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The map label for this gene is ligE [H]

Identifier: 87200418

GI number: 87200418

Start: 2573603

End: 2574442

Strand: Direct

Name: ligE [H]

Synonym: Saro_2405

Alternate gene names: 87200418

Gene position: 2573603-2574442 (Clockwise)

Preceding gene: 87200417

Following gene: 87200423

Centisome position: 72.26

GC content: 64.52

Gene sequence:

>840_bases
ATGGCCGCCAACAACACGATCACTTTCTACGACCTTGCCCTTTCGACCGGCGCGACGATCAGCCCGTTCGTCTGGGCGAC
GAAATATGCGTTGAAGCACAAGGGCTTCGACCTCGACGTCGTGCCAGGCGGGTTCACCGGAATTCTCGAACGCACCGGCG
GCAAGACCGAGCGCCTTCCCGCGATCGTCGACGACGGCGAGTTCGTTCTCGATAGCTGGGGCATCGTCGAATACCTCGAT
GCCAAGTATCCGGACCGCCCTGTCCTGATCCCGCACGAAAGCGTCGCGGCGACGCTCAAGGCGCTCGACAACTGGTTCTG
GAATGCTGCCGTCGGACCGTGGATGTTCTGCTTCTGCCAGGACTACCGCGATCTCTCGCTGCCGCAGGATCACGAATACG
TCACCCACAGCCGCGAGAAGATGCTCGGCCGCAAGCTCGAAGAAGTCCAGGCCGGACGCGAAGAGCGCCTGCCGAAGATC
TCCGCCGCGCTCGAACCGCTGCGCGCCGCGCTTGCCCAGCACCAGTGGCTCGGCGGCTCCTCGCCCAACTACGCCGACTA
CCGCATCATGGGCGGCATCCTGTTCACCGCCTCGGTGTGCAAGACGCCGGTGCTCGCCAATGACGATCCATTGCGCGACT
GGATCGAGCGCTGCCTCGACCTCTACGGCGGCCTGGGCCGTCACCCCGGGCTGTTCCCGCTGTTCGGCCTGGAACAGCGC
GAAGGCGATCCCGACCTGTTCAATCGCGCGGCAGGCCAGGGCGGCATCTACAAGCGCAACACCGGCCCGGAATCCACCCG
CGCCGAAACCCAGCGCATCACCGAAGGCATGAAGAAGTAA

Upstream 100 bases:

>100_bases
CCCTGAAACAAGTTCAGGGTGACGGCAATTGAGTCACAAATTTCGTTTGTGTTACTAACAATATCTGGCAGACTGCTCGC
AACGACGAGAGAGGATTCCC

Downstream 100 bases:

>100_bases
ACGGGTGGGGCCGGAGCGATCCGGCCCCTATTTCCTGAGCCCTTGTTTCCCGAGCTTGGCCATCATGTCGCCGAAGGTCA
GGCGAGTGTCGATCGTGGTA

Product: glutathione S-transferase-like protein

Products: NA

Alternate protein names: Beta-aryl ether cleaving enzyme [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MAANNTITFYDLALSTGATISPFVWATKYALKHKGFDLDVVPGGFTGILERTGGKTERLPAIVDDGEFVLDSWGIVEYLD
AKYPDRPVLIPHESVAATLKALDNWFWNAAVGPWMFCFCQDYRDLSLPQDHEYVTHSREKMLGRKLEEVQAGREERLPKI
SAALEPLRAALAQHQWLGGSSPNYADYRIMGGILFTASVCKTPVLANDDPLRDWIERCLDLYGGLGRHPGLFPLFGLEQR
EGDPDLFNRAAGQGGIYKRNTGPESTRAETQRITEGMKK

Sequences:

>Translated_279_residues
MAANNTITFYDLALSTGATISPFVWATKYALKHKGFDLDVVPGGFTGILERTGGKTERLPAIVDDGEFVLDSWGIVEYLD
AKYPDRPVLIPHESVAATLKALDNWFWNAAVGPWMFCFCQDYRDLSLPQDHEYVTHSREKMLGRKLEEVQAGREERLPKI
SAALEPLRAALAQHQWLGGSSPNYADYRIMGGILFTASVCKTPVLANDDPLRDWIERCLDLYGGLGRHPGLFPLFGLEQR
EGDPDLFNRAAGQGGIYKRNTGPESTRAETQRITEGMKK
>Mature_278_residues
AANNTITFYDLALSTGATISPFVWATKYALKHKGFDLDVVPGGFTGILERTGGKTERLPAIVDDGEFVLDSWGIVEYLDA
KYPDRPVLIPHESVAATLKALDNWFWNAAVGPWMFCFCQDYRDLSLPQDHEYVTHSREKMLGRKLEEVQAGREERLPKIS
AALEPLRAALAQHQWLGGSSPNYADYRIMGGILFTASVCKTPVLANDDPLRDWIERCLDLYGGLGRHPGLFPLFGLEQRE
GDPDLFNRAAGQGGIYKRNTGPESTRAETQRITEGMKK

Specific function: Able to degrade various dimeric lignin compounds. Catalyzes the unique and reductive cleavage of arylglycerol-beta- aryl ether [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 31123; Mature: 30991

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANNTITFYDLALSTGATISPFVWATKYALKHKGFDLDVVPGGFTGILERTGGKTERLP
CCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCCC
AIVDDGEFVLDSWGIVEYLDAKYPDRPVLIPHESVAATLKALDNWFWNAAVGPWMFCFCQ
EEECCCCEEEECCCHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYRDLSLPQDHEYVTHSREKMLGRKLEEVQAGREERLPKISAALEPLRAALAQHQWLGGS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCC
SPNYADYRIMGGILFTASVCKTPVLANDDPLRDWIERCLDLYGGLGRHPGLFPLFGLEQR
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EGDPDLFNRAAGQGGIYKRNTGPESTRAETQRITEGMKK
CCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AANNTITFYDLALSTGATISPFVWATKYALKHKGFDLDVVPGGFTGILERTGGKTERLP
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHCCCCCCCCC
AIVDDGEFVLDSWGIVEYLDAKYPDRPVLIPHESVAATLKALDNWFWNAAVGPWMFCFCQ
EEECCCCEEEECCCHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DYRDLSLPQDHEYVTHSREKMLGRKLEEVQAGREERLPKISAALEPLRAALAQHQWLGGS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCC
SPNYADYRIMGGILFTASVCKTPVLANDDPLRDWIERCLDLYGGLGRHPGLFPLFGLEQR
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EGDPDLFNRAAGQGGIYKRNTGPESTRAETQRITEGMKK
CCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726 [H]