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The map label for this gene is rpoA

Identifier: 87200540

GI number: 87200540

Start: 2730746

End: 2731807

Strand: Reverse

Name: rpoA

Synonym: Saro_2527

Alternate gene names: 87200540

Gene position: 2731807-2730746 (Counterclockwise)

Preceding gene: 87200541

Following gene: 87200539

Centisome position: 76.7

GC content: 61.21

Gene sequence:

>1062_bases
ATGACTGTCAACATCAAGAACTGGCAGGAACTGAAGAAGCCCAACAATCTCGAGATCAAGCCGGGCAACGATTCCAAGCG
CCGTGCGACTTTCGTCGCCGAGCCGCTTGAGCGTGGTTTTGGTCTCACGCTCGGCAACGCGCTGCGTCGCGTGCTGCTCT
CCTCGCTTCAGGGCGCGGCGGTCACCTCGATCAAGATCGAGAACGTTCTGCACGAATTCTCGTCGCTCGCCGGCGTGCGC
GAGGACGTCACCGACATCGTGCTCAACGTCAAGCAGATCGCGCTCAAGATGCAGGGCGAAGGCCCCAAGCGTCTCCAGCT
TTCGGCAACCGGTCCTGGCGAAGTCAAGGCCGGCGACATTGCCGTGACGGGCGACATCGAGGTCATGAACAAGGACCTCG
TGATCTGCCATCTCGACGAAGGCGCGACCTTCAACATGGAACTGACCGTCGATACCGGCAAGGGCTACGTCCCCGCCGTG
TCGAACCGTCCGGTAGACGCTCCGATCGGTCTGATCCCGGTTGACTCGCTCTATTCGCCGGTCCGTCAGGTCTCCTACAA
GGTCGAGAACGCCCGCATCGGTCAGGAGCTTGACTACGACAAGCTCAACCTGACGGTCGAAACCGACGGCACCGTCACTC
CGGAAGACGCCGTGGCCTATGCCGCGCGCATCCTTCAGGACCAGCTTGCGCTGTTCGTCCACTTCGACGACCAGGTTCCG
GTCGGCCACGTTCCGATGGTCGCGGGCGTTCCTGCCCACGCGCCGGAGGAAAGCGACGCGAACCAGCTCAACCGCTACCT
TCTCAAGAAGGTGGACGAGCTGGAACTGTCGGTTCGCTCGGCCAACTGCCTCAAGAACGACAACATCATCTACATCGGCG
ACCTGGTCCAGAAGACCGAAGCCGAGATGCTGCGCACGCCGAACTTCGGCCGCAAGTCGCTCAACGAGATCAAGGAAGTT
CTCTCCTCGATGGGTCTGCGCCTCGGCATGGACATCCCCGGCTGGCCGCCGGAGAACATCGAAGAGATGGCCAAGAAGCT
CGAACAAGAGCTTCTGGGCTAA

Upstream 100 bases:

>100_bases
ACGGGGCGCAGGTACCGGCTCCCCGTTCGCCCAATACCCGACCGGATGCGGACCTGACGTCCGACATCCGGCAAGCCCGC
ACAAACCCCAGGGGAATTCC

Downstream 100 bases:

>100_bases
GGAAATTCGGGGCGTCCTTTACGGGGCGCCCTGTTTTCAAGGGTGACTTGGCGGCAGCCCGCAATCGCCGCCTGGATCGG
GGTACCTCGCACGGCCCCCC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTVNIKNWQELKKPNNLEIKPGNDSKRRATFVAEPLERGFGLTLGNALRRVLLSSLQGAAVTSIKIENVLHEFSSLAGVR
EDVTDIVLNVKQIALKMQGEGPKRLQLSATGPGEVKAGDIAVTGDIEVMNKDLVICHLDEGATFNMELTVDTGKGYVPAV
SNRPVDAPIGLIPVDSLYSPVRQVSYKVENARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLALFVHFDDQVP
VGHVPMVAGVPAHAPEESDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEV
LSSMGLRLGMDIPGWPPENIEEMAKKLEQELLG

Sequences:

>Translated_353_residues
MTVNIKNWQELKKPNNLEIKPGNDSKRRATFVAEPLERGFGLTLGNALRRVLLSSLQGAAVTSIKIENVLHEFSSLAGVR
EDVTDIVLNVKQIALKMQGEGPKRLQLSATGPGEVKAGDIAVTGDIEVMNKDLVICHLDEGATFNMELTVDTGKGYVPAV
SNRPVDAPIGLIPVDSLYSPVRQVSYKVENARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLALFVHFDDQVP
VGHVPMVAGVPAHAPEESDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEV
LSSMGLRLGMDIPGWPPENIEEMAKKLEQELLG
>Mature_352_residues
TVNIKNWQELKKPNNLEIKPGNDSKRRATFVAEPLERGFGLTLGNALRRVLLSSLQGAAVTSIKIENVLHEFSSLAGVRE
DVTDIVLNVKQIALKMQGEGPKRLQLSATGPGEVKAGDIAVTGDIEVMNKDLVICHLDEGATFNMELTVDTGKGYVPAVS
NRPVDAPIGLIPVDSLYSPVRQVSYKVENARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLALFVHFDDQVPV
GHVPMVAGVPAHAPEESDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVL
SSMGLRLGMDIPGWPPENIEEMAKKLEQELLG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=336, Percent_Identity=47.3214285714286, Blast_Score=295, Evalue=4e-81,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_NOVAD (Q2G5B0)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497797.1
- ProteinModelPortal:   Q2G5B0
- STRING:   Q2G5B0
- GeneID:   3916848
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2527
- NMPDR:   fig|48935.1.peg.3133
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- PhylomeDB:   Q2G5B0
- ProtClustDB:   PRK05182
- BioCyc:   NARO279238:SARO_2527-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38728; Mature: 38597

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNIKNWQELKKPNNLEIKPGNDSKRRATFVAEPLERGFGLTLGNALRRVLLSSLQGAA
CEECCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCE
VTSIKIENVLHEFSSLAGVREDVTDIVLNVKQIALKMQGEGPKRLQLSATGPGEVKAGDI
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCEEECCE
AVTGDIEVMNKDLVICHLDEGATFNMELTVDTGKGYVPAVSNRPVDAPIGLIPVDSLYSP
EEECCCEEECCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHH
VRQVSYKVENARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLALFVHFDDQVP
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCC
VGHVPMVAGVPAHAPEESDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHHCCCCCCCEEEEHHHHHHHH
AEMLRTPNFGRKSLNEIKEVLSSMGLRLGMDIPGWPPENIEEMAKKLEQELLG
HHHHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVNIKNWQELKKPNNLEIKPGNDSKRRATFVAEPLERGFGLTLGNALRRVLLSSLQGAA
EECCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCE
VTSIKIENVLHEFSSLAGVREDVTDIVLNVKQIALKMQGEGPKRLQLSATGPGEVKAGDI
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCEEECCE
AVTGDIEVMNKDLVICHLDEGATFNMELTVDTGKGYVPAVSNRPVDAPIGLIPVDSLYSP
EEECCCEEECCCEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHH
VRQVSYKVENARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLALFVHFDDQVP
HHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCC
VGHVPMVAGVPAHAPEESDANQLNRYLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEHHHCCCCCCCEEEEHHHHHHHH
AEMLRTPNFGRKSLNEIKEVLSSMGLRLGMDIPGWPPENIEEMAKKLEQELLG
HHHHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA