITRYPGANAIIIAAPADEQRRLSELIRQLDLPQEQVLVEAIIVEISN
DVAKELGVQMLFGGKNMPFAVTNYSNSSPNIIDIAGGLLADNLNQTTTVVNGDTVTTTTNSTAGDLLRENAASKLLSARG
AYTGFLTELSKDTYLGAIINAVQTDKNSNILSTPHITTNNNVPASILFGQEIPVSTGEALSGGNFSDTFRTIQRQNVGIE
LDVTPQINASNMIRLDLRQEVSSIAGTVSKTSNELIVNKREIKTTVTVGDREIVALGGLLDDNEQRTLQKVPLLGDIPIL
GEAFKSRARSRVKTNLMVFIRPTILRNAADRQALAARRYGVVRDAQVKFDSKREPTIDDLIVDYMGATLPVAPSAEVAVV
APGDTVVRPRAAATPLEPAELPPSSARN
>Mature_748_residues
MKLRTLFGPRMLLGAAALALAVPQAALAQYTLNVREADIRAFVADAAEVTGRTFIVDSRVQAKISVVSDRPLSRSEYFEV
FLSTLRANGLVAVPTGNGAFRIQPAEGAATNPTRIGSRGAAQSQMVTEVVRLRTIDAAQAVETLRPLVSKEGAITANKAG
NSLVIVDYADNLRRIRALLGDIDRDSTTTQTVILEHAGAREIATALTQLVPAGAEGARALATVVAVDSANAVLMRGDGST
LSRLSAMARQLDAKAALGGEIKVVWLDYADSAALVPVLERLIGGQGGGEVASASAAPISLGGVGSNATSTAGTQGSTGQA
ASTGGSSTSPIGATSGGLGNGPIKLANGRGTATITRYPGANAIIIAAPADEQRRLSELIRQLDLPQEQVLVEAIIVEISN
DVAKELGVQMLFGGKNMPFAVTNYSNSSPNIIDIAGGLLADNLNQTTTVVNGDTVTTTTNSTAGDLLRENAASKLLSARG
AYTGFLTELSKDTYLGAIINAVQTDKNSNILSTPHITTNNNVPASILFGQEIPVSTGEALSGGNFSDTFRTIQRQNVGIE
LDVTPQINASNMIRLDLRQEVSSIAGTVSKTSNELIVNKREIKTTVTVGDREIVALGGLLDDNEQRTLQKVPLLGDIPIL
GEAFKSRARSRVKTNLMVFIRPTILRNAADRQALAARRYGVVRDAQVKFDSKREPTIDDLIVDYMGATLPVAPSAEVAVV
APGDTVVRPRAAATPLEPAELPPSSARN

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=688, Percent_Identity=29.796511627907, Blast_Score=245, Evalue=8e-66,
Organism=Escherichia coli, GI1789793, Length=336, Percent_Identity=26.4880952380952, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 78218; Mature: 78218

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRTLFGPRMLLGAAALALAVPQAALAQYTLNVREADIRAFVADAAEVTGRTFIVDSRV
CCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCC
QAKISVVSDRPLSRSEYFEVFLSTLRANGLVAVPTGNGAFRIQPAEGAATNPTRIGSRGA
EEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
AQSQMVTEVVRLRTIDAAQAVETLRPLVSKEGAITANKAGNSLVIVDYADNLRRIRALLG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECHHHHHHHHHHHC
DIDRDSTTTQTVILEHAGAREIATALTQLVPAGAEGARALATVVAVDSANAVLMRGDGST
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCEEEEECCCHH
LSRLSAMARQLDAKAALGGEIKVVWLDYADSAALVPVLERLIGGQGGGEVASASAAPISL
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEE
GGVGSNATSTAGTQGSTGQAASTGGSSTSPIGATSGGLGNGPIKLANGRGTATITRYPGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCC
NAIIIAAPADEQRRLSELIRQLDLPQEQVLVEAIIVEISNDVAKELGVQMLFGGKNMPFA
CEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEE
VTNYSNSSPNIIDIAGGLLADNLNQTTTVVNGDTVTTTTNSTAGDLLRENAASKLLSARG
EEECCCCCCCEEEECCCHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCC
AYTGFLTELSKDTYLGAIINAVQTDKNSNILSTPHITTNNNVPASILFGQEIPVSTGEAL
CHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCEEECCCCCCEEEEECCCCCCCCCCCC
SGGNFSDTFRTIQRQNVGIELDVTPQINASNMIRLDLRQEVSSIAGTVSKTSNELIVNKR
CCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEHH
EIKTTVTVGDREIVALGGLLDDNEQRTLQKVPLLGDIPILGEAFKSRARSRVKTNLMVFI
HEEEEEEECCCEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEE
RPTILRNAADRQALAARRYGVVRDAQVKFDSKREPTIDDLIVDYMGATLPVAPSAEVAVV
EHHHHHCCHHHHHHHHHHHCCEECCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEEE
APGDTVVRPRAAATPLEPAELPPSSARN
CCCCCEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLRTLFGPRMLLGAAALALAVPQAALAQYTLNVREADIRAFVADAAEVTGRTFIVDSRV
CCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCC
QAKISVVSDRPLSRSEYFEVFLSTLRANGLVAVPTGNGAFRIQPAEGAATNPTRIGSRGA
EEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCC
AQSQMVTEVVRLRTIDAAQAVETLRPLVSKEGAITANKAGNSLVIVDYADNLRRIRALLG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECHHHHHHHHHHHC
DIDRDSTTTQTVILEHAGAREIATALTQLVPAGAEGARALATVVAVDSANAVLMRGDGST
CCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCEEEEECCCHH
LSRLSAMARQLDAKAALGGEIKVVWLDYADSAALVPVLERLIGGQGGGEVASASAAPISL
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEE
GGVGSNATSTAGTQGSTGQAASTGGSSTSPIGATSGGLGNGPIKLANGRGTATITRYPGA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCC
NAIIIAAPADEQRRLSELIRQLDLPQEQVLVEAIIVEISNDVAKELGVQMLFGGKNMPFA
CEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEE
VTNYSNSSPNIIDIAGGLLADNLNQTTTVVNGDTVTTTTNSTAGDLLRENAASKLLSARG
EEECCCCCCCEEEECCCHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHCC
AYTGFLTELSKDTYLGAIINAVQTDKNSNILSTPHITTNNNVPASILFGQEIPVSTGEAL
CHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCEEECCCCCCEEEEECCCCCCCCCCCC
SGGNFSDTFRTIQRQNVGIELDVTPQINASNMIRLDLRQEVSSIAGTVSKTSNELIVNKR
CCCCHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEHH
EIKTTVTVGDREIVALGGLLDDNEQRTLQKVPLLGDIPILGEAFKSRARSRVKTNLMVFI
HEEEEEEECCCEEEEECCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEE
RPTILRNAADRQALAARRYGVVRDAQVKFDSKREPTIDDLIVDYMGATLPVAPSAEVAVV
EHHHHHCCHHHHHHHHHHHCCEECCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCEEEE
APGDTVVRPRAAATPLEPAELPPSSARN
CCCCCEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]