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The map label for this gene is kefA [C]

Identifier: 87200568

GI number: 87200568

Start: 2758043

End: 2759008

Strand: Direct

Name: kefA [C]

Synonym: Saro_2555

Alternate gene names: 87200568

Gene position: 2758043-2759008 (Clockwise)

Preceding gene: 87200567

Following gene: 87200569

Centisome position: 77.44

GC content: 63.56

Gene sequence:

>966_bases
GTGATCCACAACCTGGAGAAAGTGCACCGCGAGGGGCGGAAGCTGGCCCGCGCGCTGGACAAGATCGGGTTCGACATCGG
TCACTACCACGTGTCGGCCTGGAACGCGACGAAAGTGGTGCTGGTCCTTATCGCCGTGATCCTTGTCGCGCGGCTGGGAA
GCAGGCTGACGCGGCGGATCTTCGCGCGCATGTCGAGCCTCGATCGGACGCAGCAGGTGCTGGGCGAGAAGCTTGCCTCG
ATCGCGATCTGGGCACTTGCCCTGTTCGTCGGCATCGACCTGCTGGGGATCAGCCTGACCGCCCTGACGGTGTTTTCCGG
CGCGTTCGGCCTGGCCATCGGCTTTGGCCTGCAGAAGACGTTCGGCAACCTGATCGCCGGGATCATCCTGCTGATGGACC
GTTCGATCAAGCCGGGCGACGTGATCGCGGTGAACGACGGCAAGGGCAGTTCGTTCGGCGAGGTCAAGAAGATCGGCATC
CGGGCGGTATCGGTGACGACGCGCGACAACCGCGAGTATCTGATTCCGAACGAAAACCTGATGACGCAGCAGGTGGAGAA
CTGGTCCTATTCCTCGCGCGAGGTGGCGATCAACATTCCGGTCAGCGTCGCATATGGCTCGGACCTCGACCAGGTCGAGA
AGCTGCTCCTGGAGGCGGTGGGCGCAAGCCCGCGCGCGCTGTCGTTCCCCTCGCCCGGCGTATTGCTGAGCGCGTTCGGC
CCGAGCAGCATCGACATGTCGCTGGTGGTATGGATCGACGATCCGGAAGCGGGCGTGGGCAACGTGCGCTCGGACATCCT
GAAGAAGGTCTGGCGCCTGTTCCGCGACAACGGCGTGCAGATTCCCTTCCCGCAAAGCGACCTGAACCTCAAGGATAGCG
ATGGTCTGCGGGTGTTGGCGGAAGCTTTGCGAGGCCGGATCGACGCTCCTGATGCCTCCAATTCCCGAGAACCACCGTCA
CCCTGA

Upstream 100 bases:

>100_bases
TGGAGGACCGCTTCGGCGTGCGGCTTTCGATCGGGCTGGAGGACACCGCCGACCTCATCGCGGACATCGAGCAGGCGCTT
GGCGCGTGGAGGGCGGCGCT

Downstream 100 bases:

>100_bases
ACTTGTTTCAGGGTCGATGCCTGATGCGGCGCCGTGGTTGGCTGAGAGCGCAACCTGCGGGCATAGTGGCAAGGCGCTAA
CCACCGTATTGTGCCGATGG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MIHNLEKVHREGRKLARALDKIGFDIGHYHVSAWNATKVVLVLIAVILVARLGSRLTRRIFARMSSLDRTQQVLGEKLAS
IAIWALALFVGIDLLGISLTALTVFSGAFGLAIGFGLQKTFGNLIAGIILLMDRSIKPGDVIAVNDGKGSSFGEVKKIGI
RAVSVTTRDNREYLIPNENLMTQQVENWSYSSREVAINIPVSVAYGSDLDQVEKLLLEAVGASPRALSFPSPGVLLSAFG
PSSIDMSLVVWIDDPEAGVGNVRSDILKKVWRLFRDNGVQIPFPQSDLNLKDSDGLRVLAEALRGRIDAPDASNSREPPS
P

Sequences:

>Translated_321_residues
MIHNLEKVHREGRKLARALDKIGFDIGHYHVSAWNATKVVLVLIAVILVARLGSRLTRRIFARMSSLDRTQQVLGEKLAS
IAIWALALFVGIDLLGISLTALTVFSGAFGLAIGFGLQKTFGNLIAGIILLMDRSIKPGDVIAVNDGKGSSFGEVKKIGI
RAVSVTTRDNREYLIPNENLMTQQVENWSYSSREVAINIPVSVAYGSDLDQVEKLLLEAVGASPRALSFPSPGVLLSAFG
PSSIDMSLVVWIDDPEAGVGNVRSDILKKVWRLFRDNGVQIPFPQSDLNLKDSDGLRVLAEALRGRIDAPDASNSREPPS
P
>Mature_321_residues
MIHNLEKVHREGRKLARALDKIGFDIGHYHVSAWNATKVVLVLIAVILVARLGSRLTRRIFARMSSLDRTQQVLGEKLAS
IAIWALALFVGIDLLGISLTALTVFSGAFGLAIGFGLQKTFGNLIAGIILLMDRSIKPGDVIAVNDGKGSSFGEVKKIGI
RAVSVTTRDNREYLIPNENLMTQQVENWSYSSREVAINIPVSVAYGSDLDQVEKLLLEAVGASPRALSFPSPGVLLSAFG
PSSIDMSLVVWIDDPEAGVGNVRSDILKKVWRLFRDNGVQIPFPQSDLNLKDSDGLRVLAEALRGRIDAPDASNSREPPS
P

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=215, Percent_Identity=34.8837209302326, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI2367355, Length=303, Percent_Identity=28.0528052805281, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789291, Length=260, Percent_Identity=28.8461538461538, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787591, Length=267, Percent_Identity=23.9700374531835, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 34848; Mature: 34848

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHNLEKVHREGRKLARALDKIGFDIGHYHVSAWNATKVVLVLIAVILVARLGSRLTRRI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
FARMSSLDRTQQVLGEKLASIAIWALALFVGIDLLGISLTALTVFSGAFGLAIGFGLQKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGNLIAGIILLMDRSIKPGDVIAVNDGKGSSFGEVKKIGIRAVSVTTRDNREYLIPNENL
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCEEEEEEEECCCCEEECCCCCH
MTQQVENWSYSSREVAINIPVSVAYGSDLDQVEKLLLEAVGASPRALSFPSPGVLLSAFG
HHHHHHCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
PSSIDMSLVVWIDDPEAGVGNVRSDILKKVWRLFRDNGVQIPFPQSDLNLKDSDGLRVLA
CCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
EALRGRIDAPDASNSREPPSP
HHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIHNLEKVHREGRKLARALDKIGFDIGHYHVSAWNATKVVLVLIAVILVARLGSRLTRRI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
FARMSSLDRTQQVLGEKLASIAIWALALFVGIDLLGISLTALTVFSGAFGLAIGFGLQKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGNLIAGIILLMDRSIKPGDVIAVNDGKGSSFGEVKKIGIRAVSVTTRDNREYLIPNENL
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCEEEEEEEECCCCEEECCCCCH
MTQQVENWSYSSREVAINIPVSVAYGSDLDQVEKLLLEAVGASPRALSFPSPGVLLSAFG
HHHHHHCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECC
PSSIDMSLVVWIDDPEAGVGNVRSDILKKVWRLFRDNGVQIPFPQSDLNLKDSDGLRVLA
CCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
EALRGRIDAPDASNSREPPSP
HHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]